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21.
Irina V. Elanskaya Dmitry V. Zlenko Evgeny P. Lukashev Natalia E. Suzina Irena A. Kononova Igor N. Stadnichuk 《BBA》2018,1859(4):280-291
Phycobilisome (PBS) is a giant photosynthetic antenna associated with the thylakoid membranes of cyanobacteria and red algae. PBS consists of two domains: central core and peripheral rods assembled of disc-shaped phycobiliprotein aggregates and linker polypeptides. The study of the PBS architecture is hindered due to the lack of the data on the structure of the large ApcE-linker also called LCM. ApcE participates in the PBS core stabilization, PBS anchoring to the photosynthetic membrane, transfer of the light energy to chlorophyll, and, very probably, the interaction with the orange carotenoid protein (OCP) during the non-photochemical PBS quenching. We have constructed the cyanobacterium Synechocystis sp. PCC 6803 mutant lacking 235 N-terminal amino acids of the chromophorylated PBLCM domain of ApcE. The altered fluorescence characteristics of the mutant PBSs indicate that the energy transfer to the terminal emitters within the mutant PBS is largely disturbed. The PBSs of the mutant become unable to attach to the thylakoid membrane, which correlates with the identified absence of the energy transfer from the PBSs to the photosystem II. At the same time, the energy transfer from the PBS to the photosystem I was registered in the mutant cells and seems to occur due to the small cylindrical CpcG2-PBSs formation in addition to the conventional PBSs. In contrast to the wild type Synechocystis, the OCP-mediated non-photochemical PBS quenching was not registered in the mutant cells. Thus, the PBLCM domain takes part in formation of the OCP binding site in the PBS. 相似文献
22.
The conduction of protons in different stereoisomers of dioxolane-linked gramicidin A channels
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Two different stereoisomers of the dioxolane-linked gramicidin A (gA) channels were individually synthesized (the SS and RR dimers;. Science. 244:813-817). The structural differences between these dimers arise from different chiralities within the dioxolane linker. The SS dimer mimics the helicity and the inter- and intramolecular hydrogen bonding of the monomer-monomer association of gA's. In contrast, there is a significant disruption of the helicity and hydrogen bonding pattern of the ion channel in the RR dimer. Single ion channels formed by the SS and RR dimers in planar lipid bilayers have different proton transport properties. The lipid environment in which the different dimers are reconstituted also has significant effects on single-channel proton conductance (g(H)). g(H) in the SS dimer is about 2-4 times as large as in the RR. In phospholipid bilayers with 1 M [H(+)](bulk), the current-voltage (I-V) relationship of the SS dimer is sublinear. Under identical experimental conditions, the I-V plot of the RR dimer is supralinear (S-shaped). In glycerylmonooleate bilayers with 1 M [H(+)](bulk), both the SS and RR dimers have a supralinear I-V plot. Consistent with results previously published (. Biophys. J. 73:2489-2502), the SS dimer is stable in lipid bilayers and has fast closures. In contrast, the open state of the RR channel has closed states that can last a few seconds, and the channel eventually inactivates into a closed state in either phospholipid or glycerylmonooleate bilayers. It is concluded that the water dynamics inside the pore as related to proton wire transfer is significantly different in the RR and SS dimers. Different physical mechanisms that could account for this hypothesis are discussed. The gating of the synthetic gA dimers seems to depend on the conformation of the dioxolane link between gA's. The experimental results provide an important framework for a detailed investigation at the atomic level of proton conduction in different and relatively simple ion channel structures. 相似文献
23.
Igor N. Stadnichuk Evgeny P. Lukashev Irina V. Elanskaya 《Photosynthesis research》2009,99(3):227-241
The features of the two types of short-term light-adaptations of photosynthetic apparatus, State 1/State 2 transitions, and
non-photochemical fluorescence quenching of phycobilisomes (PBS) by orange carotene-protein (OCP) were compared in the cyanobacterium
Synechocystis sp. PCC 6803 wild type, CK pigment mutant lacking phycocyanin, and PAL mutant totally devoid of phycobiliproteins. The permanent
presence of PBS-specific peaks in the in situ action spectra of photosystem I (PSI) and photosystem II (PSII), as well as
in the 77 K fluorescence excitation spectra for chlorophyll emission at 690 nm (PSII) and 725 nm (PSI) showed that PBS are
constitutive antenna complexes of both photosystems. The mutant strains compensated the lack of phycobiliproteins by higher
PSII content and by intensification of photosynthetic linear electron transfer. The detectable changes of energy migration
from PBS to the PSI and PSII in the Synechocystis wild type and the CK mutant in State 1 and State 2 according to the fluorescence excitation spectra measurements were not
registered. The constant level of fluorescence emission of PSI during State 1/State 2 transitions and simultaneous increase
of chlorophyll fluorescence emission of PSII in State 1 in Synechocystis PAL mutant allowed to propose that spillover is an unlikely mechanism of state transitions. Blue–green light absorbed by
OCP diminished the rout of energy from PBS to PSI while energy migration from PBS to PSII was less influenced. Therefore,
the main role of OCP-induced quenching of PBS is the limitation of PSI activity and cyclic electron transport under relatively
high light conditions. 相似文献
24.
I. N. Stadnichuk M. F. Yanyushin V. A. Boychenko E. P. Lukashev E. N. Boldareva A. A. Solovyev V. M. Gorlenko 《Microbiology》2009,78(1):7-15
Bioenergetics of the aerobic bacteriochlorophyll a-containing (BCl a) bacterium (ABC bacterium) Roseinatronobacter thiooxidans is a combination of photosynthesis, oxygen respiration, and oxidation of sulfur compounds under alkaliphilic conditions. The photosynthetic activity of Rna. thiooxidans cells was established by the photoinhibition of cell respiration and reversible photobleaching discoloration of the BCl a of reaction centers (RC), connected by the chain of electron transfer with cytochrome c 551 oxidation. The species under study, like many purple bacteria and some of the known ABC bacteria, possesses a light-harvesting pigment-protein (LHI) complex with the average number of 30 molecules of antenna BCl a per one photosynthetic RC. Under microaerobic growth conditions, the cells contained bc 1 complex and two terminal oxidases: cbb 3-cytochrome oxidase and the alternative cytochrome oxidase of the a 3 type. Besides, Rna. thiooxidans was shown to have several different soluble low- and high-potential cytochromes c, probably associated with the ability of utilizing sulfur compounds as additional electron donors. 相似文献
25.
P. P. Knox E. P. Lukashev N. Kh. Seifullina V. V. Gorokhov A. B. Rubin 《Biophysics》2017,62(5):734-741
The mechanisms of the specific influence of dipyridamole therapy remain as a subject of research. In the present work the effects of dipyridamole and some its derivatives on the energization state of photosynthetic membranes of purple bacteria chromatophores have been studied. It was shown that under illumination of chromatophores in the presence of dipyridamole the occurrence of its radical form was observed due to the presence of electron-proton donation properties. These properties are vital to trigger the energization of a functionally active photosynthetic membrane when it is illuminated in the presence of dipyridamole and its derivatives. It is obvious that these properties of dipyridamole should be taken into account while studying the effects of this agent as a medical preparation with specific action. 相似文献
26.
E. P. Lukashev P. P. Knox A. B. Rubin M. V. Olenchuk Yu. M. Barabash N. M. Berezetskaya V. N. Kharkyanen 《Biophysics》2009,54(3):296-301
We studied the kinetics of dark recombination of charges photoseparated between the bacteriochlorophyll dimer (P) and the quinone acceptors of the photosynthetic reaction centers (RCs) of Rhodobacter sphaeroides. The time of light activation was varied from 1 to 60 s. The relaxation rate constant distribution was computed from experimental kinetic curves of charge recombination, using our original method whereby relaxation curves are approximated by a set of Gaussian-like peaks in the time domain that correspond to different conformational sub-states of RCs. With increasing photoactivation exposure, some peaks shift toward longer relaxation times. A ‘bifurcation’ of the slower peak is observed at exposures of 20–30 s. The phenomenon is interpreted as evidence of a conformational transition induced by separated charges in the RC structure. 相似文献
27.
E A Lukashev 《Biofizika》1991,36(1):78-82
The phenomenological model of T. Teorell's "biomembrane generator" underlies the mathematical model of erythrocyte forms as an autowave process. For a distributed system the model is formulated as a differential equation of the second order in partial derivatives with a small parameter. It is stated that a substantial thing in the model is the presence of mass-transfer through a pore membrane determined by its electrostatic and hydrostatic permeabilities, as well as the fact that mass-transfer through the membrane results in a non-uniform distribution of the liquid current through it. It is suggested that as a consequence of this process there appears mechanical instability of the spherical membrane, which is the cause of the autowave process. Due to the membrane elasticity this process can be compared with the movement of "tank caterpillar". 相似文献
28.
29.
Krasilnikov P. M. Lukashev E. P. Knox P. P. Seyfullina N. Kh. Rubin A. B. 《Biophysics》2018,63(6):895-905
Biophysics - The temperature dependence of the efficiency of energy transfer from polymer coated CdSe/CdS/ZnS quantum dots bearing terminal carboxyl groups to the reaction centers of purple... 相似文献
30.