Early social context does not influence behavioral variation at adulthood in ants

Early experience can prepare offspring to adapt their behaviors to the environment they are likely to encounter later in life. In several species of ants, colonies show ontogenic changes in the brood-to-worker ratio that are known to have an impact on worker morphology. However, little information is available on the influence of fluctuations in the early social context on the expression of behavior in adulthood. Using the ant Lasius niger, we tested whether the brood-to-worker ratio during larval stages influenced the level of behavioral variability at adult stages. We raised batches of 20 or 180 larvae in the presence of 60 workers until adulthood. We then quantified the activity level and wall-following tendency of callow workers on 10 successive trials to test the prediction that larvae reared under a high brood-to-worker ratio should show greater behavioral variations. We found that manipulation of the brood-to-worker ratio influenced the duration of development and the size of individuals at emergence. We detected no influence of early social context on the level of between- or within-individual variation measured for individual activity level or on wall-following behavior. Our study suggests that behavioral traits may be more canalized than morphological traits.     

CO2 methanation activated by magnetic heating: life cycle assessment and perspectives for successful renewable energy storage

Purpose

Technologies with low environmental impacts and promoting renewable energy sources are required to meet the energetic demand while facing the increase of gas emissions associated to the greenhouse effect and the depletion of fossil fuels. CO₂ methanation activated by magnetic heating has recently been reported as a highly efficient and innovative power-to-gas technology in a perspective of successful renewable energy storage and carbon dioxide valorisation. In this work, the life cycle assessment (LCA) of this process is performed, in order to highlight the environmental potential of the technology, and its competitivity with in respect to conventional heating technologies.

Methods

The IMPACT 2002+ was used for this LCA. The process studied integrates methanation, water electrolysis and CO₂ capture and separation. This “cradle-to-gate” LCA study does not consider the use of methane, which is the reaction product. The functional unit used is the energy content of the produced CH₄. The LCA was carried out using the energy mix data for the years 2020 and 2050 as given by the French Agency for Environment and Energy management (ADEME). Consumption data were either collected from literature or obtained from the LPCNO measurements as discussed by Marbaix (2019). The environmental impact of the CO₂ methanation activated by magnetic heating was compared with the environmental impact of a power-to-gas plant using conventional heating (Helmeth) and considering the environmental impact of the natural gas extraction.

Results

It is shown that the total flow rate of reactants, the source of CO₂ and the energy mix play a major role on the environmental impact of sustainable CH₄ production, whereas the lifetime of the considered catalyst has no significant influence. As a result of the possible improvements on the above-mentioned parameters, the whole process is expected to reduce by 75% in its environmental impact toward 2050. This illustrates the high environmental potential of the methanation activated by magnetic heating when coupled with industrial exhausts and renewable electricity production.

Conclusions

The technology is expected to be environmentally competitive compared with existing similar processes using external heating sources with the additional interest of being extremely dynamic in response, in line with the intermittency of renewable energy production.

     

Mapping Topoisomerase IV Binding and Activity Sites on the E. coli Genome

Catenation links between sister chromatids are formed progressively during DNA replication and are involved in the establishment of sister chromatid cohesion. Topo IV is a bacterial type II topoisomerase involved in the removal of catenation links both behind replication forks and after replication during the final separation of sister chromosomes. We have investigated the global DNA-binding and catalytic activity of Topo IV in E. coli using genomic and molecular biology approaches. ChIP-seq revealed that Topo IV interaction with the E. coli chromosome is controlled by DNA replication. During replication, Topo IV has access to most of the genome but only selects a few hundred specific sites for its activity. Local chromatin and gene expression context influence site selection. Moreover strong DNA-binding and catalytic activities are found at the chromosome dimer resolution site, dif, located opposite the origin of replication. We reveal a physical and functional interaction between Topo IV and the XerCD recombinases acting at the dif site. This interaction is modulated by MatP, a protein involved in the organization of the Ter macrodomain. These results show that Topo IV, XerCD/dif and MatP are part of a network dedicated to the final step of chromosome management during the cell cycle.     

Nα-terminal acetylation of proteins by NatA and NatB serves distinct physiological roles in Saccharomyces cerevisiae

1. Download : Download high-res image (176KB)
2. Download : Download full-size image