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排序方式: 共有441条查询结果,搜索用时 953 毫秒
141.
Stefan Bayer Ludger Keilig Dominik Kraus Manfred Grüner Helmut Stark Sebastian Mues Norbert Enkling 《Gerodontology》2011,28(3):221-226
Gerodontology 2010; doi: 10.1111/j.1741‐2358.2009.00352.x Influence of the lubricant and the alloy on the wear behaviour of attachments Objectives: Wear of attachments leads to a loss of retention and reduces the function of overdentures. This study evaluated the retention force changes of an attachment system for overdentures. The influence of the lubricant and the alloy on wear constancy was examined. Methods: Cylindrical anchors of the Dalbo®‐Z system were tested (Cendres+Métaux SA). Three groups of alloy‐lubricant combinations were generated 1.Elitor®/NaCl‐solution (EN) 2.Elitor®/Glandosane®aquadest. (EG) and 3.Valor®/Glandosane®/aquadest. (VG). Ten samples of each group were subjected to 10 000 insertion–separation cycles. Results: For the EN‐group, this led to a large increase in retention force. The EG‐ and VG‐group showed a constant decrease after an initial increase in retention force at the beginning of the wear simulation. The change of the alloy caused no statistically significant differences. The use of a more viscous lubricant reduced the retention force increase significantly. Conclusions: The use of a lubricant which simulates clinical conditions is an absolute need for wear simulation because the retention force changes are influenced enormously. The change of the alloy at the Dalbo®‐Z system did not influence the wear behaviour. As a slight decrease in retention force was recorded, it is useful for an attachment system to allow compensation with an adjustable matrix. 相似文献
142.
Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases 总被引:6,自引:0,他引:6
Grimmler M Wang Y Mund T Cilensek Z Keidel EM Waddell MB Jäkel H Kullmann M Kriwacki RW Hengst L 《Cell》2007,128(2):269-280
p27Kip1 controls cell proliferation by binding to and regulating the activity of cyclin-dependent kinases (Cdks). Here we show that Cdk inhibition and p27 stability are regulated through direct phosphorylation by tyrosine kinases. A conserved tyrosine residue (Y88) in the Cdk-binding domain of p27 can be phosphorylated by the Src-family kinase Lyn and the oncogene product BCR-ABL. Y88 phosphorylation does not prevent p27 binding to cyclin A/Cdk2. Instead, it causes phosphorylated Y88 and the entire inhibitory 3(10)-helix of p27 to be ejected from the Cdk2 active site, thus restoring partial Cdk activity. Importantly, this allows Y88-phosphorylated p27 to be efficiently phosphorylated on threonine 187 by Cdk2 which in turn promotes its SCF-Skp2-dependent degradation. This direct link between transforming tyrosine kinases and p27 may provide an explanation for Cdk kinase activities observed in p27 complexes and for premature p27 elimination in cells that have been transformed by activated tyrosine kinases. 相似文献
143.
Salceda E Pérez-Castells J López-Méndez B Garateix A Salazar H López O Aneiros A Ständker L Béress L Forssmann WG Soto E Jiménez-Barbero J Giménez-Gallego G 《The Biochemical journal》2007,406(1):67-76
CgNa (Condylactis gigantea neurotoxin) is a 47-amino-acid- residue toxin from the giant Caribbean sea anemone Condylactis gigantea. The structure of CgNa, which was solved by 1H-NMR spectroscopy, is somewhat atypical and displays significant homology with both type I and II anemone toxins. CgNa also displays a considerable number of exceptions to the canonical structural elements that are thought to be essential for the activity of this group of toxins. Furthermore, unique residues in CgNa define a characteristic structure with strong negatively charged surface patches. These patches disrupt a surface-exposed cluster of hydrophobic residues present in all anemone-derived toxins described to date. A thorough characterization by patch-clamp analysis using rat DRG (dorsal root ganglion) neurons indicated that CgNa preferentially binds to TTX-S (tetrodotoxin-sensitive) voltage-gated sodium channels in the resting state. This association increased the inactivation time constant and the rate of recovery from inactivation, inducing a significant shift in the steady state of inactivation curve to the left. The specific structural features of CgNa may explain its weaker inhibitory capacity when compared with the other type I and II anemone toxins. 相似文献
144.
Summary. So-called energy drinks with very high amounts of taurine (up to 4000 mg/l are usually granted by certificates of exemption)
are increasingly offered on the market. To control the currently valid maximum limits of taurine in energy drinks, a simple
and rapid analytical method is required to use it routinely in food monitoring. In this article, we describe a fast and efficient
analytical method (FTIR-spectroscopy) that is able to reliably characterize and quantify taurine in energy drinks. The determination
of taurine in energy drinks by FTIR was compared with amino acid analyzer (ion chromatography with ninhydrin-postcolumn derivatization).
During analysis of 80 energy drinks, a median concentration of 3180 mg/l was found in alcohol-free products, 314 mg/l in energy
drinks with spirits, 151 mg/l in beer-containing drinks and 305 mg/l in beverages with wine. Risk analysis of these products
is difficult due to the lack of valid toxicological information about taurine and its interferences with other ingredients
of energy drinks (for example caffeine and alcohol). So far, the high taurine concentrations of energy drinks in comparison
to the rest of the diet are scientifically doubtful, as the advertised physiological effects and the value of supplemented
taurine are unproven. 相似文献
145.
Shiga toxin B-subunit (STxB), a protein involved in the cell-binding and intracellular trafficking of Shiga holotoxin, binds to a specific glycolipid, the globotriaosyl ceramide (Gb(3)). Tryptophan residues of STxB, located at the protein-membrane interface, allow one to study its interaction with model membranes by means of spectroscopic methods with no need for chemical derivatisation with a fluorophore. The protein emits maximally around 346 nm and a blue shift of about 8 nm, as well as the occurrence of changes in the emission fluorescence intensity spectra, is indicative of insertion and partition into the membrane. However, the interaction seems to take place without pentamer dissociation. Acrylamide quenching experiments confirm tryptophan residues become less exposed to solvent when in the presence of vesicles, and the use of lipophilic probes suggests that they are located in a shallow position near the water/membrane interface. Fluorescence intensity and lifetime measurements upon STxB titration with Gb(3)-containing vesicles suggest a complex STxB/Gb(3) docking mechanism involving static quenching in the later stages. Based on our observations, a model of the protein-membrane interaction is proposed and the STxB membrane partition and binding constants were calculated. 相似文献
146.
Vornam Barbara Leinemann Ludger Peters Franziska S. Wolff Alexander Leha Andreas Salinas Gabriela Schumacher Joerg Gailing Oliver 《Plant Molecular Biology Reporter》2019,37(3):214-223
Plant Molecular Biology Reporter - The ascomycete Sphaeropsis sapinea (syn. Diplodia pinea), the causal agent of Diplodia blight of pine, is also known to be an endophyte with a latent pathogenic... 相似文献
147.
148.
Zheng Lei Evlyukhin Andrey Overmeyer Ludger Reinhardt Carsten 《Plasmonics (Norwell, Mass.)》2019,14(6):1547-1554
Plasmonics - 3D metallic structures with symmetrically curved surfaces are proposed for surface plasmon polaritons (SPPs) deflection and concentration. Two-photon polymerization (2PP) and a... 相似文献
149.
Astrid Liedert Viktoria R?ntgen Thorsten Schinke Peggy Benisch Regina Ebert Franz Jakob Ludger Klein-Hitpass Jochen K. Lennerz Michael Amling Anita Ignatius 《PloS one》2014,9(7)
The canonical Wnt/β-catenin pathway plays a key role in the regulation of bone remodeling in mice and humans. Two transmembrane proteins that are involved in decreasing the activity of this pathway by binding to extracellular antagonists, such as Dickkopf 1 (Dkk1), are the low-density lipoprotein receptor related protein 5 (Lrp5) and Kremen 2 (Krm2). Lrp 5 deficiency (Lrp5−/−) as well as osteoblast-specific overexpression of Krm2 in mice (Col1a1-Krm2) result in severe osteoporosis occurring at young age. In this study, we analyzed the influence of Lrp5 deficiency and osteoblast-specific overexpression of Krm2 on fracture healing in mice using flexible and semi-rigid fracture fixation. We demonstrated that fracture healing was highly impaired in both mouse genotypes, but that impairment was more severe in Col1a1-Krm2 than in Lrp5−/− mice and particularly evident in mice in which the more flexible fixation was used. Bone formation was more reduced in Col1a1-Krm2 than in Lrp5−/− mice, whereas osteoclast number was similarly increased in both genotypes in comparison with wild-type mice. Using microarray analysis we identified reduced expression of genes mainly involved in osteogenesis that seemed to be responsible for the observed stronger impairment of healing in Col1a1-Krm2 mice. In line with these findings, we detected decreased expression of sphingomyelin phosphodiesterase 3 (Smpd3) and less active β-catenin in the calli of Col1a1-Krm2 mice. Since Krm2 seems to play a significant role in regulating bone formation during fracture healing, antagonizing KRM2 might be a therapeutic option to improve fracture healing under compromised conditions, such as osteoporosis. 相似文献
150.
Etmar Bulk Jun Yu Antje Hascher Steffen Koschmieder Rainer Wiewrodt Utz Krug Bernd Timmermann Alessandro Marra Ludger Hillejan Karsten Wiebe Wolfgang E. Berdel Albrecht Schwab Carsten M��ller-Tidow 《PloS one》2012,7(12)
Alterations of Eph receptor tyrosine kinases are frequent events in human cancers. Genetic variations of EPHB6 have been described but the functional outcome of these alterations is unknown. The current study was conducted to screen for the occurrence and to identify functional consequences of EPHB6 mutations in non-small cell lung cancer. Here, we sequenced the entire coding region of EPHB6 in 80 non-small cell lung cancer patients and 3 tumor cell lines. Three potentially relevant mutations were identified in primary patient samples of NSCLC patients (3.8%). Two point mutations led to instable proteins. An in frame deletion mutation (del915-917) showed enhanced migration and accelerated wound healing in vitro. Furthermore, the del915-917 mutation increased the metastatic capability of NSCLC cells in an in vivo mouse model. Our results suggest that EPHB6 mutations promote metastasis in a subset of patients with non-small cell lung cancer. 相似文献