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971.
Richardson A Wojciechowski T Franke R Schreiber L Kerstiens G Jarvis M Fricke W 《Planta》2007,225(6):1471-1481
The developing leaf three of barley provides an excellent model system for the direct determination of relationships between
amounts of waxes and cutin and cuticular permeance. Permeance of the cuticle was assessed via the time-course of uptake of
either toluidine blue or 14C-labelled benzoic acid ([14C] BA) along the length of the developing leaf. Toluidine blue uptake only occurred within the region 0–25 mm from the point
of leaf insertion (POLI). Resistance—the inverse of permeance—to uptake of [14C] BA was determined for four leaf regions and was lowest in the region 10–20 mm above POLI. At 20–30 and 50–60 mm above POLI,
it increased by factors of 6 and a further 32, respectively. Above the point of emergence of leaf three from the sheath of
leaf two, which was 76–80 mm above POLI, resistance was as high as at 50–60 mm above POLI. GC-FID/MS analyses of wax and cutin
showed that: (1) the initial seven fold increase in cuticular resistance coincided with increase in cutin coverage and appearance
of waxes; (2) the second, larger and final increase in cuticle resistance was accompanied by an increase in wax coverage,
whereas cutin coverage remained unchanged; (3) cutin deposition in barley leaf epidermis occurred in parallel with cell elongation,
whereas deposition of significant amounts of wax commenced as cells ceased to elongate. 相似文献
972.
Wijaya Lukas S. Rau Carina Braun Theresa S. Marangoz Serif Spegg Vincent Vlasveld Matthijs Albrecht Wiebke Brecklinghaus Tim Kamp Hennicke Beltman Joost B. Hengstler Jan G. van de Water Bob Leist Marcel Schildknecht Stefan 《Cell biology and toxicology》2022,38(5):847-864
Cell Biology and Toxicology - Toxicity is not only a function of damage mechanisms, but is also determined by cellular resilience factors. Glutathione has been reported as essential element to... 相似文献
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Teodor E. Yordanov Victoria E. B. Hipolito Gudrun Liebscher Georg F. Vogel Taras Stasyk Caroline Herrmann Stephan Geley David Teis Roberto J. Botelho Michael W. Hess Lukas A. Huber 《Traffic (Copenhagen, Denmark)》2019,20(9):674-696
Mechanisms that control lysosomal function are essential for cellular homeostasis. Lysosomes adapt in size and number to cellular needs but little is known about the underlying molecular mechanism. We demonstrate that the late endosomal/lysosomal multimeric BLOC‐1‐related complex (BORC) regulates the size of these organelles via PIKfyve‐dependent phosphatidylinositol‐3,5‐bisphosphate [PI(3,5)P2] production. Deletion of the core BORC component Diaskedin led to increased levels of PI(3,5)P2, suggesting activation of PIKfyve, and resulted in enhanced lysosomal reformation and subsequent reduction in lysosomal size. This process required AMP‐activated protein kinase (AMPK), a known PIKfyve activator, and was additionally dependent on the late endosomal/lysosomal adaptor, mitogen‐activated protein kinases and mechanistic target of rapamycin activator (LAMTOR/Ragulator) complex. Consistently, in response to glucose limitation, AMPK activated PIKfyve, which induced lysosomal reformation with increased baseline autophagy and was coupled to a decrease in lysosomal size. These adaptations of the late endosomal/lysosomal system reversed under glucose replete growth conditions. In summary, our results demonstrate that BORC regulates lysosomal reformation and size in response to glucose availability. 相似文献
976.
Tobias Abzieher Somayeh Moghadamzadeh Fabian Schackmar Helge Eggers Florian Sutterlüti Amjad Farooq Danny Kojda Klaus Habicht Raphael Schmager Adrian Mertens Raheleh Azmi Lukas Klohr Jonas A. Schwenzer Michael Hetterich Uli Lemmer Bryce S. Richards Michael Powalla Ulrich W. Paetzold 《Liver Transplantation》2019,9(12)
High‐quality charge carrier transport materials are of key importance for stable and efficient perovskite‐based photovoltaics. This work reports on electron‐beam‐evaporated nickel oxide (NiOx) layers, resulting in stable power conversion efficiencies (PCEs) of up to 18.5% when integrated into solar cells employing inkjet‐printed perovskite absorbers. By adding oxygen as a process gas and optimizing the layer thickness, transparent and efficient NiOx hole transport layers (HTLs) are fabricated, exhibiting an average absorptance of only 1%. The versatility of the material is demonstrated for different absorber compositions and deposition techniques. As another highlight of this work, all‐evaporated perovskite solar cells employing an inorganic NiOx HTL are presented, achieving stable PCEs of up to 15.4%. Along with good PCEs, devices with electron‐beam‐evaporated NiOx show improved stability under realistic operating conditions with negligible degradation after 40 h of maximum power point tracking at 75 °C. Additionally, a strong improvement in device stability under ultraviolet radiation is found if compared to conventional perovskite solar cell architectures employing other metal oxide charge transport layers (e.g., titanium dioxide). Finally, an all‐evaporated perovskite solar mini‐module with a NiOx HTL is presented, reaching a PCE of 12.4% on an active device area of 2.3 cm2. 相似文献
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Gowri?Nayak Lukas?Varga Claire?Trincot Mohsin?Shahzad Penelope?L.?Friedman Iwar?Klimes John?H.?GreinwaldJr. S.?Amer?Riazuddin Ivica?Masindova Milan?Profant Shaheen?N.?Khan Thomas?B.?Friedman Zubair?M.?Ahmed Daniela?Gasperikova Sheikh?Riazuddin Saima?RiazuddinEmail author 《Human genetics》2015,134(4):423-437
Pathogenic mutations of MARVELD2, encoding tricellulin, a tricelluar tight junction protein, cause autosomal recessive non-syndromic hearing loss (DFNB49) in families of Pakistan and Czech Roma origin. In fact, they are a significant cause of prelingual hearing loss in the Czech Roma, second only to GJB2 variants. Previously, we reported that mice homozygous for p.Arg497* variant of Marveld2 had a broad phenotypic spectrum, where defects were observed in the inner ear, heart, mandibular salivary gland, thyroid gland and olfactory epithelium. The current study describes the types and frequencies of MARVELD2 alleles and clinically reexamines members of DFNB49 families. We found that MARVELD2 variants are responsible for about 1.5 % (95 % CI 0.8–2.6) of non-syndromic hearing loss in our cohort of 800 Pakistani families. The c.1331+2T>C allele is recurrent. In addition, we identified a novel large deletion in a single family, which appears to have resulted from non-allelic homologous recombination between two similar Alu short interspersed elements. Finally, we observed no other clinical manifestations co-segregating with hearing loss in DFNB49 human families, and hypothesize that the additional abnormalities in the Marveld2 mutant mouse indicates a critical non-redundant function for tricellulin in other organ systems. 相似文献