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51.
Bussenius J Anand NK Blazey CM Bowles OJ Bannen LC Chan DS Chen B Co EW Costanzo S DeFina SC Dubenko L Engst S Franzini M Huang P Jammalamadaka V Khoury RG Kim MH Klein RR Laird D Le DT Mac MB Matthews DJ Markby D Miller N Nuss JM Parks JJ Tsang TH Tsuhako AL Wang Y Xu W Rice KD 《Bioorganic & medicinal chemistry letters》2012,22(6):2283-2286
52.
The porous properties of brain tissue are important for understanding normal and abnormal cerebrospinal fluid flow in the brain. In this study, a poroviscoelastic model was fitted to the stress relaxation response of white matter in unconfined compression performed under a range of low strain rates. A set of experiments was also performed on the tissue samples using a no-slip boundary condition. Results from these experiments demonstrated that the rheological response of the white matter is primarily governed by the intrinsic viscoelastic properties of the solid phase. The permeability of white matter was found to be of the order of 10(-12) m4/Ns. 相似文献
53.
Selwood L 《IUBMB life》2007,59(10):617-621
An analysis of several features of marsupial oocytes and developmental stages from the zygote to the neonate shows the advantages of studying these to answer questions about mammalian development in general. Marsupials make good model systems providing that species have well established breeding and husbandry protocols, a detailed time table of embryonic development associated with appropriate monitoring to collect desired stages and an induced ovulation protocol to allow collection of normal oocytes and developmental stages. One of the specific features of marsupial development that would provide further insight into problems in mammalian development is distinct oocyte and/or zygote polarity. Additionally, because the conceptus is transparent, cell-cell associations can be studied until well after the hypoblast is formed. These combined features mean that lineage allocation and axis formation can be more easily studied and related to oocyte/zygote polarity and the order of cell division in these conceptuses. Another feature is the ability to collect normal unattached embryonic and fetal stages in some species and to culture conceptuses from the primitive streak stage to within a few hours of birth. This ability means that stages of organogenesis in most organ systems are readily accessible without the complications of implantation. 相似文献
54.
Palmer CA Watts RA Houck LD Picard AL Arnold SJ 《Evolution; international journal of organic evolution》2007,61(1):202-215
In this article we explore the evolutionary history of a functional complex at the molecular level in plethodontid salamanders. The complex consists of a proteinaceous courtship pheromone, a pheromone-producing gland on the male's chin, and a set of behaviors for delivering the pheromone to the female. Long-term evolutionary stasis is the defining feature of this complex at both the morphological and behavioral levels. However, our previous assessment of the pheromone gene, plethodontid receptivity factor (PRF), revealed rapid evolution at the molecular level despite stasis at higher levels of organization. Analysis of a second pheromone gene, sodefrin precursor-like factor (SPF), now indicates that evolutionary decoupling in this complex is pervasive. The evolutionary profiles of SPF and PRF are remarkably similar in that: (a) both genes exhibit high levels of sequence diversity both within and across taxa, (b) genetic diversity has been driven by strong positive selection, and (c) the genes have evolved heterogeneously in different salamander lineages. The composition of the pheromone signal as a whole, however, has experienced an extraordinary evolutionary transition. Whereas SPF has been retained throughout the 100 MY radiation of salamanders, PRF has only recently been recruited to a pheromone function (27 million years ago). When SPF and PRF coexist in the same clade, they show contrasting patterns of evolution. When one shows rapid evolution driven by positive selection, the other shows neutral divergence restrained by purifying selection. In one clade, the origin and subsequent rapid evolution of PRF appear to have interfered with the evolution and persistence of SPF, leading to a pattern of evolutionary replacement. Overall, these two pheromone genes provide a revealing window on the dynamics that drive the evolution of multiple traits in a signaling complex. 相似文献
55.
Chou YH Flitney FW Chang L Mendez M Grin B Goldman RD 《Experimental cell research》2007,313(10):2236-2243
Intermediate filament (IF) proteins exist in multiple structural forms within cells including mature IF, short filaments or 'squiggles', and non-filamentous precursors called particles. These forms are interconvertible and their relative abundance is IF type, cell type- and cell cycle stage-dependent. These structures are often associated with molecular motors, such as kinesin and dynein, and are therefore capable of translocating through the cytoplasm along microtubules. The assembly of mature IF from their precursor particles is also coupled to translation. These dynamic properties of IF provide mechanisms for regulating their reorganization and assembly in response to the functional requirements of cells. The recent findings that IF and their precursors are frequently associated with signaling molecules have revealed new functions for IF beyond their more traditional roles as mechanical integrators of cells and tissues. 相似文献
56.
Anderson I Risso C Holmes D Lucas S Copeland A Lapidus A Cheng JF Bruce D Goodwin L Pitluck S Saunders E Brettin T Detter JC Han C Tapia R Larimer F Land M Hauser L Woyke T Lovley D Kyrpides N Ivanova N 《Standards in genomic sciences》2011,5(1):50-60
Ferroglobus placidus belongs to the order Archaeoglobales within the archaeal phylum Euryarchaeota. Strain AEDII12DO is the type strain of the species and was isolated from a shallow marine hydrothermal system at Vulcano, Italy. It is a hyperthermophilic, anaerobic chemolithoautotroph, but it can also use a variety of aromatic compounds as electron donors. Here we describe the features of this organism together with the complete genome sequence and annotation. The 2,196,266 bp genome with its 2,567 protein-coding and 55 RNA genes was sequenced as part of a DOE Joint Genome Institute Laboratory Sequencing Program (LSP) project. 相似文献
57.
Riglar DT Richard D Wilson DW Boyle MJ Dekiwadia C Turnbull L Angrisano F Marapana DS Rogers KL Whitchurch CB Beeson JG Cowman AF Ralph SA Baum J 《Cell host & microbe》2011,9(1):9-20
Highlights? Merozoite attachment signals commitment to invasion and triggers subsequent events ? PfAMA1 and PfRON4 define core components of the merozoite-erythrocyte tight junction ? A ring of actin forms at the tight junction midway through merozoite invasion 相似文献
58.
Lynne A. Isbell Jill D. Pruetz Michelle Lewis Truman P. Young 《International journal of primatology》1999,20(2):257-272
One of the central dichotomies in primate behavior is between species in which there are relationships among females that include stable dominance relationships, and those in which the relationships include weak or unstable dominance relationships. This dichotomy has been attributed to differences in food resources, with stable dominance hierarchies occurring in species that feed on usurpable foods. We compared rank-related differences in nonagonistic behaviors considered to be tightly linked to ecology in broadly sympatric vervets (Cercopithecus aethiops) and patas monkeys (Erythrocebus patas), two closely related cercopithecines that are exemplars of this dichotomy, with the expectation that vervets would exhibit stronger rank differences than patas monkeys in these behaviors. Overall, rank explained more than twice as much variation among vervets as among patas monkeys in ranging behavior, activity budgets, and diet. Vervets did not, however, exhibit stronger rank differences when they used Acacia xanthophloea habitat, in which foods are more usurpable, compared to Acacia drepanolobium habitat, in which foods are less usurpable. In Acacia drepanolobium habitat, to which patas are restricted, higher-ranking vervets converged in behavior with patas monkeys to a greater extent than lower-ranking vervets, suggesting that social constraints interfere with the foraging efficiency of lower-ranking vervets even in habitats in which there are fewer opportunities to usurp foods. 相似文献
59.
MARK2/EMK1/Par-1Balpha phosphorylation of Rab11-family interacting protein 2 is necessary for the timely establishment of polarity in Madin-Darby canine kidney cells 下载免费PDF全文
Ducharme NA Hales CM Lapierre LA Ham AJ Oztan A Apodaca G Goldenring JR 《Molecular biology of the cell》2006,17(8):3625-3637
Rab11a, myosin Vb, and the Rab11-family interacting protein 2 (FIP2) regulate plasma membrane recycling in epithelial cells. This study sought to characterize more fully Rab11-FIP2 function by identifying kinase activities modifying Rab11-FIP2. We have found that gastric microsomal membrane extracts phosphorylate Rab11-FIP2 on serine 227. We identified the kinase that phosphorylated Rab11-FIP2 as MARK2/EMK1/Par-1Balpha (MARK2), and recombinant MARK2 phosphorylated Rab11-FIP2 only on serine 227. We created stable Madin-Darby canine kidney (MDCK) cell lines expressing enhanced green fluorescent protein-Rab11-FIP2 wild type or a nonphosphorylatable mutant [Rab11-FIP2(S227A)]. Analysis of these cell lines demonstrates a new role for Rab11-FIP2 in addition to that in the plasma membrane recycling system. In calcium switch assays, cells expressing Rab11-FIP2(S227A) showed a defect in the timely reestablishment of p120-containing junctional complexes. However, Rab11-FIP2(S227A) did not affect localization with recycling system components or the normal function of apical recycling and transcytosis pathways. These results indicate that phosphorylation of Rab11-FIP2 on serine 227 by MARK2 regulates an alternative pathway modulating the establishment of epithelial polarity. 相似文献
60.
Small scale variation in decay rate within logs one year after felling: Effect of fungal community structure and moisture content 总被引:2,自引:0,他引:2
Abstract Fungal species composition, moisture content, percentage weight loss, and instantaneous decay rate (expressed by rate of CO2 evolution) was assessed for a total of 186 8 cm3 cubes from 10 beech logs which had been decomposing on the forest floor for 14 months. There was considerable within and between branch variation in decay rate and water content. Water content at the time of sampling was not directly correlated with percentage weight loss or instantaneous decay rate, nor was it correlated with position in the log. However, wood occupied by Ascomycotina (other than Nectria ) tended to be drier than that occupied by Basidiomycotina. In particular wood occupied by Xylaria hypoxylon was drier than that occupied by all other species, although wood in which X. hypoxylon was replacing other fungi was wetter than when X. hypoxylon was alone. Variation in percentage weight loss could not be explained in terms of water content and fungal species composition at the time of sampling, but variation in instantaneous decay rate could. Thus, decay rate by Ascomycotina was significantly less ( P < 0.05) than by Basidiomycotina, and rate of CO2 evolution from wood occupied by X. hypoxylon alone was significantly slower than from wood in which X. hypoxylon was replacing H. fragiforme or Nectria . The latter was partially correlated with water content but whether this is a cause and effect relationship is uncertain. 相似文献