全文获取类型
收费全文 | 5970篇 |
免费 | 354篇 |
出版年
2024年 | 8篇 |
2023年 | 44篇 |
2022年 | 97篇 |
2021年 | 183篇 |
2020年 | 129篇 |
2019年 | 172篇 |
2018年 | 209篇 |
2017年 | 201篇 |
2016年 | 267篇 |
2015年 | 385篇 |
2014年 | 387篇 |
2013年 | 443篇 |
2012年 | 513篇 |
2011年 | 487篇 |
2010年 | 304篇 |
2009年 | 230篇 |
2008年 | 368篇 |
2007年 | 345篇 |
2006年 | 310篇 |
2005年 | 253篇 |
2004年 | 254篇 |
2003年 | 194篇 |
2002年 | 164篇 |
2001年 | 37篇 |
2000年 | 30篇 |
1999年 | 35篇 |
1998年 | 46篇 |
1997年 | 19篇 |
1996年 | 27篇 |
1995年 | 17篇 |
1994年 | 14篇 |
1993年 | 10篇 |
1992年 | 18篇 |
1991年 | 9篇 |
1990年 | 4篇 |
1989年 | 12篇 |
1988年 | 6篇 |
1986年 | 9篇 |
1985年 | 7篇 |
1984年 | 12篇 |
1983年 | 6篇 |
1982年 | 9篇 |
1981年 | 7篇 |
1980年 | 5篇 |
1979年 | 5篇 |
1978年 | 9篇 |
1977年 | 4篇 |
1974年 | 2篇 |
1972年 | 2篇 |
1961年 | 3篇 |
排序方式: 共有6324条查询结果,搜索用时 15 毫秒
981.
Regis de A. Farani Wagner M. Teles Carlos B. Pinheiro Kasslio J. Guedes Klaus Krambrock Maria Irene Yoshida Luiz Fernando C. de Oliveira Flvia C. Machado 《Inorganica chimica acta》2008,361(7):2045-2050
The synthesis and structural characterization of the 2-D Cu(II) coordination polymer namely {[Cu2(BPP)4(NCS)4]}n, where BPP is the nitrogen ligand 1,3-bis(4-pyridyl)propane, are described. Single crystal diffraction analysis shows that the asymmetric unit consists on two crystallographically independent Cu(II) ions that adopt a distorted octahedral geometry. Each Cu(II) center is coordinated by four nitrogen atoms from different BPP ligands and by other two nitrogen atoms from isothiocyanate groups. The BPP ligands link the metal centers generating an undulated two-dimensional net of (4, 4) topology. Two sets of two-dimensional sheets interlock each other in the same plane, giving rise to a twofold parallel interpenetrating network. EPR spectra indicate no magnetic coupling of the two individual Cu2+ centers. 相似文献
982.
Chorismate mutase (CM) catalyzes the rearrangement of chorismate to prephenate in the biosynthetic pathway that forms phenylalanine and tyrosine in bacteria, fungi, plants, and apicomplexan parasites. Since this enzyme is absent from mammals, it represents a promising target for the development of new antimycobacterial drugs, which are needed to combat Mycobacterium tuberculosis, the causative agent of tuberculosis. Until recently, two putative open reading frames (ORFs), Rv0948c and Rv1885c, showing low sequence similarity to CMs have been described as "conserved hypothetical proteins" in the M. tuberculosis genome. However, we and others demonstrated that these ORFs are in fact monofunctional CMs of the AroQ structural class and that they are differentially localized in the mycobacterial cell. Since homologues to the M. tuberculosis enzymes are also present in Mycobacterium smegmatis, we cloned the coding sequences corresponding to ORFs MSMEG5513 and MSMEG2114 from the latter. The CM activities of both ORFs was determined, as well as their translational start sites. In addition, we analyzed the promoter activities of three M. tuberculosis loci related to phenylalanine and tyrosine biosynthesis under a variety of conditions using M. smegmatis as a surrogate host. Our results indicate that the aroQ (Rv0948c), *aroQ (Rv1885c), and fbpB (Rv1886c) genes from M. tuberculosis are constitutively expressed or subjected to minor regulation by aromatic amino acids levels, especially tryptophan. 相似文献
983.
Alfredo A. Carlini Alfredo E. Zurita Gustavo J. Scillato-Yané Rodolfo Sánchez Orangel A. Aguilera 《Pal?ontologische Zeitschrift》2008,82(2):139-152
One of the basal Glyptodontidae groups is represented by the Propalaehoplophorinae (late Oligocene — middle Miocene), whose genera (Propalaehoplophorus, Eucinepeltus, Metopotoxus, Cochlops, andAsterostemma) were initially recognized in Argentinian Patagonia. Among these,Asterostemma was characterized by its wide latitudinal distribution, ranging from southernmost (Patagonia) to northernmost (Colombia, Venezuela) South America. However, the generic assignation of the Miocene species from Colombia and Venezuela (A.? acostae, A. gigantea, andA. venezolensis) was contested by some authors, who explicitly accepted the possibility that these species could correspond to a new genus, different from those recognized in southern areas. A new comparative study of taxa from Argentinian Patagonia, Colombia and Venezuela (together with the recognition of a new genus and species for the Pliocene of the latter country) indicates that the species in northern South America are not Propalaehoplophorinae, but represent the first stages in the cladogenesis of the Glyptodontinae glyptodontids, the history of which was heretofore restricted to the late Miocene — early Holocene of southernmost South America. Accordingly, we propose the recognition of the new genusBoreostemma for the species from northern South America and the restriction ofAsterostemma to the Miocene of Patagonia. Thus, the available data indicate that the Glyptodontinae would in fact have arisen in the northernmost regions of this continent. Their arrival to more southerly areas coincides with the acme of the “Age of Southern Plains”. The Propalaehoplophorinae are geographically restricted to Patagonia. 相似文献
984.
CORONATINE INSENSITIVE 1 (COI1) is a well-known key player in processes downstream of jasmonic acid (JA) biosynthesis: silencing COI1 in Nicotiana attenuata (ir-coi1) makes plants insensitive to JA, prevents the up-regulation of JA-mediated defenses and decreases the plant's resistance to herbivores and pathogens. In agreement with previous studies, we observed that regulation of several JA biosynthesis genes elicited by Manduca sexta oral secretions (OS) is COI1 dependent. In response to wounding and application of OS ir-coi1 plants accumulate 75% less JA compared with wild-type plants (WT), resembling JA levels found in plants silenced in the key enzyme in JA biosynthesis LIPOXYGENASE 3 (as-lox). However, while OS-elicited as-lox plants also accumulated lower levels of the JA-conjugate JA-isoleucine (JA-Ile) than did WT plants, JA-Ile accumulation in ir-coi1 was higher, prolonged and peaked with a delay of 30 min. In vivo substrate feeding experiments of N. attenuata demonstrate that the increased and prolonged JA-Ile accumulation pattern in ir-coi1 is not the result of altered substrate availability, i.e. of JA and/or Ile, but is due to an approximately 6-fold decrease in JA-Ile turnover. These results provide the first evidence for a second, novel regulatory feedback function of COI1 in enhancing JA-Ile turnover. Hence, in addition to its control over JA biosynthesis, COI1 might fine-tune the dynamics of the jasmonate response after induction by herbivore elicitors. 相似文献
985.
Colon GA 《Plastic and reconstructive surgery》2008,122(3):993; author reply 993
986.
987.
Patricia C lia da Rocha Viana Maria Clara Caldas Bussiere Rachel Siqueira de Queiroz Sim es Marins Luiz Silveira Menna-Barreto Leopoldo Jos Cury 《Biological Rhythm Research》2003,34(4):347-354
The purposes of this study were to observe the presence of diurnal rhythms in plasma ions and metabolites levels in Thoroughbred racehorses under physical training, and to determine the time of blood sampling in clinical investigations. Plasma calcium, phosphorus, potassium, sodium, chloride, magnesium, iron, glucose, cholesterol, triglycerides, and total proteins levels were studied over a 72-h period. Blood samples were taken every 4 hours from five male and five female Thoroughbred racehorses under physical training. COSINOR analyses (P = 0.05) were done. Plasma potassium and triglycerides showed significant diurnal rhythms, with its acrophases occurring at dark period. No significant diurnal rhythms of other variables were found. It was concluded that, in Thoroughbred racehorses, the optimum time for potassium, and triglycerides sampling seems to be light period. And for other variables, time of diagnosis is not important. 相似文献
988.
Juliana Moreira de Sousa-Canavez Cristina de Oliveira Massoco Elaine Cristina Corneta Luiz Heraldo Camara-Lopes 《Cellular immunology》2009,259(1):41-48
All-trans-retinoic acid (atRA) appears to affect Th1-Th2 differentiation and its effects on immune responses might also be mediated by dendritic cell (DC). Nonetheless, studies have been showing contradictory results since was observed either induction or inhibition of DC differentiation. Our aim was to investigate atRA action on human monocyte derived DC differentiation. For this purpose we tested pharmacological and physiological doses of atRA with or without cytokines. Cell phenotypes were analyzed by flow cytometry and function was investigated by phagocytosis and respiratory burst. DC, positive control group, was differentiated with GM-CSF and IL-4 and maturated with TNF-α. We demonstrated that atRA effects depend on the dose used as pharmacological doses inhibited expression of all phenotypic markers tested while a physiological dose caused cell differentiation. However, atRA combined or not with cytokines did not promote DC differentiation. In fact, atRA was detrimental on IL-4 property as a DC inductor. 相似文献
989.
Raúl González Gustavo Ferrín Ana B. Hidalgo Pedro López-Cillero Mónica Santos-Gónzalez Javier Briceño Miguel A. Gómez José M. Villalba Manuel de la Mata Jordi Muntané 《Chemico-biological interactions》2009,181(1):95-755
d-Galactosamine (d-GalN) induces reactive oxygen species (ROS) generation and cell death in cultured hepatocytes. The aim of the study was to evaluate the cytoprotective properties of N-acetylcysteine (NAC), coenzyme Q10 (Q10) and the superoxide dismutase (SOD) mimetic against the mitochondrial dysfunction and cell death in d-GalN-treated hepatocytes. Hepatocytes were isolated from liver resections. NAC (0.5 mM), Q10 (30 μM) or MnTBAP (Mn(III)tetrakis(4-benzoic acid) porphyrin chloride (1 mg/mL) were co-administered with d-GalN (40 mM) in hepatocytes. Cell death, oxidative stress, mitochondrial transmembrane potential (MTP), ATP, mitochondrial oxidized/reduced glutathione (GSH) and Q10 ratios, electronic transport chain (ETC) activity, and nuclear- and mitochondria-encoded expression of complex I subunits were determined in hepatocytes. d-GalN induced a transient increase of mitochondrial hyperpolarization and oxidative stress, followed by an increase of oxidized/reduced GSH and Q10 ratios, mitochondrial dysfunction and cell death in hepatocytes. The cytoprotective properties of NAC supplementation were related to a reduction of ROS generation and oxidized/reduced GSH and Q10 ratios, and a recovery of mitochondrial complexes I + III and II + III activities and cellular ATP content. The co-administration of Q10 or MnTBAP recovered oxidized/reduced GSH ratio, and reduced ROS generation, ETC dysfunction and cell death induced by d-GalN. The cytoprotective properties of studied antioxidants were related to an increase of the protein expression of nuclear- and mitochondrial-encoded subunits of complex I. In conclusion, the co-administration of NAC, Q10 and MnTBAP enhanced the expression of complex I subunits, and reduced ROS production, oxidized/reduced GSH ratio, mitochondrial dysfunction and cell death induced by d-GalN in cultured hepatocytes. 相似文献
990.
Kilian Toledo Guedes Pablo Sánchez-Jerez Gustavo González-Lorenzo Alberto Brito Hernández 《Hydrobiologia》2009,623(1):203-212
This article gives an insight into the degree of establishment and potential impact of escaped sea bass in shallow coastal
habitats of Tenerife. We studied spatial distribution, abundance, size frequency and use of habitat by visual census. Stomach
contents and gonadal maturity of escaped sea bass were analysed. Results show that abundance of escaped sea bass is related
to distance from to the nearest fish farm and to the habitat complexity. The most frequent size found in the wild corresponds
to that of first marketing size. Stomach contents indicate that escaped sea bass were able to exploit natural resources, especially
fish. The presence of a female with mature gonads indicates that conditions for successful maturation exist. However, further
studies are needed to ensure whether or not escaped sea bass are able to establish self-reproducing populations. Thus, we
can conclude that sea bass seem to be already established in shallow coastal habitats of Tenerife, but the population may
depend mainly on escapees. As a result of being an opportunistic piscivore, sea bass could be predating on and competing with
other native species that are economically important. These results are useful to develop a risk assessment based on quantitative
data to predict negative effects. Moreover, management policies such as communication of massive escapes or mitigation measures
are needed to prevent future impact on the marine environment.
Handling editor: P. Viaroli 相似文献