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41.
GU
BJ
RG .
LAFSD
TTIR SIGUR
UR S. SNORRASON 《Biological journal of the Linnean Society. Linnean Society of London》2009,98(4):803-813
The frequent occurrence of parallel phenotypic divergence in similar habitats is often evoked when emphasizing the role of ecology in adaptive radiation and speciation. However, because phenotypic plasticity can contribute to the observed pattern of divergence, confirmation of divergence at loci underlying phenotypic traits is important for confirming adaptive divergence. In the present study, we examine parallel morphological, neutral, and potentially adaptive genetic divergence of threespine stickleback inhabiting different habitats within a lake. Three genetic clusters best explained the neutral genetic structure within the lake; however, morphological differences were only weakly connected to genetic clusters and there was considerable phenotypic variation within clusters. Among the factors that could contribute to the observed pattern of morphological and genetic divergence are phenotypic plasticity, selective mortality of hybrids, and habitat choice based on morphology. Several loci are identified as outliers indicating divergent selection between the morphs and some parallels in morphological and adaptive genetic divergence are found in stickleback spawning at two lava sites. However, neutral genetic structure indicates considerable genetic connectivity among the two lava sites, and the parallels in morphology may therefore represent selective distribution of phenotypes rather than parallel divergence. © 2009 The Linnean Society of London, Biological Journal of the Linnean Society, 2009, 98 , 803–813. 相似文献
42.
CHRISTOPH OBERPRIELER JÖRG MEISTER CHRISTINE SCHNEIDER NORBERT KILIAN 《Biological journal of the Linnean Society. Linnean Society of London》2009,97(1):40-51
Anogeissus dhofarica (Combretaceae) is an endemic tree of the monsoon affected coastal mountains of the southern Arabian Peninsula, being the character species of the Hybantho durae–Anogeissetum dhofaricae association, a drought deciduous, monsoon forest community found only in the Dhofar region of southern Oman and the eastern Al‐Mahra region of south‐east Yemen. Due to the steep precipitation gradient from the centre to the edges in this monsoon affected area, A. dhofarica is found in two different habitat types: in continuous woodland belts of the Hawf and Dhofar mountains, and in isolated, scattered woodland patches, as found especially in the Fartak Mts (south‐east Yemen). Fifteen populations (212 individuals) from across the whole distribution area of the species were analysed using amplified fragment length polymorphism fingerprinting to: (1) evaluate the consequences of population fragmentation on the genetic diversity harboured in isolated patches versus cohering stands of the species and (2) to reconstruct the phylogeographical pattern of A. dhofarica as a consequence of oscillations in the monsoon activity during the Pleistocene and Holocene. The analysis of among‐population genetic differentiation and within‐population genetic diversity in A. dhofarica populations resulted in a lack of genetic pauperization and genetic differentiation of populations of the distinctly isolated patches of the Fartak Mts compared to the more luxurious forests of the Hawf and Dhofar regions. This is considered to be due to the high buffer capacity against the loss of genetic diversity caused by the long‐lived life‐form of the species combined with the capability to propagate clonally and the relatively recent fragmentation of Anogeissus forests into the described patches rather than due to high values of gene flow among remnant populations caused by bee pollination and anemochorical and hydrochorical diaspore dispersal. The phylogeographical pattern of the species argues for a quite recent fragmentation of a once continuous forest belt of A. dhofarica that is rather connected with climate changes in the Holocene than triggered by aridity–humidity oscillations reported for the Pleistocene. © 2009 The Linnean Society of London, Biological Journal of the Linnean Society, 2009, 97 , 40–51. 相似文献
43.
JÖRG MALETZ 《Palaeontology》2010,53(2):415-439
Abstract: The virgellar spine is one of the most consistent features of the graptolite sicula. It is present in a large number of graptoloid groups, but evolved separately and independently in these as it is seen from the presence of the spine in either ventral (Axonophora) or dorsal (Phyllograptus, Xiphograptus) position. The evolution of the virgellar spine in the Pan‐Bireclinata in the Upper Dapingian to Lower Darriwilian time interval is known to follow four main steps, from a simple rutellum, through a lamelliform rutellum and a lanceolate virgella to the true virgellar spine. For the xiphograptids and in Phyllograptus, the origin and early development is less well documented, but appears to follow a similar path. However, the individual stages are condensed, and a true virgellar spine emerges already in the Floian time interval. A virgellar spine was found in Didymograptellus bifidus, necessitating a revision of the diagnosis of the genus Didymograptellus. A number of species of the virgellate genera Xiphograptus, Yutagraptus and Didymograptellus are described from isolated material for the first time. The species are useful for the biostratigraphic correlation of endemic mid‐continent North American faunas with the Pacific Type faunal realm. Xiphograptus artus sp. nov., Didymograptellus primus sp. nov. and Didymograptellus cowheadensis sp. nov. from the Cow Head Group of western Newfoundland are described as new. 相似文献
44.
45.
Twenty‐seven polymorphic microsatellite markers were isolated from red clover (Trifolium pratense). Allelic variability and cross‐species amplification were assessed on 24 red clover and eight white clover (Trifolium repens) genotypes. The number of alleles detected in red clover ranged from two to 25. Observed and expected heterozygosities were high with average values of 0.71 and 0.88, respectively. Five of the 27 loci were also successfully amplified from white clover, where two to 13 alleles were detected. These highly polymorphic microsatellite loci provide powerful tools for population genetic studies as well as for marker‐assisted selection in this important forage legume species. 相似文献
46.
Tramper J Battershill C Brandenburg W Burgess G Hill R Luiten E Müller W Osinga R Rorrer G Tredici M Uriz M Wright P Wijffels R 《Biomolecular engineering》2003,20(4-6):467-471
During the symposium "Marine Biotechnology: Basics and Applications", held 25 February-1 March, 2003 in Matalasca?as, Spain, a special brainstorm session was organized. Two questions were addressed: 1, "What is the most desirable development in marine biotechnology"?; 2, "What is the most spectacular development in this field in your 'wildest' dreams"? The outcome of this session is reported in this paper. From the more than 250 ideas generated, concern for the environment and human health emerged as the most significant issues. 相似文献
47.
Noordover JA Hofmeester JJ van der Burg JP de Leeuw A van Dijck PW Luiten RG Groot GS 《Journal of industrial microbiology & biotechnology》2002,28(2):65-69
Both physical and biological containment are considered to be essential parts in the risk analysis of industrial Good Industrial
Large-Scale Practice (GILSP) processes using genetically modified organisms (GMOs). Biological containment of industrial microorganisms
has become a more important issue since the introduction of recombinant DNA techniques. In the event of an accidental discharge
in the production plant, a large amount of organisms could be released into the wastewater treatment (WWT) system. This WWT
system should therefore be considered as a part of the containment. This study demonstrates both a hydrodynamic and a microbiological
model for the containment aspects of industrial WWT plants. The models are verified by measurements using industrial hosts
of GILSP GMOs at full scale. Both models describe the full-scale equipment accurately. The results are supplemented with microcosm
studies on survival of GMOs in defined niches. It is shown that WWT plants can be considered as useful additional parts of
the containment of microorganisms, in case of an accidental discharge. The effect of drainage of an enormous amount of microorganisms
(several tons) through the WWT plant into the environment is shown to be comparable to the direct drainage of a small-scale
fermenter. Microcosm experiments correlate well with the survival rates in the WWT and therefore can be of use to predict
the behaviour of GMOs in this environment. Journal of Industrial Microbiology & Biotechnology (2002) 28, 65–69 DOI: 10.1038/sj/jim/7000210
Received 16 July 2001/ Accepted in revised form 05 September 2001 相似文献
48.
ERWIN REINWALD CLAUDIA HEIDRICH HANS-J
RG RISSE 《The Journal of eukaryotic microbiology》1984,31(2):300-306
Tritiated leucine, glucosamine, mannose, and galactose were incorporated into the variant specific surface glycoprotein (VSG) of Trypanosoma congolense in vitro. The uptake of the precursors is shown by SDS-polyacrylamide electrophoresis and fluorography, by assay of the radioactivity in immunoprecipitates obtained with specific antisera, and by the isolation of the labeled antigens by affinity chromatography on concanavalin A-sepharose and isoelectric focusing. The in vitro labeled VSG exhibits the same degree of microheterogeneity as that observed in the VSG isolated from trypanosomes grown in animals. Analysis of the incorporated sugars after hydrolysis of the glycoprotein showed that glucosamine and mannose were utilized in biosynthesis of the carbohydrate moiety directly whereas galactose was converted possibly to other intermediates before being incorporated into the antigen. Tunicamycin completely prevented the incorporation of the radiolabeled sugars into the surface glycoprotein. The unglycosylated VSG with a molecular weight of 47 kDa had completely lost its size heterogeneity. 相似文献
49.
Streptomyces clavuligerus is a commercially important actinomycete that is used to produce clavulanic acid, a beta-lactamase inhibitor. Observations during 10 batch cultivations with S. clavuligerus on defined media have led to the finding that the organism is very sensitive to shear when grown in batch cultures with increasing stirrer speed. The stirrer speed was increased to keep the dissolved oxygen level above 50% air saturation. A quantitative approach based on the calculation of elemental balances and a simple mathematical model is proposed to characterize the biomass lysis. Finally, a linear relation between biomass yield and observed specific growth rate is determined. Results show that cell lysis occurs at a high degradation rate, e.g., mu(max) = 0.16 h(-1) and k(d) = 0.07 h(-1), when the gassed power input increases above 1.1, 1.7, or 2.0 kW/m(3), respectively, depending on the medium composition. The overall biomass yield on substrate is dramatically reduced in all experiments (>30%). 相似文献
50.
A serum-free medium that supports the growth of cultured skeletal muscle satellite cells 总被引:2,自引:0,他引:2
Ronald E. Allen Michael V. Dodson Lynda S. Luiten Linda K. Boxhorn 《In vitro cellular & developmental biology. Plant》1985,21(11):636-640
Summary A serum-free medium has been devised that supports the proliferation and differentiation of primary cultures of rat skeletal
muscle satellite cells for up to 4 d. The medium consists of a mixture of Dulbecco's modified Eagle's medium and MCDB-104
plus insulin, dexamethasone, pituitary fibroblast growth factor, Deutsch fetuin, and linoleic acid. In addition to promoting
the formation of myotubes from satellite cells, a decrease in fibroblast contamination of these cultures was observed when
cultures grown in serum-free medium were compared to cultures grown in serum-containing medium.
This work was supported by the Arizona Agriculture Experiment Station, Project No. R11, U.S. Public Health Service Grant R01
AG03393, Lilly Research Laboratoires, and Merck Institute for Therapeutic Research. This communication is Arizona Agriculture
Experiment Station Journal Paper No. 3966. 相似文献