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21.
European Atlantic salmon (Salmo salar) populations inhabit rivers from northern Portugal to northern Norway across a wide spectrum of environmental variability. To address whether single physical factors might lead to genetic divergence of isolated populations, we compared the digestive performances total digestibility, relative nitrogen digestibility, passage time, and digestion rate (g dry matter · h–1) — of northern (Scotland) and southern (Asturias, northern Spain) populations at three temperature regimes (5, 12, and 20° C). Total dry matter digestibilities increased directly with temperature but were similar for both populations at each of the three trials. Relative nitrogen digestibility did not differ between populations nor among temperature regimes. In contrast, passage time was significantly longer for low-than for high-latitude fish at both 5 and 20° C. When the percentage of food digested and the passage time were integrated as digestion rates (food digested per unit time), a significant population × temperature interaction consistent with a genotype × environment interaction was detected in addition to the population and temperature effects. This implies that not only is the digestive performance of the high-latitude population higher throughout the range of temperatures examined, but moreover the difference is reinforced at high temperatures, where the digestion rate of high-latitude fish was 1.6 times greater. Taken together, these two results provide preliminary evidence for countergradient variation in digestive rates of salmonids in response to variation in growth opportunity. The data support our previous work on the same two populations showing differences in growth rates, and underlie one of the possible mechnisms leading to more rapid growth of the high-latitude fish when both populations are reared in a common environment. 相似文献
22.
I. C. Roberto I. M. Mancilha C. A. de Souza M. G. A. Felipe S. Sato H. F. de Castro 《Biotechnology letters》1994,16(11):1211-1216
Summary Rice straw was used as a lignocellulosic source to provide rich pentose media. By using a well characterized yeast strain,Candida guilliermondii FTI 20037, the hydrolysate obtained was converted to xylitol with an efficiency of 75% and production of 27 g of xylitol per liter in 48 hours. The satisfactory results reported here can be attributed to the low concentrations of toxic components generated throughout the chemical depolymerization of this raw material. 相似文献
23.
Maria Ieda Siqueira Linhares Yoshito Eizuru Gildete Patriota de Andrade Ivailda Barbosa Fonseca Luiz Bezerra Carvalho Ivanildo Tenorio Moreira Yoichi Minamishima 《Microbiology and immunology》1994,38(6):475-478
The seroprevalence of human T cell leukemia virus type 1 (HTLV-1) infection was investigated in Brazilians (570): native inhabitants (298) and descendants from Japanese (272) living in Recife and its neighborhoods—North-east of Brazil. Furthermore, polytransfused renal transplanted patients (54) were also examined for the serological status to this virus. The seropositivity to HTLV-1, screened by enzyme-linked immunosorbent assay (ELISA), was low: 1.34% for the local population and 0.73% for the descendants from Japanese. However, the seropositivity for the renal transplanted patients was found to be 11.1%. This higher value suggests that this retrovirus infection seems to be of importance in this clinical condition. 相似文献
24.
Eduardo Moreno-Jiménez Fernando T. Maestre Maren Flagmeier Emilio Guirado Miguel Berdugo Felipe Bastida Marina Dacal Paloma Díaz-Martínez Raúl Ochoa-Hueso César Plaza Matthias C. Rillig Thomas W. Crowther Manuel Delgado-Baquerizo 《Global Change Biology》2023,29(2):522-532
Soil micronutrients are capital for the delivery of ecosystem functioning and food provision worldwide. Yet, despite their importance, the global biogeography and ecological drivers of soil micronutrients remain virtually unknown, limiting our capacity to anticipate abrupt unexpected changes in soil micronutrients in the face of climate change. Here, we analyzed >1300 topsoil samples to examine the global distribution of six metallic micronutrients (Cu, Fe, Mn, Zn, Co and Ni) across all continents, climates and vegetation types. We found that warmer arid and tropical ecosystems, present in the least developed countries, sustain the lowest contents of multiple soil micronutrients. We further provide evidence that temperature increases may potentially result in abrupt and simultaneous reductions in the content of multiple soil micronutrients when a temperature threshold of 12–14°C is crossed, which may be occurring on 3% of the planet over the next century. Altogether, our findings provide fundamental understanding of the global distribution of soil micronutrients, with direct implications for the maintenance of ecosystem functioning, rangeland management and food production in the warmest and poorest regions of the planet. 相似文献
25.
Elnur Babayev Chanakarn Suebthawinkul Dilan Gokyer Wendena S. Parkes Felipe Rivas Mary Ellen Pavone Adam R. Hall Michele T. Pritchard Francesca E. Duncan 《Aging cell》2023,22(11):e14004
Reproductive aging is associated with ovulatory defects. Age-related ovarian fibrosis partially contributes to this phenotype as short-term treatment with anti-fibrotic compounds improves ovulation in reproductively old mice. However, age-dependent changes that are intrinsic to the follicle may also be relevant. In this study, we used a mouse model to demonstrate that reproductive aging is associated with impaired cumulus expansion which is accompanied by altered morphokinetic behavior of cumulus cells as assessed by time-lapse microscopy. The extracellular matrix integrity of expanded cumulus–oocyte complexes is compromised with advanced age as evidenced by increased penetration of fluorescent nanoparticles in a particle exclusion assay and larger open spaces on scanning electron microscopy. Reduced hyaluronan (HA) levels, decreased expression of genes encoding HA-associated proteins (e.g., Ptx3 and Tnfaip6), and increased expression of inflammatory genes and matrix metalloproteinases underlie this loss of matrix integrity. Importantly, HA levels are decreased with age in follicular fluid of women, indicative of conserved reproductive aging mechanisms. These findings provide novel mechanistic insights into how defects in cumulus expansion contribute to age-related infertility and may serve as a target to extend reproductive longevity. 相似文献
26.
Paula Tatiana Lopes Seixas Antonio Jacinto Demuner Luiz Claudio Almeida Barbosa Cristiane Isaac Cerceau Daiane Einhardt Blank Marcelo Henrique Dos Santos Elizeu de Sá Farias Marcelo Coutinho Picanço 《Journal of Applied Entomology》2023,147(2):116-125
Aedes aegypti L. (Diptera: Culicidae) is a vector for serious diseases in tropical regions. This pest is mainly controlled by commercial larvicides but the application of such products has led to environmental problems. Essential oils (EO) have been consistently reported as molecules with insecticidal activity and can be used to produce more environmentally friendly larvicides in the control of A. aegypti. In this study, the larvicidal effect of essential oils (EO) from the leaves of three Artemisia species was evaluated against A. aegypti. The oils were obtained from steam distillation and their chemical composition was determined by gas chromatography–mass spectrometry. The EO of Artemisia camphorata was the most active in the screening bioassay and presented LC50 and LC95 of 64.95 and 74.18 μg ml−1, respectively. In addition, we found that germacrene D-4-ol was the constituent responsible for the toxicity of this EO. Artemisia camphorata EO and its major constituent, germacrene D-4-ol, are promising for the development of natural larvicides against A. aegypti. 相似文献
27.
Norihiko Misawa Shigeyuki Yamano Hartmut Linden Maria R. de Felipe Mercedes Lucas Hiroshi Ikenaga Gerhard Sandmann 《The Plant journal : for cell and molecular biology》1993,4(5):833-840
Among the enzymes involved in carotenoid biosynthesis, phytoene desaturase is considered to be a rate-limiting enzyme in this pathway and is also the target of many bleaching herbicides. This enzyme shows diversity concerning its function and amino acid homology among various organisms. The phytoene desaturase gene crtl of Erwinia uredovora was expressed, the 5'-region of which was fused to the sequence for the transit peptide of a pea Rubisco small subunit, in tobacco plants under the control of the CaMV 35S promoter. This chimeric gene product was targeted into chloroplasts and processed in the transgenic plants. The production and processing of the corresponding protein could be demonstrated by Western blotting. Immunogold localization showed that the location of the gene product Crtl was preferentially in the thylakoids. A radioactive labeling study using the leaves demonstrated enhanced activity for the synthesis of β-carotene. In addition, the transgenic tobacco acquired elevated resistance to the bleaching herbicide norflurazon. 相似文献
28.
29.
Barlette Vania Elisabeth Garbujo Fábio Luiz Laurenti Freitas Luiz Carlos Gomide 《Molecular Engineering》1997,7(3-4):439-455
A five site potential model combining Lennard–Jones plus Coulomb potential functions has been developed for chloroform molecule.
The partial charges needed for Coulombic interactions were derived using the chelpg procedure implemented in the gaussian
92 program. These calculations were performed at the MP2 level with MC-311G* basis set for Cl and 6-311G** for C and H atoms.
The parameters for the Lennard–Jones potentials were optimized to reproduce experimental values for the density and enthalpy
of vaporization of the pure liquid at 298 K and 1 atm. The statistical mechanics calculations were performed with the Monte
Carlo method in the isothermic and isobaric (NpT) ensemble. Besides the values obtained for density, ρ, and molar enthalpy
of vaporization at constant pressure, Δ HV, for liquid chloroform, results for molar volume, Vm, molar heat capacity, Cp, isobaric thermal expansivity, αp, and isothermal compressibility, κT, for this pure liquid are also in very good agreement with experimental observations. Size effects on the values of thermodynamic
properties were investigated. The potential model was also tested by computing the free energy for solvating one chloroform
molecule into its own liquid at 298 K using a statistical perturbation approach. The result obtained compares well with the
experimental value. Site–site pair correlation functions were calculated and are in good accordance with theoretical results
available in the literature. Dipole–dipole correlation functions for the present five site model were also calculated at different
carbon–carbon distances. These correlations were compared to those obtained using the four site model reported in the literature.
An investigation of the solvent dependence of the relative free energy for cis/trans conversion of a hypothetical solute in
TIP4P water and chloroform was accomplished. The results show strong interaction of water and chloroform molecules with the
gauche conformer. The value obtained for the free energy barrier for cis/trans rotation in TIP4P water is higher than that
for chloroform. This result is in agreement with the continuous theory for solvation as the conformer with higher dipole moment
is more favoured by the solvent with higher dieletric constant. The results also show an increase in entropy as the solute
goes from the cis to the trans geometry and this result is more appreciable in the aqueous solution.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
30.
Dinucleotide repeat polymorphism at the D4S2458 locus close to the PKD2 locus on human chromosome 4q
Miguel Viribay Dolores Tellería Eladio Velasco Felipe Moreno José L. San Millán 《Human genetics》1995,95(5):601-602
A new polymorphic CA repeat sequence was identified within the candidate region fot the autosomal dominant polycystic kidney disease type 2 (PKD2) locus. It should be a useful marker in the localization of this gene. 相似文献