全文获取类型
收费全文 | 11004篇 |
免费 | 802篇 |
出版年
2023年 | 55篇 |
2022年 | 124篇 |
2021年 | 287篇 |
2020年 | 176篇 |
2019年 | 239篇 |
2018年 | 282篇 |
2017年 | 264篇 |
2016年 | 380篇 |
2015年 | 616篇 |
2014年 | 619篇 |
2013年 | 785篇 |
2012年 | 937篇 |
2011年 | 834篇 |
2010年 | 593篇 |
2009年 | 550篇 |
2008年 | 626篇 |
2007年 | 622篇 |
2006年 | 594篇 |
2005年 | 548篇 |
2004年 | 522篇 |
2003年 | 451篇 |
2002年 | 461篇 |
2001年 | 106篇 |
2000年 | 67篇 |
1999年 | 99篇 |
1998年 | 122篇 |
1997年 | 74篇 |
1996年 | 64篇 |
1995年 | 60篇 |
1994年 | 66篇 |
1993年 | 55篇 |
1992年 | 46篇 |
1991年 | 34篇 |
1990年 | 42篇 |
1989年 | 28篇 |
1988年 | 30篇 |
1987年 | 24篇 |
1986年 | 20篇 |
1985年 | 28篇 |
1984年 | 35篇 |
1983年 | 25篇 |
1982年 | 26篇 |
1981年 | 30篇 |
1980年 | 19篇 |
1979年 | 19篇 |
1977年 | 12篇 |
1976年 | 10篇 |
1975年 | 17篇 |
1973年 | 14篇 |
1970年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
971.
972.
973.
974.
María Saura Almudena Fernández Luis Varona Ana I Fernández Maria ángeles R de Cara Carmen Barragán Beatriz Villanueva 《遗传、选种与进化》2015,47(1)
Background
The current availability of genotypes for very large numbers of single nucleotide polymorphisms (SNPs) is leading to more accurate estimates of inbreeding coefficients and more detailed approaches for detecting inbreeding depression. In the present study, genome-wide information was used to detect inbreeding depression for two reproductive traits (total number of piglets born and number of piglets born alive) in an ancient strain of Iberian pigs (the Guadyerbas strain) that is currently under serious danger of extinction.Methods
A total of 109 sows with phenotypic records were genotyped with the PorcineSNP60 BeadChip v1. Inbreeding depression was estimated using a bivariate animal model in which the inbreeding coefficient was included as a covariate. We used two different measures of genomic inbreeding to perform the analyses: inbreeding estimated on a SNP-by-SNP basis and inbreeding estimated from runs of homozygosity. We also performed the analyses using pedigree-based inbreeding.Results
Significant inbreeding depression was detected for both traits using all three measures of inbreeding. Genome-wide information allowed us to identify one region on chromosome 13 associated with inbreeding depression. This region spans from 27 to 54 Mb and overlaps with a previously detected quantitative trait locus and includes the inter-alpha-trypsin inhibitor gene cluster that is involved with embryo implantation.Conclusions
Our results highlight the value of high-density SNP genotyping for providing new insights on where genes causing inbreeding depression are located in the genome. Genomic measures of inbreeding obtained on a SNP-by-SNP basis or those based on the presence/absence of runs of homozygosity represent a suitable alternative to pedigree-based measures to detect inbreeding depression, and a useful tool for mapping studies. To our knowledge, this is the first study in domesticated animals using the SNP-by-SNP inbreeding coefficient to map specific regions within chromosomes associated with inbreeding depression.Electronic supplementary material
The online version of this article (doi:10.1186/s12711-014-0081-5) contains supplementary material, which is available to authorized users. 相似文献975.
Luis Arias-Darraz Deny Cabezas Charlotte K. Colenso Melissa Alegría-Arcos Felipe Bravo-Moraga Ignacio Varas-Concha Daniel E. Almonacid Rodolfo Madrid Sebastian Brauchi 《The Plant cell》2015,27(1):177-188
Sensory modalities are essential for navigating through an ever-changing environment. From insects to mammals, transient receptor potential (TRP) channels are known mediators for cellular sensing. Chlamydomonas reinhardtii is a motile single-celled freshwater green alga that is guided by photosensory, mechanosensory, and chemosensory cues. In this type of alga, sensory input is first detected by membrane receptors located in the cell body and then transduced to the beating cilia by membrane depolarization. Although TRP channels seem to be absent in plants, C. reinhardtii possesses genomic sequences encoding TRP proteins. Here, we describe the cloning and characterization of a C. reinhardtii version of a TRP channel sharing key features present in mammalian TRP channels associated with sensory transduction. In silico sequence-structure analysis unveiled the modular design of TRP channels, and electrophysiological experiments conducted on Human Embryonic Kidney-293T cells expressing the Cr-TRP1 clone showed that many of the core functional features of metazoan TRP channels are present in Cr-TRP1, suggesting that basic TRP channel gating characteristics evolved early in the history of eukaryotes. 相似文献
976.
Superoxide Anion Production and Expression of gp91phox and p47phox Are Increased in Glomeruli and Proximal Tubules of Cisplatin‐Treated Rats
下载免费PDF全文
![点击此处可从《Journal of biochemical and molecular toxicology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Joyce Trujillo Eduardo Molina‐Jijón Omar Noel Medina‐Campos Rafael Rodríguez‐Muñoz José Luis Reyes Diana Barrera José Pedraza‐Chaverri 《Journal of biochemical and molecular toxicology》2015,29(4):149-156
The chemotherapeutic drug cisplatin has some side effects including nephrotoxicity that has been associated with reactive oxygen species production, particularly superoxide anion. The major source of superoxide anion is nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase. However, the specific segment of the nephron in which superoxide anion is produced has not been identified. Rats were sacrificed 72 h after cisplatin injection (7.5 mg/kg), and kidneys were obtained to isolate glomeruli and proximal and distal tubules. Cisplatin induced superoxide anion production in glomeruli and proximal tubules but not in distal tubules. This enhanced superoxide anion production was prevented by diphenylene iodonium, an inhibitor of NADPH oxidase. Consistently, this effect was associated with the increased expression of gp91phox and p47phox, subunits of NADPH oxidase. The enhanced superoxide anion production in glomeruli and proximal tubules, associated with the increased expression of gp91phox and p47phox, is involved in the oxidative stress in cisplatin‐induced nephrotoxicity. 相似文献
977.
978.
James J. Gilroy Graham W. Prescott Johann S. Cardenas Pamela González del Pliego Castañeda Andrés Sánchez Luis E. Rojas‐Murcia Claudia A. Medina Uribe Torbjørn Haugaasen David P. Edwards 《Global Change Biology》2015,21(4):1531-1540
Oil palm agriculture is rapidly expanding in the Neotropics, at the expense of a range of natural and seminatural habitats. A key question is how this expansion should be managed to reduce negative impacts on biodiversity. Focusing on the Llanos of Colombia, a mixed grassland–forest system identified as a priority zone for future oil palm development, we survey communities of ants, dung beetles, birds and herpetofauna occurring in oil palm plantations and the other principal form of agriculture in the region – improved cattle pasture – together with those of surrounding natural forests. We show that oil palm plantations have similar or higher species richness across all four taxonomic groups than improved pasture. For dung beetles, species richness in oil palm was equal to that of forest, whereas the other three taxa had highest species richness in forests. Hierarchical modelling of species occupancy probabilities indicated that oil palm plantations supported a higher proportion of species characteristic of forests than did cattle pastures. Across the bird community, occupancy probabilities within oil palm were positively influenced by increasing forest cover in a surrounding 250 m radius, whereas surrounding forest cover did not strongly influence the occurrence of other taxonomic groups in oil palm. Overall, our results suggest that the conversion of existing improved pastures to oil palm has limited negative impacts on biodiversity. As such, existing cattle pastures of the Colombian Llanos could offer a key opportunity to meet governmental targets for oil palm development without incurring significant biodiversity costs. Our results also highlight the value of preserving remnant forests within these agricultural landscapes, protecting high biodiversity and exporting avian ‘spill‐over’ effects into oil palm plantations. 相似文献
979.
Development of a high-density cranberry SSR linkage map for comparative genetic analysis and trait detection 总被引:2,自引:0,他引:2
Brandon Schlautman Giovanny Covarrubias-Pazaran Luis A. Diaz-Garcia Jennifer Johnson-Cicalese Massimo Iorrizo Lorraine Rodriguez-Bonilla Tierney Bougie Tiffany Bougie Eric Wiesman Shawn Steffan James Polashock Nicholi Vorsa Juan Zalapa 《Molecular breeding : new strategies in plant improvement》2015,35(8):1-18
980.
José Alexandre Felizola Diniz Filho Fabricio Villalobos Luis Mauricio Bini 《Genetics and molecular biology》2015,38(3):396-400
Eigenfunction analyses have been widely used to model patterns of autocorrelation in time, space and phylogeny. In a phylogenetic context, Diniz-Filho et al. (1998) proposed what they called Phylogenetic Eigenvector Regression (PVR), in which pairwise phylogenetic distances among species are submitted to a Principal Coordinate Analysis, and eigenvectors are then used as explanatory variables in regression, correlation or ANOVAs. More recently, a new approach called Phylogenetic Eigenvector Mapping (PEM) was proposed, with the main advantage of explicitly incorporating a model-based warping in phylogenetic distance in which an Ornstein-Uhlenbeck (O-U) process is fitted to data before eigenvector extraction. Here we compared PVR and PEM in respect to estimated phylogenetic signal, correlated evolution under alternative evolutionary models and phylogenetic imputation, using simulated data. Despite similarity between the two approaches, PEM has a slightly higher prediction ability and is more general than the original PVR. Even so, in a conceptual sense, PEM may provide a technique in the best of both worlds, combining the flexibility of data-driven and empirical eigenfunction analyses and the sounding insights provided by evolutionary models well known in comparative analyses. 相似文献