Comparison of two methods for recovery of ovarian oocytes from slaughter cattle

When aspiration was used, 80 oocytes were recovered from 185 ovarian follicles, whereas 155 oocytes were recovered by rupturing 154 isolated follicles (P < 0.01).Two oocytes were found in each of three isolated follicles, three oocytes in one follicle and no oocytes in four follicles.Of the oocytes recovered by aspiration, 45% were morphologically normal, whereas 63.2% morphologically normal oocytes were recovered from isolated follicles (P < 0.01).