七姊妹山自然保护区蕨类植物区系地理及资源开发研究

运用植物区系地理分析方法对七姊妹山自然保护区的蕨类植物区系特征进行研究。科的地理区系分析表明,热带、亚热带成分高达58.3%,占主导地位。属的地理区系分析也表明了类似的趋势。种的地理区系分析表明,除了热带、亚热带成分占有较高比例外,温带分布的种也具有较高的比例,占非世界广布种的1.2%。特别是世界温带分布的种占较高比例,达24.2%,这反映了温带成分的重要地位。七姊妹山蕨类植物资源丰富,可区分为药用、观赏、食用、指示类植物等几大类。各种蕨类植物资源显示了广阔的利用前景。     

A newly isolated fungal strain used as whole‐cell biocatalyst for biodiesel production from palm oil

A strain of Aspergillus niger isolated from atmospherically exposed bread and Jatropha curcas seed was utilized as a whole‐cell biocatalyst for palm oil methanolysis to produce fatty acid methyl esters (FAME), or biodiesel. The A. niger strain had a lipase activity of 212.58 mU mL^?1 after 144 h incubation at 25 °C with an initial pH value of 6.5, using 7% polypeptone (w/w on basal medium) as the nitrogen source and 3% olive oil (w/w on basal medium) as a carbon source. The A. niger cells spontaneously immobilized within polyurethane biomass support particles (BSPs) during submerged fermentation. Thereafter, the methanolysis of palm oil was achieved via a three‐step addition of methanol in the presence of BSPs‐immobilized with A. niger cells. The influence of water content, reaction temperature and enzyme concentration on reaction rate was investigated. An 8% water content and a temperature of 40 °C in the presence of 30 immobilized BSPs, resulted in an 87% FAME yield after 72 h.     

Diel pattern in the structure and function of the gut microbial community in Lymantria dispar asiatica (Lepidoptera: Lymantriidae) larvae

In the present study, diel pattern in gut microbial communities in insects were evaluated. Lymantria dispar asiatica fourth instar larvae (72 ± 2 hr after molting) at noon (LdD) and midnight (LdN) were used for a comparative analysis of the gut microbial community. Ten bacterial operational taxonomic units (OTUs) were shared between LdD and LdN samples. One bacterial OTU was specific to LdD. The dominant gut microbes were OTU72 in LdD and OTU75 in LdN. A linear discriminant analysis effect size cladogram suggested that ten bacterial OTUs maintain significant differences in relative abundances between LdD and LdN. These results agreed with the discrete ellipses between LdD and LdN in principal coordinates analysis plots. Additionally, using phylogenetic investigation of communities by reconstruction of unobserved states, the gut microbial community was assigned to 23 functional terms, among which 22 exhibited significant differences between LdD and LdN. To conclude, the present study documented a diel pattern in the gut microbial community of L. dispar asiatica larvae.     

不同层流净化级别室内微量麻醉气体对医护人员的影响

目的:研究净化级别不同室内麻醉药物残留浓度对医护人员健康的影响,为制定麻醉操作规范提供参考依据。方法:通过Tedlar采样袋采集麻醉医生及麻醉恢复室人员呼出气20 mL,双盲法送30 min内相关人员应用气相色谱法进行分析。结果:不同层流净化级别室内医务人员呼出微量麻醉气体的浓度不同。十万级恢复室内医护人员呼出气体的浓度高于百级和万级手术间。结论:麻醉废气污染对手术室工作人员的心理行为及操作能力产生影响,医护人员应增强自我防护意识,定期监测手术室环境污染的程度,制定预防和减少麻醉废气污染的有效措施。     

Elevated expression of CUEDC2 protein confers endocrine resistance in breast cancer

Endocrine resistance is a major obstacle to hormonal therapy for breast cancers. Although reduced expression of estrogen receptor-α (ER-α) is a known contributing factor to endocrine resistance, the mechanism of ER-α downregulation in endocrine resistance is still not fully understood. Here we report that CUE domain-containing protein-2 (CUEDC2), a ubiquitin-binding motif-containing protein, is a key factor in endocrine resistance in breast cancer. We show that CUEDC2 modulates ER-α protein stability through the ubiquitin-proteasome pathway. Through the study of specimens from a large cohort of subjects with breast cancer, we found a strong inverse correlation between CUEDC2 and ER-α protein expression. Notably, subjects with tumors that highly expressed CUEDC2 had poor responsiveness to tamoxifen treatment and high potential for relapse. We further show that ectopic CUEDC2 expression impaired the responsiveness of breast cancer cells to tamoxifen. Therefore, our findings suggest that CUEDC2 is a crucial determinant of resistance to endocrine therapies in breast cancer.     

祁连圆柏光合作用日变化特征及其与生理生态因子的关系

以吐鲁沟国家森林公园内百年左右树龄的祁连圆柏为对象,采用Li-6400光合作用测定系统,在自然环境条件下测定了其生长季内各月份生理生态因子和净光合速率的日变化,探讨祁连圆柏生理生态特征以及与其生理生态因子间的关系.结果表明,在整个生长季内,祁连圆柏的净光合速率日变化为双峰型曲线,其在10:00和16:00达到峰值,于14:00达到最小值,光合作用存在明显的午休现象",而月份之间的净光合速率表现为8月>5月>6月>7月.祁连圆柏的气孔限制值与净光合速率有相似的日进程,呈现出M"型的双峰曲线,分别在10:00和16:00达到峰值,而胞间CO2浓度日变化趋势与气孔限制值相反,表现出W"型双峰曲线.水分利用效率与净光合速率有相同的日变化趋势,其在整个生长季内表现为5月>8月>7月>6月.在整个生长季内,影响祁连圆柏光合变化的主要决定生理生态因子是胞间CO2浓度和气温,限制生理生态因子是气孔导度和光合有效辐射强度.     

de novo Design and Synthesis of Candida antarctica Lipase B Gene and α-Factor Leads to High-Level Expression in Pichia pastoris

Candida antarctica lipase B (CALB) is one of the most widely used and studied enzymes in the world. In order to achieve the high-level expression of CALB in Pichia, we optimized the codons of CALB gene and α-factor by using a de novo design and synthesis strategy. Through comparative analysis of a series of recombinants with different expression components, we found that the methanol-inducible expression recombinant carrying the codon-optimized α-factor and mature CALB gene (pPIC9KαM-CalBM) has the highest lipase production capacity. After fermentation parameters optimization, the lipase activity and protein content of the recombinant pPIC9KαM-CalBM reached 6,100 U/mL and 3.0 g/L, respectively, in a 5-L fermentor. We believe this strategy could be of special interest due to its capacity to improve the expression level of target gene, and the Pichia transformants carrying the codon-optimized gene had great potential for the industrial-scale production of CALB lipase.     

电和L—谷氨酸钠刺激兔中缝隐核对膈神经放电的影响

实验在45只麻醉、自主呼吸、断双侧颈迷走神经的家兔上进行。电刺激或微量注射L-谷氨酸钠于中缝隐核(Nucleus raphe obscurus,NRO),观察到:(1)长串电脉冲刺激NRO(50—200μA,波宽0.3ms,100Hz,4—6s),出现膈神经放电被抑制的反应,被抑制的程度与刺激强度、刺激频率间存在相关性。(2)吸气期用短串电脉冲(100—200μA,波宽0.3ms,50—100Hz,5—20个脉冲)刺激NRO,可提前终止膈神经放电,产生吸气切断效应。吸气切断时间具有刺激落位和刺激强度依赖性。(3)NRO内微量注射细胞体兴奋剂谷氨酸钠(1mol/L,1μl),注药期间出现膈神经放电抑制,注药后为吸气时程(Ti)缩短和呼气时程(Te)延长。