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71.
K Negishi T Yamashita W Zacharias R D Wells T Bessho H Hayatsu 《Nucleic acids symposium series》1990,(22):109-110
Salt-induced and supercoiling-induced B-Z junctions in pWR756, a plasmid containing (GC)16, were probed with bisulfite-methoxyamine, a modification reagent specific for single-stranded nucleic acids. The modification sites were analyzed with S1 nuclease and the modified cytosines were determined from termination sites of DNA chain elongation by DNA polymerase. The results showed that most accessible cytosines are the same for both types of B-Z junctions. 相似文献
72.
Molecular evidence and clinical importance of β‐arrestins expression in patients with acromegaly 下载免费PDF全文
Maria Caroline Alves Coelho Marina Lipkin Vasquez Luiz Eduardo Wildemberg Mari C. Vázquez‐Borrego Luciana Bitana Aline Helen da Silva Camacho Débora Silva Liana Lumi Ogino Nina Ventura Leila Chimelli Raul M. Luque Leandro Kasuki Mônica R. Gadelha 《Journal of cellular and molecular medicine》2018,22(4):2110-2116
β‐arrestins seem to have a role in endocytosis and desensitization of somatostatin receptor subtype 2 (sst2) and could be associated with the responsiveness to somatostatin receptor ligands (SRL) in patients with acromegaly. To investigate the in vivo correlation between β‐arrestins 1 and 2 with sst2, sst5 and dopamine receptor subtype 2 (D2) expressions, and the association of β‐arrestins with response to first‐generation SRL and invasiveness in somatotropinomas. β‐arrestins 1 and 2, sst2, sst5 and D2 mRNA expressions were evaluated by quantitative real‐time RT‐PCR on tumoral tissue of 96 patients. Moreover, sst2 and sst5 protein expressions were also evaluated in 40 somatotropinomas by immunohistochemistry. Response to SRL, defined as GH <1 μg/l and normal IGF‐I levels, was assessed in 40 patients. The Knosp‐Steiner criteria were used to define invasiveness. Median β‐arrestin 1, β‐arrestin 2, sst2, sst5 and D2 mRNA copy numbers were 478; 9375; 731; 156 ; and 3989, respectively. There was a positive correlation between β‐arrestins 1 and 2 (R = 0.444, P < 0.001). However, no correlation between β‐arrestins and sst2, sst5 (mRNA and protein levels) or D2 was found. No association was found between β‐arrestins expression and SRL responsiveness or tumour invasiveness. Although previous data suggest a putative correlation between β‐arrestins and sst2, our data clearly indicated that no association existed between β‐arrestins and sst2, sst5 or D2 expression, nor with response to SRL or tumour invasiveness. Therefore, further studies are required to clarify whether β‐arrestins have a role in the response to treatment with SRL in acromegaly. 相似文献
73.
This study examined a host-parasite relationship between chestnut goby Gymnogobius castaneus (O’Shaughnessy 1875) and a unionid mussel Anemina arcaeformis (Heude 1877) in three floodplain water bodies of the Ishikari River. Field observations of gravidity between summer (July 2016) and spring (May 2017) revealed that female A. arcaeformis began incubating eggs in September, and all reached a fully ripe-glochidia-gravid stage by May with a decreasing trend in the proportion of gravid individuals. Two laboratory experiments, in which field-caught G. castaneus were artificially infected by glochidia of A. arcaeformis, showed that transformation rates were among the highest ever reported for A. arcaeformis (an average across all the surviving individuals: 47.9%) with a shorter development time in warmer environments. Transformation rates did not differ among sympatric or allopatric pairs, nor decrease when infected twice. Relatively high transformation rates were attributed to high glochidia attachments to gills and mouth cavity (approximately 30% of initially attached individuals) following the highest to fins, or a reduced immunity against infection due to colder environments. Overall, our study demonstrated that chestnut goby (G. castaneus) served as a suitable host of winter-breeding A. arcaeformis. Together with records of the expanded distribution of these two species in the past 30 years, it might be relatively recently that these two have colonized the study areas after extensive land changes, with A. arcaeformis having benefited from a successful host-parasite relationship. 相似文献
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To determine a possible mechanism causing male and female sterility in Cryptomeria japonica male and female cones were collected from a C. japonica, tree, ShinDai2, that lacks pollen release and fertile seeds and specimens were processed to examine the development of pollen
and female gametophytes using light microscopy and field emission scanning electron microscopy. Pre-meiotic development proceeded
normally, but the formation of aberrant meiotic products was observed in cones of both sexes. In sterile microsporangia, heterogeneous
microspore populations ranging from monads to polyads gave rise to mature pollen grains of non-uniform size. These pollen
grains were covered with an amorphous layer and adhered to each other. In addition, they remained in the microsporangia and
were not released even after the onset of pollen dissemination from fertile trees. In the ovules of sterile female cones,
megaspores with abnormal shapes, numbers, and sizes formed, and the development of female gametophytes was arrested at the
free nuclear or archegonium formation stages. These gametophytes collapsed, and no fertile embryo was generated. Results indicate
that meiotic defects are important in the sterility mechanism. 相似文献
79.
Prolyl 4-Hydroxylation of ��-Fibrinogen: A NOVEL PROTEIN MODIFICATION REVEALED BY PLASMA PROTEOMICS*
Masaya Ono Junichi Matsubara Kazufumi Honda Tomohiro Sakuma Tomoyo Hashiguchi Hiroshi Nose Shoji Nakamori Takuji Okusaka Tomoo Kosuge Naohiro Sata Hideo Nagai Tatsuya Ioka Sachiko Tanaka Akihiko Tsuchida Tatsuya Aoki Masashi Shimahara Yohichi Yasunami Takao Itoi Fuminori Moriyasu Ayako Negishi Hideya Kuwabara Ayako Shoji Setsuo Hirohashi Tesshi Yamada 《The Journal of biological chemistry》2009,284(42):29041-29049
Plasma proteome analysis requires sufficient power to compare numerous samples and detect changes in protein modification, because the protein content of human samples varies significantly among individuals, and many plasma proteins undergo changes in the bloodstream. A label-free proteomics platform developed in our laboratory, termed “Two-Dimensional Image Converted Analysis of Liquid chromatography and mass spectrometry (2DICAL),” is capable of these tasks. Here, we describe successful detection of novel prolyl hydroxylation of α-fibrinogen using 2DICAL, based on comparison of plasma samples of 38 pancreatic cancer patients and 39 healthy subjects. Using a newly generated monoclonal antibody 11A5, we confirmed the increase in prolyl-hydroxylated α-fibrinogen plasma levels and identified prolyl 4-hydroxylase A1 as a key enzyme for the modification. Competitive enzyme-linked immunosorbent assay of 685 blood samples revealed dynamic changes in prolyl-hydroxylated α-fibrinogen plasma level depending on clinical status. Prolyl-hydroxylated α-fibrinogen is presumably controlled by multiple biological mechanisms, which remain to be clarified in future studies.For comprehensive analysis of plasma proteins, it is necessary to compare a sufficient number of blood samples to avoid simple interindividual heterogeneity, because the protein content of human samples varies significantly among individuals. Also, the provision of sufficient power is needed to detect protein modification because many plasma proteins undergo changes in the bloodstream (1). Even though the proteomic technologies have advanced (2, 3), there remains room for improvement. Different isotope labeling and identification-based methods have been developed for quantitative proteomics technologies (4–6), but the number of samples that can be compared by the current isotope-labeling methods is limited, and identification-based proteomics is unable to capture information regarding unknown modifications.A label-free proteomics platform developed in our laboratory, termed “Two-Dimensional Image Converted Analysis of Liquid chromatography and mass spectrometry (2DICAL)2 (7), simply compares the liquid chromatography and mass spectrometry (LC-MS) data and detects a protein modification by finding changes in the mass to charge ratio (m/z) and retention time (RT). Enhanced methods for accurate MS peak alignment across multiple LC runs have enabled the successful implementation of clinical studies requiring comparison of a large number of samples (8, 9). Using 2DICAL to analyze plasma samples of pancreatic cancer patients and healthy controls, novel prolyl hydroxylation of α-fibrinogen was successfully discovered.Fibrinogen and its modification has been investigated because of its clinical importance (10, 11). On the other hand, prolyl hydroxylation has attracted attention after the discovery of the hypoxia-inducible factor 1α (HIF1α) prolyl-hydroxylase and its role in switching of HIF1α functions (12). Prolyl hydroxylation in other proteins has been energetically sought, but only a few such proteins have been identified (13). Only one study has reported prolyl hydroxylation of fibrinogen at the β chain (14).Here, we report the detection of prolyl 4-hydroxylated α-fibrinogen by plasma proteome analysis, a protein modification that dynamically changes in plasma depending on the clinical status and is a candidate plasma biomarker. 相似文献
80.
Nobuyuki Kimura Makoto Inoue Sachi Okabayashi Fumiko Ono Takayuki Negishi 《The Journal of biological chemistry》2009,284(45):31291-31302
Growing evidence suggests that endocytic dysfunction is intimately involved in early stage Alzheimer disease pathology, such as the accumulation of β-amyloid precursor protein in enlarged early endosomes. However, it remains unclear how endocytic dysfunction is induced in an age-dependent manner. Cytoplasmic dynein, a microtubule-based motor protein, interacts with another microtubule-associated protein, dynactin. The resulting dynein-dynactin complex mediates minus end-directed vesicle transport, including endosome trafficking. We have previously shown that the interaction between dynein-dynactin complexes is clearly attenuated in aged monkey brains, suggesting that dynein-mediated transport dysfunction exists in aged brains. Our immunohistochemical analyses revealed that age-dependent endocytic pathology was accompanied by an increase in Rab GTPases in aged monkey brains. Here, we demonstrated that siRNA-induced dynein dysfunction reproduced the endocytic pathology accompanied by increased Rab GTPases seen in aged monkey brains and significantly disrupted exosome release. Moreover, it also resulted in endosomal β-amyloid precursor protein accumulation characterized by increased β-site cleavage. These findings suggest that dynein dysfunction may underlie age-dependent endocytic dysfunction via the up-regulation of Rab GTPases. In addition, this vicious circle may worsen endocytic dysfunction, ultimately leading to Alzheimer disease pathology. 相似文献