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排序方式: 共有842条查询结果,搜索用时 31 毫秒
781.
Brigitte Spillmann Lynda P. Dunkel Maria A. Van Noordwijk Rahmalia N. A. Amda Adriano R. Lameira Serge A. Wich Carel P. Van Schaik 《Ethology : formerly Zeitschrift fur Tierpsychologie》2010,116(5):385-395
Long calls by flanged male Bornean orang‐utans (Pongo pygmaeus wurmbii) serve as a long‐distance communication signal in this semi‐solitary species and allow individuals to adjust their ranging behavior. Long calls can be heard up to circa 1 km in dense rainforest. Only flanged males emit them, in various contexts: spontaneously (where no disturbances from the environment are perceived by human observers), when highly aroused by another male’s long call or a falling tree nearby, or right after having pushed over a dead tree themselves. In this study, acoustic analyses of orang‐utan long calls at Tuanan in Central Kalimantan not only confirm the discrimination of individual males by their long calls but also demonstrate the discrimination of context based on the long calls’ acoustic structure, which is further supported by the females’ ranging responses according to long call contexts. Females with dependent offspring move away from spontaneous long call sources but appear to ignore long calls elicited by disturbance. Hence, Bornean orang‐utan females perceive measurable differences in acoustic characteristics of long calls given in different contexts. These findings concur with vocal discrimination of contexts in other non‐human primates. 相似文献
782.
Patrice E. Fort Mandy K. Losiewicz Lynda Elghazi Dejuan Kong Corentin Cras-Mneur Diane C. Fingar Scot R. Kimball Raju V.S. Rajala Alexander J. Smith Robin R. Ali Steven F. Abcouwer Thomas W. Gardner 《The Journal of biological chemistry》2022,298(6)
Mechanistic target of rapamycin (mTOR) and mTOR complex 1 (mTORC1), linchpins of the nutrient sensing and protein synthesis pathways, are present at relatively high levels in the ganglion cell layer (GCL) and retinal ganglion cells (RGCs) of rodent and human retinas. However, the role of mTORCs in the control of protein synthesis in RGC is unknown. Here, we applied the SUrface SEnsing of Translation (SUnSET) method of nascent protein labeling to localize and quantify protein synthesis in the retinas of adult mice. We also used intravitreal injection of an adeno-associated virus 2 vector encoding Cre recombinase in the eyes of mtor- or rptor-floxed mice to conditionally knockout either both mTORCs or only mTORC1, respectively, in cells within the GCL. A novel vector encoding an inactive Cre mutant (CreΔC) served as control. We found that retinal protein synthesis was highest in the GCL, particularly in RGC. Negation of both complexes or only mTORC1 significantly reduced protein synthesis in RGC. In addition, loss of mTORC1 function caused a significant reduction in the pan-RGC marker, RNA-binding protein with multiple splicing, with little decrease of the total number of cells in the RGC layer, even at 25 weeks after adeno-associated virus-Cre injection. These findings reveal that mTORC1 signaling is necessary for maintaining the high rate of protein synthesis in RGCs of adult rodents, but it may not be essential to maintain RGC viability. These findings may also be relevant to understanding the pathophysiology of RGC disorders, including glaucoma, diabetic retinopathy, and optic neuropathies. 相似文献
783.
Multi‐decadal stability of woody cover in a mesic eucalypt savanna in the Australian monsoon tropics
Lynda D. Prior Timothy G. Whiteside Grant J. Williamson Renee E. Bartolo David M. J. S. Bowman 《Austral ecology》2020,45(5):621-635
Previous analyses of historical aerial photography and satellite imagery have shown thickening of woody cover in Australian tropical savannas, despite increasing fire frequency. The thickening has been attributed to increasing precipitation and atmospheric CO2 enrichment. These analyses involved labour‐intensive, manual classification of vegetation, and hence were limited in the extent of the areas and the number of measurement times used. Object‐based, semi‐automated classification of historical sequences of aerial photography and satellite imagery has enabled the spatio‐temporal analysis of woody cover over entire landscapes, thus facilitating measurement, monitoring and attribution of drivers of change. Using this approach, we investigated woody cover change in 4000 ha of intact mesic savanna in the Ranger uranium lease and surrounding Kakadu National Park, using imagery acquired on 10 occasions between 1950 and 2016. Unlike previous studies, we detected no overall trend in woody cover through time. Some variation in cover was related to rainfall in the previous 12 months, and there were weak effects of fire in the year of image acquisition and the antecedent 4 years. Our local‐scale study showed a mesic eucalypt savanna in northern Australia has been resilient to short‐term variation in rainfall and fire activity; however, changes in canopy cover could have occurred in other settings. When applying this semi‐automated approach to similar studies of savanna dynamics, we recommend maximising the time depth and number of measurement years, standardising the time of year for image acquisition and using many plots of 1 ha in area, rather than fewer, larger plots. 相似文献
784.
785.
McDowell LM Frazier BA Studelska DR Giljum K Chen J Liu J Yu K Ornitz DM Zhang L 《The Journal of biological chemistry》2006,281(11):6924-6930
Most Apert syndrome patients harbor a single amino acid mutation (S252W) in fibroblast growth factor (FGF) receptor 2 (FGFR2), which leads to abnormal FGF/FGFR2 signaling. Here we show that specific combinations of FGFs and glycosaminoglycans activate both alternative splice forms of the mutant but not of the wild-type FGF receptors. More importantly, 2-O- and N-sulfated heparan sulfate, prepared by a combined chemical and enzymatic synthesis, antagonized the over-activated FGFR2b (S252W) to basal levels at nanomolar concentrations. These studies demonstrated that specific glycosaminoglycans could be useful in treating ligand-dependent FGFR signaling-related diseases, such as Apert syndrome and cancer. 相似文献
786.
Peterson LJ Rajfur Z Maddox AS Freel CD Chen Y Edlund M Otey C Burridge K 《Molecular biology of the cell》2004,15(7):3497-3508
To study the dynamics of stress fiber components in cultured fibroblasts, we expressed alpha-actinin and the myosin II regulatory myosin light chain (MLC) as fusion proteins with green fluorescent protein. Myosin activation was stimulated by treatment with calyculin A, a serine/threonine phosphatase inhibitor that elevates MLC phosphorylation, or with LPA, another agent that ultimately stimulates phosphorylation of MLC via a RhoA-mediated pathway. The resulting contraction caused stress fiber shortening and allowed observation of changes in the spacing of stress fiber components. We have observed that stress fibers, unlike muscle myofibrils, do not contract uniformly along their lengths. Although peripheral regions shortened, more central regions stretched. We detected higher levels of MLC and phosphorylated MLC in the peripheral region of stress fibers. Fluorescence recovery after photobleaching revealed more rapid exchange of myosin and alpha-actinin in the middle of stress fibers, compared with the periphery. Surprisingly, the widths of the myosin and alpha-actinin bands in stress fibers also varied in different regions. In the periphery, the banding patterns for both proteins were shorter, whereas in central regions, where stretching occurred, the bands were wider. 相似文献
787.
Where to from here? The mechanisms enabling the movement of first instar caterpillars on whole plants using Helicoverpa armigera (Hübner) 总被引:1,自引:0,他引:1
Lynda E. Perkins Bronwen W. Cribb Jim Hanan Ezekiel Glaze Christine Beveridge Myron P. Zalucki 《Arthropod-Plant Interactions》2008,2(4):197-207
Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) is an economically-important, polyphagous herbivore in Old World countries. The distribution
of larvae within various host plants has been described, but few studies have tried to determine the behavioural mechanisms
by which the given distributions arose. Our aim was to determine the mechanisms which enable larval movement on pea plants,
starting with first instars. Observations and bioassays determined larval movement in response to light and angled surfaces,
as well as the effect of feeding and plant volatiles on these responses. The majority (68–72%) of 1st instars were positively
phototactic towards blue, green and white light and 42% towards UV light. In the dark, larvae showed negative geotaxis. The
angle of their substrate also had a kinetic effect on larvae; the steeper the angle from horizontal the more larvae moved
under all conditions. Phenylacetaldehyde (a flower volatile) suppressed larval movement except at 90°. (Z)-3-Hexenyl acetate (a green leaf volatile) reversed the direction of movement at the flattest angle. Feeding lessened the
probability of moving. We suggest that phototaxis and geotaxis are behaviours common to larval lepidopterans (caterpillars),
and that these basic behaviours are modulated by environmental, larval, and plant factors to give observed distributions.
Using a multinomial model approach, we created a flow chart to qualitatively and quantitatively represent the decision-making
process of first instar H. armigera in response to the factors influencing movement. 相似文献
788.
789.
790.
Patricia T. Sokol William Hu Lynda Yi Joyce Toral Manik Chandra M. Reza Ziai 《Journal of Protein Chemistry》1994,13(1):117-128
The receptor for the bee venom derived neurotoxin, apamin, is widely believed to be an integral component of the small conductance calcium-activated potassium channel in many excitable cells. By affinity chromatography on immobilized apamin, a 78 kD apamin binding protein of the bovine brain synaptosomes was isolated. Antibodies were elicited against this protein and used to clone a cDNA from a porcine vascular smooth muscle expression library. This gene (Kcal 1.8) codes for a 438 amino protein with four potential transmembrane domains, one putative calcium binding site, a protein kinase C phosphorylation site, and a leucine zipper motif. Kcal 1.8 encoded protein has no significant sequence homologies with any known ion channels or receptors. Kcal 1.8 is likely to encode a protein associated with the small conductance calcium-activated potassium channel in vascular smooth muscle. 相似文献