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91.
92.
María de Lourdes Ramírez‐Ahuja Mayra A. Gmez‐Govea Angel Lugo‐Trampe Gissela Borrego‐Soto Ivn Delgado‐Enciso Gustavo Ponce‐Garcia Margarita L. Martínez‐Fierro Eda G. Ramírez‐Valles Víctor Trevio Adriana E. Flores‐Suarez Iram P. Rodríguez‐Sanchez 《Journal of Applied Entomology》2019,143(8):834-841
The microbiota of Hymenoptera is limited to certain bacterial species that vary according to the habitat and diet; however, the bacterial populations are not known in Telenomus tridentatus Johnson & Bin, an egg parasitoid of agricultural importance. We determined the microbiota composition of adults of T. tridentatus using next‐generation sequencing technologies. We found the presence of the Phylums Proteobacteria, in greater proportion followed by Actinobacteria and Firmicutes, respectively. The most abundant species were Cutibacterium acnes, Aquabacterium sp. and Massilia sp. The results of this study could lead to investigating the importance of symbiotic bacteria in T. tridentatus and its relation with agricultural plantations. 相似文献
93.
Victor G. Valcour Serena S. Spudich Napapon Sailasuta Nittaya Phanuphak Sukalaya Lerdlum James L. K. Fletcher Eugene D. M. B. Kroon Linda L. Jagodzinski Isabel E. Allen Collin L. Adams Peeriya Prueksakaew Bonnie M. Slike Joanna M. Hellmuth Jerome H. Kim Jintanat Ananworanich SEARCH /RV Study Group 《PloS one》2015,10(11)
Objective
To compare central nervous system (CNS) outcomes in participants treated during acute HIV infection with standard combination antiretroviral therapy (cART) vs. cART plus integrase inhibitor and CCR5 antagonist (cART+).Design
24-week randomized open-label prospective evaluation.Method
Participants were evaluated then randomized to initiate cART (efavirenz, tenofovir, and either emtricitabine or lamivudine) vs. cART+ (cART plus raltegravir and maraviroc) during acute HIV and re-evaluated at 4, 12 and 24 weeks. We examined plasma and CSF cytokines, HIV RNA levels, neurological and neuropsychological findings, and brain MRS across groups and compared to healthy controls.Results
At baseline, 62 participants were in Fiebig stages I-V. Randomized groups were similar for mean age (27 vs. 25, p = 0.137), gender (each 94% male), plasma log10 HIV RNA (5.4 vs. 5.6, p = 0.382), CSF log10 HIV RNA (2.35 vs. 3.31, p = 0.561), and estimated duration of HIV (18 vs. 17 days, p = 0.546). Randomized arms did not differ at 24 weeks by any CNS outcome. Combining arms, all measures concurrent with antiretroviral treatment improved, for example, neuropsychological testing (mean NPZ-4 of -0.408 vs. 0.245, p<0.001) and inflammatory markers by MRS (e.g. mean frontal white matter (FWM) choline of 2.92 vs. 2.84, p = 0.045) at baseline and week 24, respectively. Plasma neopterin (p<0.001) and interferon gamma-induced protein 10 (IP-10) (p = 0.007) remained elevated in participants compared to controls but no statistically significant differences were seen in CSF cytokines compared to controls, despite individual variability among the HIV-infected group.Conclusions
A 24-week course of cART+ improved CNS related outcomes, but was not associated with measurable differences compared to standard cART. 相似文献94.
Tropical forests are suffering from increasing intensities and frequency of disturbances. As a result, non-native species
accidentally introduced or intentionally planted for farming, plantations, and ornamental purposes may spread and potentially
invade undisturbed native forest. It is not known if these introduced species will become invasive, as a result of recurrent
natural disturbances such as hurricanes. Using data from three censuses (spanning 15 years) of a 16-ha subtropical wet forest
plot, we investigated the impact of two hurricanes on populations of plant species that were planted in farms and plantations
that were then abandoned and from the natural spread of species introduced into Puerto Rico in the past. The populations of
four species (Citrus paradis, Mangifera indica, Musa sp., and Simarouba glauca) changed little over time. Six species (Artocarpus altilis, Calophyllum calaba, Genipa americana, Hibiscus pernambucensis, Syzygium jambos, and Swietenia macrophylla) declined between the first two censuses after Hurricane Hugo, then increased again in Census 3 after Hurricane Georges.
Spathodea campanulata gradually increased from census to census, while Coffea arabica declined. These introduced species represent only a small part of the forest basal area and few show signs of increasing
over time. The number of stems per plant, new recruits, and the growth rates of these introduced species were within the ranges
of those for native plant species. The mortality rates over both census intervals were significantly lower for introduced
species (<5% year−1) than for native ones (15% year−1). Many new recruits established after Hurricane Hugo (prior to this study) had opened the forest canopy and these high mortality
rates reflect their death as the canopy recovered. Only Swietenia macrophylla and Syzygium jambos showed an increase in stem numbers in the closed canopy area of forest that had suffered limited human disturbance in the
past. A future increase in frequency of disturbance may enable greater stem numbers of introduced species to establish, while
lower-mortality rates compared to native species, may allow them to persist during inter-hurricane intervals. An increase
in the population of introduced species, especially for those that grow into large trees, may have an impact on this tropical
forest in the future. 相似文献
95.
Manutsanun Inthawong Piyanate Sunyakumthorn Sirima Wongwairot Tippawan Anantatat Susanna J. Dunachie Rawiwan Im-Erbsin James W. Jones Carl J. Mason Luis A. Lugo Stuart D. Blacksell Nicholas P. J. Day Piengchan Sonthayanon Allen L. Richards Daniel H. Paris 《PLoS neglected tropical diseases》2022,16(8)
BackgroundScrub typhus is a vector-borne febrile illness caused by Orientia tsutsugamushi transmitted by the bite of Trombiculid mites. O. tsutsugamushi has a high genetic diversity and is increasingly recognized to have a wider global distribution than previously assumed.Methodology/principle findingsWe evaluated the clinical outcomes and host immune responses of the two most relevant human pathogenic strains of O. tsutsugamushi; Karp (n = 4) and Gilliam (n = 4) in a time-course study over 80 days post infection (dpi) in a standardized scrub typhus non-human primate rhesus macaque model. We observed distinct features in clinical progression and immune response between the two strains; Gilliam-infected macaques developed more pronounced systemic infection characterized by an earlier onset of bacteremia, lymph node enlargement, eschar lesions and higher inflammatory markers during the acute phase of infection, when compared to the Karp strain. C-reactive protein (CRP) plasma levels, interferon gamma (IFN-γ, interleukin-1 receptor antagonist (IL-1ra), IL-15 serum concentrations, CRP/IL10- and IFN-γ/IL-10 ratios correlated positively with bacterial load in blood, implying activation of the innate immune response and preferential development of a T helper-type 1 immune response. The O. tsutsugamushi-specific immune memory responses in cells isolated from skin and lymph nodes at 80 dpi were more markedly elevated in the Gilliam-infected macaques than in the Karp-infected group. The comparative cytokine response dynamics of both strains revealed significant up-regulation of IFN-γ, tumor necrosis factor (TNF), IL-15, IL-6, IL-18, regulatory IL-1ra, IL-10, IL-8 and granulocyte-colony-stimulating factor (G-CSF). These data suggest that the clinical outcomes and host immune responses to scrub typhus could be associated with counter balancing effects of pro- and anti-inflammatory cytokine-mediated responses.Currently, no data on characterized time-course comparisons of O. tsutsugamushi strains regarding measures of disease severity and immune response is available. Our study provides evidence for the strain-specificity of host responses in scrub typhus, which supports our understanding of processes at the initial inoculation site (eschar), systemic disease progression, protective and/or pathogenic host immune mechanisms and cellular immune memory function.Conclusions/significanceThis study characterised an improved intradermal rhesus macaque challenge model for scrub typhus, whereby the Gilliam strain infection associated with higher disease severity in the rhesus macaque model than the previous Karp strain infection. Difficulties associated with inoculum quantitation for obligate-intracellular bacteria were overcome by using functional inoculum titrations in outbred mice. The Gilliam-based rhesus macaque model provides improved endpoint measurements and contributes towards the identification of correlates of protection for future vaccine development. 相似文献
96.
Audra F. Lugo 《Inorganica chimica acta》2010,363(10):2104-2511
A series of metal complexes have been prepared using two similar ketiminato ligands, ArL1H (([RN(H)(C(Me))2C(Me)O], ((R = 2,6-diisopropylphenyl (Dipp) or 2,6-dimethylphenyl, (Dmp)) and the less bulky, multidentate molecule L2H, ([RN(H)C(Me)CHC(Me)O] (R = C2H4NEt2)). Reaction of ArL1H or ArL1Na with Co(SCN)2, Eu(OTf)3 and Cu(OTf)2, afforded [Co(DippL1H)2(NCS)2] (1), [Eu(DippL1H)3(OTf)3] (2), and [Cu(OTf)2(DippL1H)2] (3). The coordination preferences of ArL1 and L2 with FeBr2 were investigated and yielded crystals of [Fe(DippL1)2] (4), [L2FeBr·LiBr(THF)2] (5), and [{(DmpNC(Me))2C(H)Me}FeBr2] (6). Complexes 1-6 have been structurally characterized by X-ray crystallography, and other pertinent techniques. 相似文献
97.
Heather D. Vance-Chalcraft Michael R. Willig Stephen B. Cox Ariel E. Lugo Frederick N. Scatena 《Biotropica》2010,42(3):290-299
Anthropogenic activities have accelerated the rate of global loss of biodiversity, making it more important than ever to understand the structure of biodiversity hotspots. One current focus is the relationship between species richness and aboveground biomass (AGB) in a variety of ecosystems. Nonetheless, species diversity, evenness, rarity, or dominance represent other critical attributes of biodiversity and may have associations with AGB that are markedly different than that of species richness. Using data from large trees in four environmentally similar sites in the Luquillo Experimental Forest of Puerto Rico, we determined the shape and strength of relationships between each of five measures of biodiversity (i.e., species richness, Simpson's diversity, Simpson's evenness, rarity, and dominance) and AGB. We quantified these measures of biodiversity using either proportional biomass or proportional abundance as weighting factors. Three of the four sites had a unimodal relationship between species richness and AGB, with only the most mature site evincing a positive, linear relationship. The differences between the mature site and the other sites, as well as the differences between our richness–AGB relationships and those found at other forest sites, highlight the crucial role that prior land use and severe storms have on this forest community. Although the shape and strength of relationships differed greatly among measures of biodiversity and among sites, the strongest relationships within each site were always those involving richness or evenness. 相似文献
98.
99.
We explore the consequences of modifying the way in which species are defined in an evolutionary food web model. In the original version of the model, the species were defined in terms of a fixed number of features, chosen from a large number of possibilities. These features represented phenotypic and behavioural characteristics of the species. Speciation consisted in occasionally replacing one of the features by another. Here we modify this scheme by firstly allowing for a richer structure and secondly by testing whether we are able to eliminate the need for an explicit choice of features altogether. In the first case we allow for changing the number of features which define a species, as well as their nature, and find that in the resulting webs the higher trophic levels typically contain species with the greatest number of features. In the second case, by a simplification of the mechanisms for inter and intra-species competition, we construct a model without any explicit features and find that we are still able to grow model food webs. We assess the quality of the food webs produced and discuss the consequences of our findings for the future modelling of food webs. 相似文献
100.
There are several studies that clearly indicate a close bidirectional communication between the neuroendocrine and immune systems. In this sense, hypothalamic releasing hormones, besides their neuroendocrine role, have been shown to influence immune functions. Despite studies developed in mammals, there is, as yet, no information available about the role of the pituitary adenylate cyclase-activating polypeptide (PACAP) and PACAP-related peptide (PRP) in the fish innate immune system. The present study has evaluated the effect of PACAP and PRP administered by bath immersion, on important parameters of innate immunity and antioxidant defenses in African catfish (Clarias gariepinus) fry. We have shown, for the first time, that administration of recombinant C. gariepinus PACAP not only promotes growth but also increases lysozyme, nitric oxide synthase-derived metabolites and antioxidant defenses in treated fry. From our results, PACAP appears to act as a regulator of the teleostean immune system, in addition to its physiological role in controlling growth of fish. 相似文献