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981.
Luis A. Diaz Sandra E. Go?i Javier A. Iserte Agustín I. Quaglia Amber Singh Christopher H. Logue Ann M. Powers Marta S. Contigiani 《PloS one》2015,10(8)
St. Louis encephalitis virus (SLEV) is a re-emerging arbovirus in South America. In 2005, an encephalitis outbreak caused by SLEV was reported in Argentina. The reason for the outbreak remains unknown, but may have been related to virological factors, changes in vectors populations, avian amplifying hosts, and/or environmental conditions. The main goal of this study was to characterize the complete genome of epidemic and non-epidemic SLEV strains from Argentina. Seventeen amino acid changes were detected; ten were non-conservative and located in proteins E, NS1, NS3 and NS5. Phylogenetic analysis showed two major clades based on geography: the North America and northern Central America (NAnCA) clade and the South America and southern Central America (SAsCA) clade. Interestingly, the presence of SAsCA genotype V SLEV strains in the NAnCA clade was reported in California, Florida and Texas, overlapping with known bird migration flyways. This work represents the first step in understanding the molecular mechanisms underlying virulence and biological variation among SLEV strains. 相似文献
982.
983.
B. Rivero-Gutiérrez A. Anzola O. Martínez-Augustin F. Sánchez de Medina 《Analytical biochemistry》2014
It is currently a routine practice to require a measurement of a housekeeping reference, including actin, glyceraldehyde-3-phosphate dehydrogenase, β-tubulin, among others, in Western blots, as it is the rule in RNA blots. Reversible Ponceau staining has been applied successfully to check equal loading of gels. Here we test a new technique, with the Stain-Free gels from Bio-Rad, against both Ponceau staining and housekeeping protein immunodetection under different conditions. Our results show that Stain-Free gels outperform Ponceau staining and that both are more consistent than housekeeping proteins as a loading control. 相似文献
984.
985.
986.
The leader protease (Lpro) from foot-and-mouth disease virus (FMDV) has the ability to cleave eIF4G, leading to a blockade of cellular protein synthesis. In contrast to previous reports, our present findings demonstrate that FMDV Lpro is able to increase translation driven by FMDV IRES. Additionally, inactivation of eIF2 subsequent to phosphorylation induced by arsenite or thapsigargin in BHK cells blocks protein synthesis directed by FMDV IRES, whereas in the presence of Lpro, significant translation is found under these conditions. This phenomenon was also observed in cell-free systems after induction of eIF2 phosphorylation by addition of poly(I:C). 相似文献
987.
Guillermo Blanco Luis M. Bautista Dámaso Hornero‐Méndez Sergio A. Lambertucci Guillermo Wiemeyer José A. Sanchez‐Zapata Fernando Hiraldo José A. Donázar 《Ibis》2014,156(3):668-675
Because large species ingest proportionally less food than small ones, it may be predicted that they should incorporate relatively fewer carotenoids to a proportionally equal volume of blood. However, some species may increase their levels of circulating carotenoids by ingesting unusual food. We tested whether the plasma concentration of carotenoids scales to the three‐quarter power of mass in nine predatory and scavenger raptor species. No significant allometric relationships were found due to the unusually high concentrations of carotenoids in the Egyptian Vulture Neophron percnopterus and the Andean condor Vultur gryphus. To assess whether these two species deviate from the allometric rule through the exploitation of unusual sources of carotenoids, or due to a physiological adaptation to improve the uptake of carotenoids, we determined allometric patterns in individuals of these two species kept in captivity with an exclusive diet of flesh. Our results provided support for the allometric rule because the slope of the allometric equation did not differ from a three‐quarter exponent when carotenoid levels of the two outliers were replaced by those of captive birds. This adjustment to the allometric rule suggests a lack of any physiological adaptation to improve the uptake of the low concentrations of carotenoids contained in flesh. Differences between species in carotenoid incorporation into the bloodstream may be ultimately due to contrasting evolutionary history, physiology and associated colour‐signalling strategies, but proximately due to the acquisition of these micronutrients from both usual and unusual dietary sources. 相似文献
988.
Laura Padilla Sheila DakhelJose Luis Hernández 《Biochemical and biophysical research communications》2014
Secreted by tumor and stromal cells, S100 proteins exert their biological functions via the interaction with surface receptors. The most described receptor is the receptor for advanced glycation end-products (RAGE), thereby participating in the S100-dependent cell migration, invasion, tumor growth, angiogenesis and metastasis. Several approaches have been described for determining this interaction. Here we describe an easy, specific and highly reproducible ELISA-based method, by optimizing several parameters such as the binding and blocking buffer, interaction time and concentrations, directed to screen chemical and biological inhibitors of this interaction for S100A4, S100A7 and S100P proteins. The efficiency of the protocol was validated by using well described neutralizing agents of the RAGE receptor and of the S100A4 activity. The methodology described here will allow future works with other members of the S100 protein family and their receptors. 相似文献
989.
Casimiro Cárdenas Ana R. Quesada Miguel Ángel Medina 《Biochemical and biophysical research communications》2014
The present study aims to identify the modulatory effects of kahweol, an antioxidant diterpene present in coffee beans, on a panel of human tumor cell lines. Kahweol inhibits tumor cell proliferation and clonogenicity and induces apoptosis in several kinds of human tumor cells. In the estrogen receptor-negative MDA-MB231 human breast cancer, the mentioned effects are accompanied by caspases 3/7 and 9 activation and cytochrome c release. On the other hand, kahweol increases the production of reactive oxygen species and their cytotoxicity in human breast cancer cells but not in normal cells. Taken together, our data suggest that kahweol is an antitumor compound with inhibitory effects on tumor cell growth and survival, especially against MDA-MB231 breast cancer cells. 相似文献
990.
Dove Keith Liam Finlay Judy Butler Luis Gómez Eric Smith Régis Moreau Tory Hagen 《Biochemical and biophysical research communications》2014
It is well established that lipid metabolism is controlled, in part, by circadian clocks. However, circadian clocks lose temporal precision with age and correlates with elevated incidence in dyslipidemia and metabolic syndrome in older adults. Because our lab has shown that lipoic acid (LA) improves lipid homeostasis in aged animals, we hypothesized that LA affects the circadian clock to achieve these results. We fed 24 month old male F344 rats a diet supplemented with 0.2% (w/w) LA for 2 weeks prior to sacrifice and quantified hepatic circadian clock protein levels and clock-controlled lipid metabolic enzymes. LA treatment caused a significant phase-shift in the expression patterns of the circadian clock proteins Period (Per) 2, Brain and Muscle Arnt-Like1 (BMAL1), and Reverse Erythroblastosis virus (Rev-erb) β without altering the amplitude of protein levels during the light phase of the day. LA also significantly altered the oscillatory patterns of clock-controlled proteins associated with lipid metabolism. The level of peroxisome proliferator-activated receptor (PPAR) α was significantly increased and acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS) were both significantly reduced, suggesting that the LA-supplemented aged animals are in a catabolic state. We conclude that LA remediates some of the dyslipidemic processes associated with advanced age, and this mechanism may be at least partially through entrainment of circadian clocks. 相似文献