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951.
An exploration of the role of Sertoli cells on fetal testis development using cell ablation strategy
Yu‐Qian Wang Jin‐Mei Cheng Qing Wen Ji‐Xin Tang Jian Li Su‐Ren Chen Yi‐Xun Liu 《Molecular reproduction and development》2020,87(2):223-230
Sertoli cells (SCs) are presumed to be the center of testis differentiation because they provide both structural support and biological regulation for spermatogenesis. Previous studies suggest that SCs control germ cell (GC) count and Leydig cell (LC) development in mouse testes. However, the regulatory role of SCs on peritubular myoid (PTM) cell fate in fetal testis has not been clearly reported. Here, we employed Amh‐Cre; diphtheria toxin fragment A (DTA) mouse model to selectively ablate SCs from embryonic day (E) 14.5. Results found that SC ablation in the fetal stage caused the disruption of testis cords and the massive loss of GCs. Furthermore, the number of α‐smooth muscle actin‐labeled PTM cells was gradually decreased from E14.5 and almost lost at E18.5 in SC ablation testis. Interestingly, some Ki67 and 3β‐HSD double‐positive fetal LCs could be observed in Amh‐Cre; DTA testes at E16.5 and E18.5. Consistent with this phenomenon, the messenger RNA levels of Hsd3b1, Cyp11a1, Lhr, Star and the protein levels of 3β‐HSD and P450Scc were significantly elevated by SC ablation. SC ablation appears to induce ectopic proliferation of fetal LCs although the total LC number appeared reduced. Together, these findings bring us a better understanding of SCs’ central role in fetal testis development. 相似文献
952.
953.
Jin Heyan Yu Yang Hu Shijie Quan Xueli Wu Songquan 《In vitro cellular & developmental biology. Plant》2020,56(4):407-414
In Vitro Cellular & Developmental Biology - Plant - Calycosin-7-O-β-D-glucoside (CG), a methoxylated isoflavonoid in Astragalus membranaceus Fisch. (Bunge), has a wide range of biological... 相似文献
954.
Abramov O. N. Aleksandrov V. V. Volkov G. S. Volobuev I. V. Grabovsky E. V. Gritsuk A. N. Zhigalov D. V. Kalinin Yu. G. Korolev V. D. Laukhin Ya. N. Medovshchikov S. F. Mitrofanov K. N. Oleinik G. M. Smirnova E. A. Frolov I. N. 《Plasma Physics Reports》2020,46(10):967-977
Plasma Physics Reports - Compression of a fiber array with a deuterated target mounted on its axis is studied at the Angara-5-1 facility (3.5 MA, 100 ns). Cylindrical arrays with an initial... 相似文献
955.
Artem’ev K. V. Batanov G. M. Berezhetskaya N. K. Borzosekov V. D. Gritsinin S. I. Davydov A. M. Kolik L. V. Konchekov E. M. Kossyi I. A. Lebedev Yu. A. Moryakov I. V. Petrov A. E. Sarksyan K. A. Stepakhin V. D. Kharchev N. K. Shakhatov V. A. 《Plasma Physics Reports》2020,46(3):311-319
Plasma Physics Reports - A subthreshold discharge excited by a microwave beam in air at pressures close to atmospheric is studied as a plasmachemical method of nitrogen oxide (NOx) production. It... 相似文献
956.
Popov A. Yu. Tretinnikov P. V. Gusakov E. Z. Simonchik L. V. 《Plasma Physics Reports》2020,46(1):35-40
Plasma Physics Reports - The expression is obtained for the high-frequency quadratic susceptibility of the strongly inhomogeneous magnetoactive plasma, which describes the nonlinear coupling... 相似文献
957.
Russian Journal of Marine Biology - The dynamics of the Atka mackerel stock in the Olyutorsky–Navarin area in 1994–2019 is inferred from bottom-trawl surveys, fishery statistics,... 相似文献
958.
Ming‐Yuan Du Jia‐Xi Duan Chen‐Yu Zhang Hui‐Hui Yang Xin‐Xin Guan Wen‐Jing Zhong Yan‐Zhe Liu Zi‐Ming Li Yu‐Rui Cheng Yong Zhou Cha‐Xiang Guan 《Cell biology international》2020,44(1):98-107
Idiopathic pulmonary fibrosis (IPF) is a progressive disease characterized by excessive deposition of extracellular matrix (ECM) and chronic inflammation with limited therapeutic options. Psoralen, a major active component extracted from Psoralea corylifolia L. seed, has several biological effects. However, the role of psoralen in IPF is still unclear. Here, we hypothesized that psoralen played an essential role in IPF in the inhibition of fibroblast proliferation and inflammatory response. A murine model of IPF was established by injecting bleomycin (BLM) intratracheally, and psoralen was administered for 14 days from the 7th to 21st day after BLM injection. Our results demonstrated that psoralen treatment reduced body weight loss and improved the survival rate of mice with IPF. Histological and immunofluorescent examination showed that psoralen alleviated BLM‐induced lung parenchymal inflammatory and fibrotic alteration. Furthermore, psoralen inhibited proliferation and collagen synthesis of mouse fibroblasts and partially reversed BLM‐induced expression of α‐smooth muscle actin at both the tissue and cell level. Moreover, psoralen decreased the expression of transforming growth factor‐β1, interleukin‐1β, and tumor necrosis factor‐α in the lungs of BLM‐stimulated mice. Our results reveale for the first time that psoralen exerts therapeutic effects against IPF in a BLM‐induced murine model. 相似文献
959.
Xiao‐Li Li Fei Yu Bao‐Ying Li Chun‐Li Fu Xin Yu Mei Xu Mei Cheng Hai‐Qing Gao 《Cell biology international》2020,44(1):268-277
The progression of diabetic cardiomyopathy is related to cardiomyocyte dysfunction and apoptosis. Our previous studies showed that asporin (ASPN) was significantly increased in the myocardium of db/db mice through proteomics, and grape seed procyanidin B2 (GSPB2) significantly inhibited the expression of ASPN in the heart of db/db mice. We report here that ASPN played a critical role in glycated low‐density lipoproteins (gly‐LDL) induced‐cardiomyocyte apoptosis. We found that gly‐LDL upregulated ASPN expression. ASPN increased H9C2 cardiomyocyte apoptosis with down‐regulation of Bcl‐2, upregulation of transforming growth factor‐β1, Bax, collagen III, fibronectin, and phosphorylation of smad2 and smad3. However, GSPB2 treatment reversed ASPN‐induced impairments in H9C2 cardiomyocytes. These results provide evidence for the cardioprotective action of GSPB2 against ASPN injury, and thus suggest a new target for fighting against diabetic cardiomyopathy. 相似文献
960.
Russian Journal of Plant Physiology - Organisms can produce heat shock proteins (HSPs) in response to elevated temperatures and other abiotic stresses. However, the function of HSPs, including... 相似文献