全文获取类型
收费全文 | 1794篇 |
免费 | 140篇 |
出版年
2023年 | 5篇 |
2022年 | 5篇 |
2021年 | 18篇 |
2020年 | 13篇 |
2019年 | 13篇 |
2018年 | 18篇 |
2017年 | 20篇 |
2016年 | 41篇 |
2015年 | 63篇 |
2014年 | 94篇 |
2013年 | 106篇 |
2012年 | 154篇 |
2011年 | 197篇 |
2010年 | 131篇 |
2009年 | 99篇 |
2008年 | 107篇 |
2007年 | 87篇 |
2006年 | 80篇 |
2005年 | 67篇 |
2004年 | 89篇 |
2003年 | 83篇 |
2002年 | 77篇 |
2001年 | 17篇 |
2000年 | 16篇 |
1999年 | 19篇 |
1998年 | 34篇 |
1997年 | 10篇 |
1996年 | 18篇 |
1995年 | 18篇 |
1994年 | 17篇 |
1993年 | 12篇 |
1992年 | 12篇 |
1991年 | 18篇 |
1990年 | 15篇 |
1989年 | 9篇 |
1988年 | 14篇 |
1987年 | 9篇 |
1985年 | 13篇 |
1984年 | 15篇 |
1983年 | 9篇 |
1982年 | 10篇 |
1981年 | 8篇 |
1980年 | 6篇 |
1979年 | 5篇 |
1978年 | 8篇 |
1977年 | 5篇 |
1976年 | 7篇 |
1974年 | 6篇 |
1972年 | 7篇 |
1971年 | 4篇 |
排序方式: 共有1934条查询结果,搜索用时 281 毫秒
61.
Deborah M. Ramsey Md. Amirul Islam Lynne Turnbull Rohan A. Davis Cynthia B. Whitchurch Shelli R. McAlpine 《Bioorganic & medicinal chemistry letters》2013,23(17):4862-4866
Described is the antibiotic activity of a marine natural product. Psammaplysin F (1) inhibited the growth of four Gram-positive strains by >80% at 50 μM, and the amine at position C-20 is responsible for the observed antibacterial activity. When tested against two strains of methicillin resistant Staphylococcus aureus (MRSA), the minimum inhibitory concentrations (MICs) for psammaplysin F (40–80 μM) were similar to the structurally-related alkaloid psammaplysin H (2). Psammaplysin F (1) increased membrane permeability by two to four-fold compared to psammaplysin H (2) or control-treated bacteria, respectively. Unlike psammaplysin H (2), we show that psammaplysin F (1) inhibits equal partitioning of DNA into each daughter cell, suggesting that this natural product is a unique prokaryotic cell division inhibitor. 相似文献
62.
63.
Martin H. van der Meer John B. Horne Michael G. Gardner Jean‐Paul A. Hobbs Morgan Pratchett Lynne van Herwerden 《Ecology and evolution》2013,3(6):1653-1666
Extensive ongoing degradation of coral reef habitats worldwide has lead to declines in abundance of coral reef fishes and local extinction of some species. Those most vulnerable are ecological specialists and endemic species. Determining connectivity between locations is vital to understanding recovery and long‐term persistence of these species following local extinction. This study explored population connectivity in the ecologically‐specialized endemic three‐striped butterflyfish (Chaetodon tricinctus) using mt and msatDNA (nuclear microsatellites) to distinguish evolutionary versus contemporary gene flow, estimate self‐replenishment and measure genetic diversity among locations at the remote Australian offshore coral reefs of Middleton Reef (MR), Elizabeth Reef (ER), Lord Howe Island (LHI), and Norfolk Island (NI). Mt and msatDNA suggested genetic differentiation of the most peripheral location (NI) from the remaining three locations (MR, ER, LHI). Despite high levels of mtDNA gene flow, there is limited msatDNA gene flow with evidence of high levels of self‐replenishment (≥76%) at all four locations. Taken together, this suggests prolonged population recovery times following population declines. The peripheral population (NI) is most vulnerable to local extinction due to its relative isolation, extreme levels of self‐replenishment (95%), and low contemporary abundance. 相似文献
64.
Jing Lu Dee A. Carter Lynne Turnbull Douglas Rosendale Duncan Hedderley Jonathan Stephens Swapna Gannabathula Gregor Steinhorn Ralf C. Schlothauer Cynthia B. Whitchurch Elizabeth J. Harry 《PloS one》2013,8(2)
Treatment of chronic wounds is becoming increasingly difficult due to antibiotic resistance. Complex natural products with antimicrobial activity, such as honey, are now under the spotlight as alternative treatments to antibiotics. Several studies have shown honey to have broad-spectrum antibacterial activity at concentrations present in honey dressings, and resistance to honey has not been attainable in the laboratory. However not all honeys are the same and few studies have used honey that is well defined both in geographic and chemical terms. Here we have used a range of concentrations of clover honey and a suite of manuka and kanuka honeys from known geographical locations, and for which the floral source and concentration of methylglyoxal and hydrogen peroxide potential were defined, to determine their effect on growth and cellular morphology of four bacteria: Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. While the general trend in effectiveness of growth inhibition was manuka>manuka-kanuka blend>kanuka>clover, the honeys had varying and diverse effects on the growth and cellular morphology of each bacterium, and each organism had a unique response profile to these honeys. P. aeruginosa showed a markedly different pattern of growth inhibition to the other three organisms when treated with sub-inhibitory concentrations of honey, being equally sensitive to all honeys, including clover, and the least sensitive to honey overall. While hydrogen peroxide potential contributed to the antibacterial activity of the manuka and kanuka honeys, it was never essential for complete growth inhibition. Cell morphology analysis also showed a varied and diverse set of responses to the honeys that included cell length changes, cell lysis, and alterations to DNA appearance. These changes are likely to reflect the different regulatory circuits of the organisms that are activated by the stress of honey treatment. 相似文献
65.
66.
David P. Dimmock Michelle M. Clark Mary Gaughran Julie A. Cakici Sara A. Caylor Christina Clarke Michele Feddock Shimul Chowdhury Lisa Salz Cynthia Cheung Lynne M. Bird Charlotte Hobbs Kristen Wigby Lauge Farnaes Cinnamon S. Bloss Stephen F. Kingsmore the RCIGM Investigators 《American journal of human genetics》2020,107(5):942
67.
68.
Randomized controlled trial of a nationally available weight control program tailored for adults with type 2 diabetes
下载免费PDF全文
![点击此处可从《Obesity (Silver Spring, Md.)》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Patrick M. O'Neil Peter W. Tuerk Lynne E. Becker Thomas A. Wadden Ken Fujioka Priscilla L. Hollander Robert F. Kushner W. Timothy Garvey Domenica M. Rubino Robert J. Malcolm Daniel Weiss William J. Raum Jonny L. Salyer Kathie L. Hermayer Jan L. Veliko Nicoleta D. Sora 《Obesity (Silver Spring, Md.)》2016,24(11):2269-2277
69.
A Window into brain development: hdEEG methods to track visual development in nonhuman primates
下载免费PDF全文
![点击此处可从《Developmental neurobiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Electroencephalography (EEG) is widely used to study human brain activity, and is a useful tool for bridging the gap between invasive neural recording assays and behavioral data. High‐density EEG (hdEEG) methods currently used for human subjects for use with infant macaque monkeys, a species that exhibits similar visual development to humans over a shorter time course was adapted. Unlike monkeys, human subjects were difficult to study longitudinally and were not appropriate for direct within‐species comparison to neuronal data. About 27‐channel electrode caps, which allowed collection of hdEEG data from infant monkeys across development were designed. Acuity and contrast sweep VEP responses to grating stimuli was obtained and a new method for objective threshold estimation based on response signal‐to‐noise ratios at different stimulus levels was established. The developmental trajectories of VEP‐measured contrast sensitivity and acuity to previously collected behavioral and neuronal data were compared. The VEP measures showed similar rates of development to behavioral measures, both of which were slower than direct neuronal measures; VEP thresholds were higher than other measures. This is the first usage of non‐invasive technology in non‐human primates. Other means to assess neural sensitivity in infants were all invasive. Use of hdEEG with infant monkeys opens many possibilities for tracking development of vision and other functions in non‐human primates, and can expand our understanding of the relationship between neuronal activity and behavioral capabilities across various sensory and cognitive domains. © 2016 Wiley Periodicals, Inc. Develop Neurobiol 76: 1342–1359, 2016 相似文献
70.
Emily Sellens Jacqueline M. Norris Navneet K. Dhand Jane Heller Lynne Hayes Heather F. Gidding Harold Willaby Nicholas Wood Katrina L. Bosward 《PloS one》2016,11(1)
Q fever, caused by Coxiella burnetii, is a serious zoonotic disease in humans with a worldwide distribution. Many species of animals are capable of transmitting C. burnetii, and consequently all veterinary workers are at risk for this disease. An effective Q fever vaccine has been readily available and used in Australia for many years in at-risk groups, and the European Centre for Disease Prevention and Control has recently also called for the use of this vaccine among at-risk groups in Europe. Little is known about attitudes towards this vaccine and vaccine uptake in veterinary workers. This study aimed to determine the Q fever vaccination status of veterinarians and veterinary nurses in Australia and to assess and compare the knowledge and attitudes towards Q fever disease and vaccination of each cohort. An online cross-sectional survey performed in 2014 targeted all veterinarians and veterinary nurses in Australia. Responses from 890 veterinarians and 852 veterinary nurses were obtained. Binary, ordinal and multinomial logistic regression were used to make comparisons between the two cohorts. The results showed that 74% of veterinarians had sought vaccination compared to only 29% of veterinary nurses. Barriers to vaccination among those not vaccinated did not differ between cohorts, and included a lack of perceived risk, financial expense, time constraints, and difficulty in finding a vaccine provider. Poor knowledge and awareness of Q fever disease and vaccination were additional and notable barriers for the veterinary nursing cohort, suggesting veterinary clinics and veterinarians may not be meeting their legal responsibility to educate staff about risks and risk prevention. Further evaluation is needed to identify the drivers behind seeking and recommending vaccination so that recommendations can be made to improve vaccine uptake. 相似文献