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101.
The protein conformation and a zinc-binding domain of an autoantigen from mouse seminal vesicle.
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The protein conformation of a mouse seminal vesicle autoantigen was studied by circular dichroism spectroscopy. At pH 7.4, the spectrum in the UV region appears as one negative band at 217 nm and one positive band at 200 nm. This together with the predicted secondary structures indicates no helices but a mixture of beta form, beta turn, and unordered form in the protein molecule. The conformation is stable even at pH 10.5 or 3.0. The spectrum in the near-UV region consists of fine structures that are disturbed in acidic or alkaline solution. The environments around Trp2 and Trp82 of this protein were studied by intrinsic fluorescence and solute quenching. They give an emission peak at 345 nm, and about 87% of them are accessible to quenching by acrylamide. Correlating the quenching effect of CsCl and Kl on the protein fluorescence to the charged groups along the polypeptide chain suggests the difference in the "local charge" around the two tryptophan residues. The presence of ZnCl2 in the protein solution effects no change in the circular dichroism but perturbs the fluorescence due to Trp82. Analysis of the fluorescence data suggests a Zn(2+)-binding site on the protein, which cannot coordinate with both Ca2+ and Mg2+. The association constant for the complex formation is 1.35 x 10(5) +/- 0.04 x 10(5) M-1 at pH 7.4. 相似文献
102.
Infectivity enhancement by human immunodeficiency virus type 1 Nef is independent of its association with a cellular serine/threonine kinase. 总被引:2,自引:2,他引:0
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Nef proteins from human immunodeficiency virus type 1 isolate SF2 (HIV-1SF2) and simian immunodeficiency virus isolate mac239 (SIVmac239) have been found to associate with a cellular serine/threonine kinase designated NAK. We have recently shown that the association of Nef with NAK is isolate dependent. To identify the structural basis for Nef-kinase association, several chimeric molecules were constructed between SF2 Nef (binding NAK) and 233 Nef (a primary isolate not binding NAK) and stably expressed in HuT-78 human T cells via retrovirus-mediated gene transfer. The Nef 233/SF2/SF2 chimera in which the N-terminal 37 amino acids of SF2 Nef were replaced by those of 233 Nef showed the same ability as SF2 Nef to bind NAK. The Nef 233/SF2/233 chimera in which the N-terminal 37 amino acids and the C-terminal 72 amino acids of SF2 Nef were replaced by corresponding sequences from 233 Nef completely lost the ability to associate with the kinase activity. Furthermore, replacement of the C-terminal 72 amino acids of 233 Nef with the equivalent SF2 sequence (chimera 233/233/SF2) fully restored kinase association to 233 Nef. These results suggest that (i) the core of Nef is not sufficient for NAK binding, (ii) the C terminus of SF2 Nef contains structural determinants important for association with NAK, and (iii) the failure of 233 Nef to bind NAK is due to a defect in its C terminus. Taking advantage of the C terminus of 233 Nef being nonfunctional and using an infectious clone of HIV-1SF2, we show that association with NAK is not required for Nef-mediated infectivity enhancement. While the strong and reproducible association of some Nef isolates with NAK has been clearly established, the role of NAK in Nef function remains to be fully elucidated. 相似文献
103.
Theiler's murine encephalomyelitis viruses (TMEVs) belong to the Picornaviridae family and are divided into two groups, typified by strain GDVII virus and members of the TO (Theiler's original) group. The highly virulent GDVII group causes acute encephalitis in mice, while the TO group is less virulent and causes a chronic demyelinating disease which is associated with viral persistence in mice. This persistent central nervous system infection with demyelination resembles multiple sclerosis (MS) in humans and has thus become an important model for studying MS. It has been shown that some of the determinants associated with viral persistence are located on the capsid proteins of the TO group. Structural comparisons of two persistent strains (BeAn and DA) and a highly virulent strain (GDVII) showed that the most significant structural variations between these two groups of viruses are located on the sites that may influence virus binding to cellular receptors. Most animal viruses attach to specific cellular receptors that, in part, determine host range and tissue tropism. In this study, atomic models of TMEV chimeras were built with the known structures of GDVII, BeAn, and DA viruses. Comparisons among the known GDVII, BeAn, and DA structures as well as the predicted models for the TMEV chimeras suggested that a gap on the capsid surface next to the putative receptor binding site, composed of residues from VP1 and VP2, may be important in determining viral persistence by influencing virus attachment to cellular receptors, such as sialyloligosaccharides. Our results showed that sialyllactose, the first three sugar molecules of common oligosaccharides on the surface of mammalian cells, inhibits virus binding to the host cell and infection with the persistent BeAn virus but not the nonpersistent GDVII and chimera 39 viruses. 相似文献
104.
广西九万山藓类植物区系分析及其对划分热带,亚热带分界线的意义 总被引:13,自引:0,他引:13
九万山位于广西北部的中央(25°10’~25°25’N,108°27’E),界于泛北极植物区和古热带植物区的交汇处。其主要植被为亚热带常绿阔叶林。最高海拔为1693m,最低处仅170m。经对1850号标本的鉴定,共计有藓类植物35科、101属和189种。本文分析了九万山的藓类植物区系成分,将其划分为 10种类型。其中东亚成分最为丰富(39.33%),热带、亚热带成分次之(38.20%),而温带成分居第三位(18.54%)。本文选择了九万山临近的五个地区加以比较。其中,金佛山与九万山藓类植物的属和种的相似性系数分别为60.68%和36.87%,神农架为57.29%和33.13%。尖峰岭为48.83%和22.29%,西双版纳为57.29%和29.63%。而我国东部的武夷山则为69.86%和39.57%,其相似性系数在相比较的五个山区中为最高。九万山区有4个特有属并明显受喜马拉雅的影响。特别值得注意的是,在九万山区藓类区系中约有7%的种类是典型的热带成分。根据定量和定性的分析,笔者认为九万山的藓类区系表现出由热带向亚热带过渡的特性,其分界线可能位于九万山的南侧。 相似文献
105.
干燥番茄(Lycopersicon esculentum Mill. cv. Moneym aker)种子浸种吸水有明显的3 个阶段,尽管种子不同部分吸水的速度不一样,但整粒种子需10~12 h 才能基本完成第一阶段的吸水。胚乳对种胚伸长和生长的机械压力明显阻碍种胚的吸水,即使在第二阶段整个种子水势与浸种溶液基本达到水分平衡时,种胚的水势仍然低于整个种子水势0.6~0.9 MPa。发芽过程中GA 和ABA不直接影响番茄种子吸水。番茄种胚水势与含水量的关系呈幂指数的正相关。无论在浸种还是渗控处理过程中,番茄种胚压力势的总变化趋势是下降的 相似文献
106.
四川55种鱼生活史型的研究 总被引:1,自引:0,他引:1
四川55种鱼生活史型的研究刁晓明,罗一兵李波(西南农业大学水产系,重庆630716)(重庆大学计算机系,630000)LifeHistoryPatternsof55FishSpeciesinSichuan¥DiaoXiaoming;LuoYibing... 相似文献
107.
108.
<正> 白细胞介素-2(Interleukin-2,IL-2)是一种重要的免疫调节剂,具有促进T细胞增殖和分化,调节NK细胞活性,诱导LAK等功能,在肿瘤治疗上有广泛的应用前景。我们的前文就重组菌中IL-2包含体的制备、性质及重组rIL-2的纯化进行了研究,本文就重组rIL-2纯化过程中影响其活性的有关因素进行了探讨。 相似文献
109.
Functional domains of the Escherichia coli F plasmid oriT locus were identified by deletion analysis. DNA sequences required for nicking or transfer were revealed by cloning deleted segments of oriT into otherwise nonmobilizable pUC8 vectors and testing for their ability to promote transfer or to be nicked when tra operon functions were provided in trans. Removal of DNA sequences to the right of the central A + T-rich region (i.e., from the direction of traM) did not affect the susceptibility of oriT to nicking functions; however, transfer efficiency for oriT segments deleted from the right was progressively reduced over an 80- to 100-bp interval. Deletions extending toward the oriT nick site from the left did not affect the frequency of transfer if deletion endpoints lay at least 22 bp away from the nick site. Deletions or insertions in the central, A + T-rich region caused periodic variation in transfer efficiency, indicating that phase relationships between nicking and transfer domains of oriT must be preserved for full oriT function. These data show that the F oriT locus is extensive, with domains that individually contribute to transfer, nicking, and overall structure. 相似文献
110.
本文记述金小蜂科柄腹金小蜂亚科蝽卵金小蜂属一新种,正模、配模、副模,采自北京市平谷县。 相似文献