Endometrial ultrastructure in the early uterine response to blastocysts and artificial deciduogenic stimuli in rats

Summary The early uterine response to transplanted, delayed and estrogenactivated blastocysts was studied ultrastructurally and compared with that induced by intrauterine instillations of deciduogenic agents (arachis oil, air). The uterine responses to delayed and activated blastocysts showed no ultrastructural or temporal differences. Already within 4 h after transfer to a sensitized uterus, the delayed blastocysts exhibited signs of activation, and both types of blastocysts had started to attach onto an undamaged epithelial lining. Signs of stromal cell differentiation into decidual cells were also seen as early as 4 h after transfer, while the Pontamine-blue reaction did not appear until after 8 h. The results therefore indicate that the transplanted blastocysts induced decidualization atraumatically and that the delayed blastocysts were either deciduogenic already before transfer or rapidly acquired deciduogenic properties after transfer.Artificial decidual induction with oil and air led to damage or death of a large number of cells in the uterine luminal epithelium. Within only 15 min after instillation pronounced signs of cell damage were seen, and later numerous cells were extruded from the epithelial lining. In the stroma ultrastructural signs of decidual cell differentiation and a Pontamine-blue reaction were observed as early as 4 h after induction. It is therefore suggested that oil and air induce decidualization via the epithelium by means of trauma.Supported by grants from the Swedish Medical Research Council (Project 12X-70)     

A technique for determining individual fresh weights of live small animals,with special reference to Enchytraeidae

Summary A technique is presented in which desiccation as a source of error in fresh weight determinations of soft-bodied microfauna is avoided by weighing the animal in a drop of silica fluid.