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991.
The kinetics of the changes in the cytoplasmic Ca2+ concentration (Cai2+) and amylase release were measured in fura-2-loaded pancreatic acinar cells and perifused pancreatic acini, respectively. Cholecystokinin octapeptide (CCK-8) and its amphibian analogue caerulein induced similar dose-related increases of Cai2+ and amylase secretion with threshold concentrations of 2–6·10−12 M, and maximal effects at 2·10−10 M. The action of CCK/caerulein on Cai2+ was complex and similar to that of carbachol and bombesin with a prompt several-fold increase within seconds followed by a gradual decline over more than 5 min to a new sustained suprabasal level. The kinetics of amylase release in response to CCK and carbachol correlated with the changes in Cai2+. Additions of the antagonists N2,O2-dibutyrylguanosine 3′:5′-cyclic monophosphate and atropine after 30 min of CCK-8 and carbachol stimulation, respectively, were associated with prompt lowerings of Cai2+ and inhibitions of amylase secretion. The patterns observed with substance P (SP) and eledoisin were different with high concentrations (10−8–10−7 M) giving monophasic increases of Cai2+ and amylase release. An initial stimulation of cells with a high dose of CCK eliminated the Cai2+ response to further stimulation with CCK, carbachol, bombesin and SP, whereas cells subjected to initial stimulation with SP responded to subsequent exposure to CCK with prolonged elevation of Cai2+. The data indicate that stimulation with CCK, carbachol and bombesin may be associated with intracellular mobilization of calcium from more than one pool, and that an increase of Cai2+ is involved even in threshold stimulation of amylase release. 相似文献
992.
993.
Predation on hatchery-reared and wild smolts of Atlantic salmon, Salmo salar L., in the estuary of River Orkla, Norway 总被引:1,自引:0,他引:1
Predation on wild and hatchery-reared Atlantic salmon smolts was studied in the estuary of River Orkla. Cod and saithe congregating in the estuary were the most serious predators on smolts. There was no difference between the mortality rates of wild and hatchery-reared smolts. Predation by cod was estimated at 20%. No evidence was found to indicate selective predation on the smallest wild and hatchery-reared smolts. 相似文献
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995.
Vladimir Vincek Dagmar Klein Felipe Figueroa Vera Hauptfeld Masanori Kasahara Colm O'hUigin Bernard Mach Jan Klein 《Immunogenetics》1992,35(4):263-271
The human HLA-DR3 haplotype consists of two functional genes (DRB1*03 and DRB3*01) and one pseudogene (DRB2), arranged in the order DRB1... DRB2... DRB3 on the chromosome. To shed light on the origin of the haplotype, we sequenced 1480 nucleotides of the HLA-DRB2 gene and aong stretches of two other genes, Gogo-DRB2 from a gorilla, Sylvia and Patr-DRB2 from a chimpanzee, Hugo. All three sequences (HLA-DRB2, Gogo-DRB2, Patr-DRB2) are pseudogenes. The HLA-DRB2 and Gogo-DRB2 pseudogenes lack exon 2 and contain a twenty-nucleotide deletion in exon 3, which destroys the correct translational reading frame and obliterates the highly conserved cysteine residue at position 173. The Patr-DRB2 pseudogene lacks exons 1 and 2; it does not contain the twenty-nucleotide deletion, but does contain a characteristic duplication of that part of exon 6 which codes for the last four amino acid residues of the cytoplasmic region. When the nucleotide sequences of these three genes are compared to those of all other known DRB genes, the HLA-DRB2 is seen as most closely related to Gogo-DRB2, indicating orthologous relationship between the two sequences. The Patr-DRB2 gene is more distantly related to these two DRB2 genes and whether it is orthologous to them is uncertain. The three genes are in turn most closely related to HLA-DRBVI (the pseudogene of the DR2 haplotype) and Patr-DRB6 (another pseudogene of the Hugo haplotype), followed by HLA-DRB4 (the functional but nonpolymorphic gene of the DR4 haplotype). These relationships suggest that these six genes evolved from a common ancestor which existed before the separation of the human, gorilla, and chimpanzee lineages. The DRB2 and DRB6 have apparently been pseudogenes for at least six million years (myr). In the human and the gorilla haplotype, the DRB2 pseudogene is flanked on each side by what appear to be related genes. Apparently, the DR3 haplotype has existed in its present form for more than six myr.The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession number M86691–94. 相似文献
996.
Hexanoic acid and octanoic acid inhibited growth of 10 strains of Listeria monocytogenes and two strains of L. innocua at pH 5·0 and pH 5·5 and 20°C. Octanoic acid was more inhibitory than hexanoic acid and both were more inhibitory at pH 5·0 than at pH 5·5. The minimum inhibitory concentrations (MICs) were comparable with the concentrations of these acids that have been reported in Danish Blue cheese, where they were probably formed by the metabolism of Penicillium roquefortii . Thus hexanoic and octanoic acids may contribute to the inhibition of listerias in some cheeses. 相似文献
997.
The effect of culture medium and aeration on growth of Listeria monocytogenes at pH 4.5 总被引:1,自引:1,他引:0
Listeria monocytogenes multiplied at 20°C in medium adjusted to pH 4.5 with HCl, and the lag before growth was eliminated when the inoculum was grown to log phase in the same medium. In a tryptone soya medium with yeast extract and added glucose, growth at pH 4.5 was more rapid than in a tryptose phosphate medium, and this difference was greater in air than under nitrogen. The results show that the bacterium was capable of more rapid growth in air than under nitrogen at this pH and suggest that the tryptose phosphate medium was nutritionally limiting for growth. 相似文献
998.
999.
1000.
1) Rat hepatocytes, stored in a simple salts medium for 24 h at 4°C, retain more than 80% of their capacity to synthesize glucose from lactate.
2) The combination of NH4Cl with oleate is cytotoxic during storage and during subsequent incubation of hepatocytes from 48 h starved rats, but not to hepatocytes from fed rats.
3) Protection against cytotoxicity is afforded by albumin and by a number of other compounds, notably polyols and glycerol.
4) These compounds appear to exert their effects by scavenging free radicals and, in the case of polyols and glycerol, by supplying reducing equivalents to maintain the redox state of the cell in the face of increased flux through glutathione peroxidase. 相似文献
2) The combination of NH4Cl with oleate is cytotoxic during storage and during subsequent incubation of hepatocytes from 48 h starved rats, but not to hepatocytes from fed rats.
3) Protection against cytotoxicity is afforded by albumin and by a number of other compounds, notably polyols and glycerol.
4) These compounds appear to exert their effects by scavenging free radicals and, in the case of polyols and glycerol, by supplying reducing equivalents to maintain the redox state of the cell in the face of increased flux through glutathione peroxidase. 相似文献