全文获取类型
收费全文 | 2354篇 |
免费 | 221篇 |
国内免费 | 2篇 |
专业分类
2577篇 |
出版年
2024年 | 8篇 |
2023年 | 30篇 |
2022年 | 51篇 |
2021年 | 119篇 |
2020年 | 65篇 |
2019年 | 76篇 |
2018年 | 80篇 |
2017年 | 60篇 |
2016年 | 111篇 |
2015年 | 153篇 |
2014年 | 153篇 |
2013年 | 174篇 |
2012年 | 203篇 |
2011年 | 175篇 |
2010年 | 116篇 |
2009年 | 106篇 |
2008年 | 130篇 |
2007年 | 118篇 |
2006年 | 102篇 |
2005年 | 115篇 |
2004年 | 78篇 |
2003年 | 55篇 |
2002年 | 53篇 |
2001年 | 23篇 |
2000年 | 10篇 |
1999年 | 13篇 |
1998年 | 18篇 |
1997年 | 7篇 |
1996年 | 9篇 |
1995年 | 5篇 |
1994年 | 8篇 |
1993年 | 6篇 |
1992年 | 9篇 |
1991年 | 7篇 |
1990年 | 7篇 |
1989年 | 9篇 |
1988年 | 12篇 |
1987年 | 5篇 |
1986年 | 7篇 |
1985年 | 4篇 |
1982年 | 3篇 |
1981年 | 3篇 |
1980年 | 7篇 |
1979年 | 3篇 |
1978年 | 4篇 |
1971年 | 5篇 |
1968年 | 4篇 |
1963年 | 4篇 |
1950年 | 3篇 |
1919年 | 2篇 |
排序方式: 共有2577条查询结果,搜索用时 0 毫秒
991.
992.
Year‐round movements of sympatric Fork‐tailed (Oceanodroma furcata) and Leach's (O. leucorhoa) storm‐petrels 下载免费PDF全文
Luke R. Halpin Ingrid L. Pollet Christopher Lee Ken H. Morgan Harry R. Carter 《Journal of Field Ornithology》2018,89(3):207-220
Long‐distance movements are characteristic of most seabirds in the order Procellariiformes. However, little is known about the migration and foraging ranges of many of the smaller species in this order, especially storm‐petrels (Hydrobatidae). We used Global Location Sensors to document the year‐round movements of sympatrically breeding Fork‐tailed Storm‐Petrels (Oceanodroma furcata) and Leach's Storm‐Petrels (O. leucorhoa) from the Gillam Islands located northwest of Vancouver Island, British Columbia, Canada. In 2016, breeding Fork‐tailed (N = 5) and Leach's (N = 2) storm‐petrels traveled maximum distances of ~1550–1600 km from their colony to a region that has a wide shelf with major canyons creating a highly productive foraging area. After the breeding season, Fork‐tailed Storm‐Petrels (N = 2) traveled to similar areas west of the Gillam Islands, a maximum distance of ~3600 km from the breeding colony, and remained in the North Pacific Ocean and north of the Subarctic Boundary for an average of 5.4 mo. Post‐breeding Leach's Storm‐Petrels (N = 2) moved south to the Eastern Tropical Pacific, west of central Mexico, Ecuador, and northern Peru, an estimated maximum distance of ~6700 km from their breeding colony, and remained there for an average of 7.2 mo. Carbon (δ13C) and nitrogen (δ15N) stable isotope analyses of feathers revealed niche separation between Fork‐tailed (N = 21) and Leach's (N = 53) storm‐petrels. The wide range of δ15N values in the feathers of Leach's Storm‐Petrels (N = 53) suggests that they foraged at a variety of trophic levels during the non‐breeding season. Our results demonstrate that storm‐petrels have large core foraging areas and occupy vast oceanic areas in the Pacific during their annual cycle. However, given the coarse precision of Global Location Sensors, additional study is needed to identify the specific areas used by each species during both breeding and non‐breeding periods. 相似文献
993.
William D. Pearse A. Márcia Barbosa Susanne A. Fritz Sally A. Keith Luke J. Harmon John Harte Daniele Silvestro Xiao Xiao T. Jonathan Davies 《Journal of Biogeography》2018,45(6):1223-1230
Linking pattern to process across spatial and temporal scales has been a key goal of the field of biogeography. In January 2017, the 8th biennial conference of the International Biogeography Society sponsored a symposium on Building up biogeography—process to pattern that aimed to review progress towards this goal. Here we present a summary of the symposium, in which we identified promising areas of current research and suggested future research directions. We focus on (1) emerging types of data such as behavioural observations and ancient DNA, (2) how to better incorporate historical data (such as fossils) to move beyond what we term “footprint measures” of past dynamics and (3) the role that novel modelling approaches (e.g. maximum entropy theory of ecology and approximate Bayesian computation) and conceptual frameworks can play in the unification of disciplines. We suggest that the gaps separating pattern and process are shrinking, and that we can better bridge these aspects by considering the dimensions of space and time simultaneously. 相似文献
994.
DNA Barcoding is a powerful molecular biology tool for the identification of species, analysis of gene flow from within and between populations and evaluating species concepts. The process can be incorporated into a college genetics or bioinformatics core curriculum. Here we demonstrate how, by simply using Plecoptera, we actively engaged students in DNA extraction, amplification and DNA Barcoding. As a result, 38 new DNA sequences were accepted by an international DNA database, with potentially three of them being new species to the database. More importantly, through multiple assessment measurements, students showed a high degree of learning took place and that all student learning objectives were met. 相似文献
995.
Luke J. Mortensen Oren Levy Joseph P. Phillips Tara Stratton Brian Triana Juan P. Ruiz Fangqi Gu Jeffrey M. Karp Charles P. Lin 《PloS one》2013,8(10)
The ability to deliver cells to appropriate target tissues is a prerequisite for successful cell-based therapy. To optimize cell therapy it is therefore necessary to develop a robust method of in vivo cell delivery quantification. Here we examine Mesenchymal Stem Cells (MSCs) labeled with a series of 4 membrane dyes from which we select the optimal dye combination for pair-wise comparisons of delivery to inflamed tissue in the mouse ear using confocal fluorescence imaging. The use of an optimized dye pair for simultaneous tracking of two cell populations in the same animal enables quantification of a test population that is referenced to an internal control population, thereby eliminating intra-subject variations and variations in injected cell numbers. Consistent results were obtained even when the administered cell number varied by more than an order of magnitude, demonstrating an ability to neutralize one of the largest sources of in vivo experimental error and to greatly reduce the number of cells required to evaluate cell delivery. With this method, we are able to show a small but significant increase in the delivery of cytokine pre-treated MSCs (TNF-α & IFN-γ) compared to control MSCs. Our results suggest future directions for screening cell strategies using our in vivo cell delivery assay, which may be useful to develop methods to maximize cell therapeutic potential. 相似文献
996.
Laura E. McCoy Lucy Rutten Dan Frampton Ian Anderson Luke Granger Rachael Bashford-Rogers Gillian Dekkers Nika M. Strokappe Michael S. Seaman Willie Koh Vanina Grippo Alexander Kliche Theo Verrips Paul Kellam Ariberto Fassati Robin A. Weiss 《PLoS pathogens》2014,10(12)
To date, no immunization of humans or animals has elicited broadly neutralizing sera able to prevent HIV-1 transmission; however, elicitation of broad and potent heavy chain only antibodies (HCAb) has previously been reported in llamas. In this study, the anti-HIV immune responses in immunized llamas were studied via deep sequencing analysis using broadly neutralizing monoclonal HCAbs as a guides. Distinct neutralizing antibody lineages were identified in each animal, including two defined by novel antibodies (as variable regions called VHH) identified by robotic screening of over 6000 clones. The combined application of five VHH against viruses from clades A, B, C and CRF_AG resulted in neutralization as potent as any of the VHH individually and a predicted 100% coverage with a median IC50 of 0.17 µg/ml for the panel of 60 viruses tested. Molecular analysis of the VHH repertoires of two sets of immunized animals showed that each neutralizing lineage was only observed following immunization, demonstrating that they were elicited de novo. Our results show that immunization can induce potent and broadly neutralizing antibodies in llamas with features similar to human antibodies and provide a framework to analyze the effectiveness of immunization protocols. 相似文献
997.
Luke T. Krebs Christa Starling Alexander V. Chervonsky Thomas Gridley 《Genesis (New York, N.Y. : 2000)》2010,48(3):146-150
Notch signaling is essential for embryonic vascular development in mammals and other vertebrates. Here we show that mouse embryos with conditional activation of the Notch1 gene in endothelial cells (Notch1 gain of function embryos) exhibit defects in vascular remodeling increased diameter of the dorsal aortae, and form arteriovenous malformations. Conversely, embryos with either constitutive or endothelial cell‐specific Notch1 gene deletion also have vascular defects, but exhibit decreased diameter of the dorsal aortae and form arteriovenous malformations distinctly different from the Notch1 gain of function mutants. Surprisingly, embryos homozygous for mutations of the ephrinB/EphB pathway genes Efnb2 and Ephb4 exhibit vascular defects and arteriovenous malformations that phenocopy the Notch1 gain of function mutants. These results suggest that formation of arteriovenous malformations in Notch1 gain of function mutants and ephrinB/EphB pathway loss of function mutant embryos occurs by different mechanisms. genesis 48:146–150, 2010. © 2010 Wiley‐Liss, Inc. 相似文献
998.
999.
Indices of relative abundance do not control for variation in detectability, which can bias density estimates such that ecological processes are difficult to infer. Distance sampling methods can be used to correct for detectability, but in rainforest, where dense vegetation and diverse assemblages complicate sampling, information is lacking about factors affecting their application. Rare species present an additional challenge, as data may be too sparse to fit detection functions. We present analyses of distance sampling data collected for a diverse tropical rainforest bird assemblage across broad elevational and latitudinal gradients in North Queensland, Australia. Using audio and visual detections, we assessed the influence of various factors on Effective Strip Width (ESW), an intuitively useful parameter, since it can be used to calculate an estimate of density from count data. Body size and species exerted the most important influence on ESW, with larger species detectable over greater distances than smaller species. Secondarily, wet weather and high shrub density decreased ESW for most species. ESW for several species also differed between summer and winter, possibly due to seasonal differences in calling behavior. Distance sampling proved logistically intensive in these environments, but large differences in ESW between species confirmed the need to correct for detection probability to obtain accurate density estimates. Our results suggest an evidence-based approach to controlling for factors influencing detectability, and avenues for further work including modeling detectability as a function of species characteristics such as body size and call characteristics. Such models may be useful in developing a calibration for non-distance sampling data and for estimating detectability of rare species. 相似文献
1000.
Studies of social networks, mapped using self-reported contacts, have demonstrated the strong influence of social connections on the propensity for individuals to adopt or maintain healthy behaviors and on their likelihood to adopt health risks such as obesity. Social network analysis may prove useful for businesses and organizations that wish to improve the health of their populations by identifying key network positions. Health traits have been shown to correlate across friendship ties, but evaluating network effects in large coworker populations presents the challenge of obtaining sufficiently comprehensive network data. The purpose of this study was to evaluate methods for using online communication data to generate comprehensive network maps that reproduce the health-associated properties of an offline social network. In this study, we examined three techniques for inferring social relationships from email traffic data in an employee population using thresholds based on: (1) the absolute number of emails exchanged, (2) logistic regression probability of an offline relationship, and (3) the highest ranked email exchange partners. As a model of the offline social network in the same population, a network map was created using social ties reported in a survey instrument. The email networks were evaluated based on the proportion of survey ties captured, comparisons of common network metrics, and autocorrelation of body mass index (BMI) across social ties. Results demonstrated that logistic regression predicted the greatest proportion of offline social ties, thresholding on number of emails exchanged produced the best match to offline network metrics, and ranked email partners demonstrated the strongest autocorrelation of BMI. Since each method had unique strengths, researchers should choose a method based on the aspects of offline behavior of interest. Ranked email partners may be particularly useful for purposes related to health traits in a social network. 相似文献