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891.
A method for the rapid and precise determination of acclimated phytoplankton reproduction rates 总被引:9,自引:0,他引:9
Brand Larry E.; Guillard Robert R.L.; Murphy Lynda S. 《Journal of plankton research》1981,3(2):193-201
A rapid method for measuring, simultaneously, the asexual reproductionrates of hundreds of phytoplankton cultures is described. Thismethod is based on the dairy measurement of in vivo chlorophyllfluorescence read directly in the culture tubes. Hundreds ofthese culture tubes, containing specially prepared culture medium,can be maintained in identical environments in specially designedconstant environment devices. The method is capable of measuringthe acclimated reproduction rates of phytoplankton cultureswith an error of 34% (coefficient of variation). Completeacclimation, crucial to the detection of small genetic differencesbetween clones, takes one to three weeks, thus necessitatinglong-term experiments. Studies using the methods described indicatethat, in a constant environment, coccolithophores and dinoflagellatesreproduce at constant rates, but diatoms do not. 相似文献
892.
893.
Bernardine M. Pinto Matthew M. Clark Dean G. Cruess Lynda Szymanski Vincent Pera 《Obesity (Silver Spring, Md.)》1999,7(3):288-292
PINTO, BERNARDINE M., MATTHEW W. CLARK, DEAN G. CRUESS, LYNDA SZYMANSKI, AND VINCENT PERA. Changes in self-efficacy and decisional balance for exercise among obese women in a weight management program. Obes Res. Objective: To assess changes in eating and exercise self-efficacy and decisional balance for exercise in an obese population enrolled in a multi-disciplinary weight management program. Research Method and Procedures: Thirty-two obese women were assessed at entry and after 12 weeks of treatment. Results: Participants showed 15% mean weight loss after 12 weeks of treatment. Mean minutes of self-reported exercise increased by 229%. Participants demonstrated significant improvements in self-efficacy for both eating and exercise and health parameters but not in decisional balance for exercise adoption. Discussion: These results may have implications for the practitioner in that self-efficacy changes occur during successful weight loss, but decisional balance for exercise may not change until individuals enter maintenance. These results imply that practitioners may be advised to shift from confidence building to relapse prevention early on in treatment, and that focusing on strengthening the pros of exercise and reducing the cons of exercise may need to remain constant during treatment. 相似文献
894.
Lynda Latrèche Olivier Jean-Jean Donna M. Driscoll Laurent Chavatte 《Nucleic acids research》2009,37(17):5868-5880
The selenocysteine insertion sequence (SECIS) element directs the translational recoding of UGA as selenocysteine. In eukaryotes, the SECIS is located downstream of the UGA codon in the 3′-UTR of the selenoprotein mRNA. Despite poor sequence conservation, all SECIS elements form a similar stem-loop structure containing a putative kink-turn motif. We functionally characterized the 26 SECIS elements encoded in the human genome. Surprisingly, the SECIS elements displayed a wide range of UGA recoding activities, spanning several 1000-fold in vivo and several 100-fold in vitro. The difference in activity between a representative strong and weak SECIS element was not explained by differential binding affinity of SECIS binding Protein 2, a limiting factor for selenocysteine incorporation. Using chimeric SECIS molecules, we identified the internal loop and helix 2, which flank the kink-turn motif, as critical determinants of UGA recoding activity. The simultaneous presence of a GC base pair in helix 2 and a U in the 5′-side of the internal loop was a statistically significant predictor of weak recoding activity. Thus, the SECIS contains intrinsic information that modulates selenocysteine incorporation efficiency. 相似文献