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951.
Jun Yan Xuefei Cai Jianghong Luo Shusei Sato Qunyi Jiang Jun Yang Xiangling Cao Xiaohe Hu Satoshi Tabata Peter M. Gresshoff Da Luo 《Plant physiology》2010,152(2):797-807
The endogenous trans-acting small interfering RNA (ta-siRNA) pathway plays a conserved role in adaxial-abaxial patterning of lateral organs in simple-leafed plant species. However, its function in compound-leafed species is largely unknown. Using the compound-leafed species Lotus japonicus, we identified and characterized two independent mutants, reduced leaflet1 (rel1) and rel3, whose most conspicuous defects in compound leaves are abaxialized leaflets and reduction in leaflet number. Concurrent mutations in REL genes also compromise flower development and result in radial symmetric floral organs. Positional cloning revealed that REL1 and REL3 encode the homologs of Arabidopsis (Arabidopsis thaliana) SUPPRESSOR OF GENE SILENCING3 and ARGONAUTE7/ZIPPY, respectively, which are key components of the ta-siRNA pathway. These observations, together with the expression and functional data, demonstrated that the ta-siRNA pathway plays conserved yet distinct roles in the control of compound leaf and flower development in L. japonicus. Moreover, the phenotypic alterations of lateral organs in ta-siRNA-deficient mutants and the regulation of downstream targets by the ta-siRNA pathway in L. japonicus were similar to those in the monocots but different from Arabidopsis, indicating many parallels between L. japonicus and the monocots in the control of lateral organ development by the ta-siRNA pathway.Plant endogenous small RNAs can be categorized into microRNAs (miRNAs) and small interfering RNAs (siRNAs) according to their mechanism of biogenesis (Vaucheret, 2006). trans-Acting siRNAs (ta-siRNAs) are one type of siRNA, and their biogenesis requires several key components, such as SUPPRESSOR OF GENE SILENCING3 (SGS3), RNA-DEPENDENT RNA POLYMERASE6 (RDR6), DICER-LIKE4 (DCL4), ARGONAUTE7 (AGO7)/ZIPPY (ZIP), and dsRNA-BINDING4 (Peragine et al., 2004; Vazquez et al., 2004; Gasciolli et al., 2005; Xie et al., 2005; Yoshikawa et al., 2005; Adenot et al., 2006; Nakazawa et al., 2007). Recent studies revealed that the ta-siRNA pathway is integrated into different processes of plant development, such as vegetative phase transition in Arabidopsis (Arabidopsis thaliana; Hunter et al., 2003; Peragine et al., 2004; Xie et al., 2005; Nakazawa et al., 2007) and shoot apical meristem (SAM) initiation in rice (Oryza sativa; Satoh et al., 1999; Itoh et al., 2000; Nagasaki et al., 2007). Parallel studies of this pathway in simple-leafed species also showed that the ta-siRNA pathway plays critical roles in patterning of leaves and floral organs.In flowering plants, leaves and flowers are produced on the periphery of the apical meristem. These lateral organs are structurally asymmetric with regard to the apical meristem. The adaxial side is adjacent to the meristem, while the abaxial side is away from the meristem. The ta-siRNA pathway was found to play a conserved role in specifying the adaxial identity of lateral organs in both monocots and dicots, but defects in the ta-siRNA pathway caused more severe phenotypes in monocots than in dicot Arabidopsis. In Arabidopsis, no clear leaf polarity defects were detected in the ta-siRNA-defective mutants. However, blocking the ta-siRNA pathway in asymmetric1 (as1) or as2 background, which are regulators of leaf adaxial identity (Lin et al., 2003; Xu et al., 2003), results in enhanced adaxial-abaxial leaf defects (Li et al., 2005; Xu et al., 2006; Garcia et al., 2006). In addition, the as2rdr6 double mutants also display aberrant flowers with sepals failing to enwrap the inner whorl organs and some sepals and petals becoming needle-like structures (Li et al., 2005). In maize (Zea mays), mutations in LEAFBLADELESS1 (LBL1), which encodes the Arabidopsis SGS3 ortholog, give rise to abnormal leaves with partial or complete loss of adaxial cell identity (Timmermans et al., 1998; Nogueira et al., 2007). In severe lbl1 mutants, leaf-like lateral organs of inflorescences and flowers develop as symmetric, thread-like organs, and the immature ear is exposed and arrested in development (Timmermans et al., 1998). In rice, the osdcl4-1 mutants display an abaxialized epidermis in coleoptiles and in the first leaf, and knockdown of OsDCL4 can lead to the awn-like lemma with a radial abaxialized identity and the stamens and carpel not enwrapped by the lemma and pelea (Liu et al., 2007). Transgenic rice plants with ectopic expression of SHOOTLESS4 (SHL4), the homolog of Arabidopsis AGO7, exhibit partially adaxialized leaves (Nagasaki et al., 2007; Shi et al., 2007).In addition to the ta-siRNA pathway, other components have also been shown to be involved in the adaxial-abaxial patterning of lateral organs. The Antirrhinum majus PHANTASTICA (PHAN) gene (Waites et al., 1998; Byrne et al., 2000; Xu et al., 2003; Qi et al., 2004), which is the ortholog of Arabidopsis AS1, and CLASS III HOMEODOMAIN-LEUCINE ZIPPER (HD-ZIP III) gene family members (McConnell et al., 2001; Emery et al., 2003) contribute to adaxial pattern formation of lateral organs, whereas members of YABBY (YAB; Sawa et al., 1999; Siegfried et al., 1999) and KANADI (Eshed et al., 2001; Kerstetter et al., 2001) gene families, AUXIN RESPONSE FACTOR3 (ARF3) and ARF4 (Pekker et al., 2005), and the miRNAs miR165/166 (Emery et al., 2003; Eshed et al., 2004; Mallory et al., 2004) are required for specifying abaxial identity. How the activities of these adaxial and abaxial determinants are coordinated has been extensively studied. It was found that ARF3 and ARF4 are regulated by the TAS3 ta-siRNA, and this regulation is conserved in both monocots and dicots (Allen et al., 2005; Williams et al., 2005). Recent studies in Arabidopsis suggest that ta-siRNAs act in a non-cell-autonomous manner to spatially restrict ARF activity (Chitwood et al., 2009; Schwab et al., 2009).In contrast to simple leaves with their single lamina, compound leaves are composed of one petiole and several leaflets. It is found that genes required for the adaxial-abaxial patterning of lateral organs in simple-leafed species also play critical roles in compound-leafed species, but these genes play multiple roles in compound leaf development. In tomato (Solanum lycopersicum), down-regulation of PHAN ortholog disturbs the leaf polarity as well as leaflet formation (Kim et al., 2003). Extensive studies of the PHAN expression in diverse compound-leafed species suggest that the function of PHAN in maintaining leaf adaxial identity is associated with leaflet formation in compound leaves and reduced adaxial identity of leaf primordia by down-regulation of PHAN could change pinnate compound leaves into palmate leaves (Kim et al., 2003). In pea (Pisum sativum), the role of PHAN in compound leaf development has also been elucidated by characterization of the phan mutant crispa (cri; Tattersall et al., 2005). However, unlike antisense PHAN transgenic tomato leaves, the cri mutant has the individual leaflet abaxialized, rather than the whole leaf. The number of lateral organs on the cri mutant compound leaves, including leaflets, is not altered, and the leaves remain pinnate. Apart from leaf development, the cri mutation also affects flower development. Although the floral organ identity and organ number are not altered, the laminar floral organ display abaxialized identity (Tattersall et al., 2005).The ta-siRNA pathway plays a critical role in simple-leafed species, but its role in compound-leafed species is not understood. Here, we address this question by analyzing loss-of-function reduced leaflet (rel1) and rel3 mutants in the compound-leafed species Lotus japonicus. Phenotypic characterization shows compound leaves of rel mutants exhibit a conspicuous disturbance in leaflet polarity as well as reduction in leaflet number. Besides the abnormal compound leaves, flower development is also severely affected in rel mutants, showing radial symmetric petals. REL1 and REL3 were identified by map-based cloning and were shown to be homologs of Arabidopsis SGS3 and AGO7, respectively. REL1 and REL3 act in the same genetic pathway and are both required for the biogenesis of TAS3 ta-siRNA. Further investigation reveals that the homolog of the Arabidopsis ARF3 is duplicated in the L. japonicus genome and that the duplicate ARF3 homologs and the ARF4 homolog are all negatively regulated by the ta-siRNA pathway. Furthermore, we found that the expression of LjYAB1, a homolog of Arabidopsis YAB1, was decreased in rel mutants, which may be associated with the reduced lamina.Taken together, our data reveal that the ta-siRNA pathway is integrated into the regulatory networks in the control of lateral organ development in L. japonicus and further emphasize the importance of the ta-siRNA pathway in compound leaf development. Moreover, our results also indicate many parallels between L. japonicus and monocots for the ta-siRNA pathway in the regulation of lateral organs. 相似文献
952.
Yan Li Yunshan Ning Yundan Wang Dandan Peng Yaodong Jiang Lili Zhang Min Long Jun Luo Ming Li 《BMC biotechnology》2010,10(1):84
Background
Urease B is an important virulence factor that is required for Helicobacter pylori to colonise the gastric mucosa. Mouse monoclonal antibodies (mAbs) that inhibit urease B enzymatic activity will be useful as vaccines for the prevention and treatment of H. pylori infection. Here, we produced murine mAbs against urease B that neutralize the enzyme's activity. We mapped their epitopes by phage display libraries and investigated the immunogenicity of the selected mimotopes in vivo. 相似文献953.
Teng Y Luo Y Sun X Tu C Xu L Liu W Li Z Christie P 《International journal of phytoremediation》2010,12(5):516-533
A field experiment was conducted to study the effects of inoculation with the arbuscular mycorrhizal fungus Glomus caledonium and/or Rhizobium meliloti on phytoremediation of an agricultural soil contaminated with weathered PCBs by alfalfa grown for 180 days. Planting alfalfa (P), alfalfa inoculated with G. caledonium (P + AM), alfalfa inoculated with R. meliloti (P + R), and alfalfa co-inoculated with R. meliloti and G. caledonium (P+AM+R) decreased significantly initial soil PCB concentrations by 8.1, 12.0, 33.8, and 43.5%, respectively. Inoculation with R. meliloti and/or G. caledonium (P+AM+R) increased the yield of alfalfa, and the accumulation of PCBs in the shoots. Soil microbial counts and the carbon utilization ability of the soil microbial community increased when alfalfa was inoculated with R. meliloti and/or G. caledonium. Results of this field study suggest that synergistic interactions between AMF and Rhizobium may have great potential to enhance phytoremediation by alfalfa of an agricultural soil contaminated with weathered PCBs. 相似文献
954.
Fungi in the basidiomycetous genus Amanita owe their high mammalian toxicity to the bicyclic octapeptide amatoxins such as α-amanitin. Amatoxins and the related phallotoxins (such as the heptapeptide phalloidin) are encoded by members of the "MSDIN" gene family and are synthesized on ribosomes as short (34- to 35-amino-acid) proproteins. Antiamanitin antibodies and confocal microscopy were used to determine the cellular and subcellular localizations of amanitin accumulation in basidiocarps (mushrooms) of the Eastern North American destroying angel (Amanita bisporigera). Consistent with previous studies, amanitin is present throughout the basidiocarp (stipe, pileus, lamellae, trama, and universal veil), but it is present in only a subset of cells within these tissues. Restriction of amanitin to certain cells is especially marked in the hymenium. Several lines of evidence implicate a specific prolyl oligopeptidase, A. bisporigera POPB (AbPOPB), in the initial processing of the amanitin and phallotoxin proproteins. The gene for AbPOPB is restricted taxonomically to the amatoxin-producing species of Amanita and is clustered in the genome with at least one expressed member of the MSDIN gene family. Immunologically, amanitin and AbPOPB show a high degree of colocalization, indicating that toxin biosynthesis and accumulation occur in the same cells and possibly in the same subcellular compartments. 相似文献
955.
Identification of the FANCI protein, a monoubiquitinated FANCD2 paralog required for DNA repair 总被引:10,自引:0,他引:10
Smogorzewska A Matsuoka S Vinciguerra P McDonald ER Hurov KE Luo J Ballif BA Gygi SP Hofmann K D'Andrea AD Elledge SJ 《Cell》2007,129(2):289-301
Fanconi anemia (FA) is a developmental and cancer-predisposition syndrome caused by mutations in genes controlling DNA interstrand crosslink repair. Several FA proteins form a ubiquitin ligase that controls monoubiquitination of the FANCD2 protein in an ATR-dependent manner. Here we describe the FA protein FANCI, identified as an ATM/ATR kinase substrate required for resistance to mitomycin C. FANCI shares sequence similarity with FANCD2, likely evolving from a common ancestral gene. The FANCI protein associates with FANCD2 and, together, as the FANCI-FANCD2 (ID) complex, localize to chromatin in response to DNA damage. Like FANCD2, FANCI is monoubiquitinated and unexpectedly, ubiquitination of each protein is important for the maintenance of ubiquitin on the other, indicating the existence of a dual ubiquitin-locking mechanism required for ID complex function. Mutation in FANCI is responsible for loss of a functional FA pathway in a patient with Fanconi anemia complementation group I. 相似文献
956.
Non-oncogene addiction and the stress phenotype of cancer cells 总被引:1,自引:0,他引:1
957.
p31comet blocks Mad2 activation through structural mimicry 总被引:2,自引:0,他引:2
The status of spindle checkpoint signaling depends on the balance of two opposing dynamic processes that regulate the highly unusual two-state behavior of Mad2. In mitosis, a Mad1-Mad2 core complex recruits cytosolic Mad2 to kinetochores through Mad2 dimerization and converts Mad2 to a conformer amenable to Cdc20 binding, thereby facilitating checkpoint activation. p31(comet) inactivates the checkpoint through binding to Mad1- or Cdc20-bound Mad2, thereby preventing Mad2 activation and promoting the dissociation of the Mad2-Cdc20 complex. Here, we report the crystal structure of the Mad2-p31(comet) complex. The C-terminal region of Mad2 that undergoes rearrangement in different Mad2 conformers is a major structural determinant for p31(comet) binding, explaining the specificity of p31(comet) toward Mad1- or Cdc20-bound Mad2. p31(comet) adopts a fold strikingly similar to that of Mad2 and binds at the dimerization interface of Mad2. Thus, p31(comet) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2." 相似文献
958.
Manuzon MY Hanna SE Luo H Yu Z Harper WJ Wang HH 《Applied and environmental microbiology》2007,73(5):1676-1677
A TaqMan real-time PCR assay was developed to quantify the tetS gene pool present in retail cheeses. This protocol offers a rapid, specific, sensitive, and culture-independent method for assessing antibiotic resistance genes in food samples rich in fats and proteins. 相似文献
959.
Qihai Zhou Fuwen Wei Chengming Huang Ming Li Baoping Ren Bang Luo 《International journal of primatology》2007,28(3):657-671
Activity patterns and time budgets are 2 important aspects of animal behavior that researchers use to investigate ecological influences on individual behavior. We collected data on activity patterns and time budgets in 1 group of François’ langurs (Trachypithecus francoisi) from August 2003 to July 2004 in the Nonggang Nature Reserve, Guangxi Province, China, via instantaneous scan sampling method with 15-min intervals. The diurnal activity pattern of François’ langurs showed morning and afternoon feeding peaks, with a midday resting peak. Seasonal change was apparent in the activity pattern: 2 significant feeding peaks occurred in the dry season and only 1 significant feeding peak in the rainy season. The group spent an average of 51.5% of the daytime resting. Feeding and moving accounted on average for 23.1% and 17.3% of the activity budget, respectively. Subjects spent little time on social activities, averaging 2% for grooming and 5.5% for playing. Their time budgets showed significant seasonal variation: they spent a greater proportion of time on feeding and less time on resting and grooming in the dry season than in the rainy season. They also differed among different sex-age classes: immatures spent more time playing, whereas adults devoted more time to resting, feeding, and grooming. Correlations between time budgets and food items or food availability clearly indicated that François’ langurs might adopt an energy-maximizing strategy when preferred foods were scarce in the dry season. 相似文献
960.
Hui Song Qun-Feng Lou Xiang-Dong Luo Joseph N. Wolukau Wei-Ping Diao Chun-Tao Qian Jin-Feng Chen 《Plant Cell, Tissue and Organ Culture》2007,90(3):245-254
Six experiments (including pretreatment, embryonic callus induction media, preculture conditions, embryo induction media,
embryo germination media, and genotypic effects) were conducted to develop an efficient cucumber (Cucumis sativus L., 2n = 2x = 14) anther culture protocol. Pretreatment and embryo induction were key factors for successful anther culture. Suitable
temperature stress depended on the ecotype, i.e., cucumbers from cold areas responded well to cold shock whereas those from
temperate areas responded well to heat treatment. The best medium for embryonic callus induction was MS medium supplemented
with 4.44 μM BA, 2.26 μM 2, 4-D, 4.64 μM KIN, 3% sucrose and 0.8% agar. For embryo induction, MS medium supplemented with
0.54 μM NAA, 13.32 μM BA, 3% sucrose and 0.8% agar was optimal, and for embryo germination MS medium containing 2.22 μM BA,
6% sucrose and 1.2% agar was best. Using this protocol, we produced callus from 16 genotypes and regenerated plants from three
of 20 evaluated. Three embryos per anther and 42 DH per 45 anthers (93% success) were obtained for cv. Ningjia No. 1, which
was an improved result over a previous report. The origin of regenerants from microspores was determined by cytological, morphological
and AFLP analyses. 相似文献