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51.
Corrêa Daniele Bussioli Alves do Amaral Danilo Trabuco da Silva Márcio José Destéfano Suzete Aparecida Lanza 《Antonie van Leeuwenhoek》2021,114(7):913-931
Antonie van Leeuwenhoek - This study aimed to characterize six Streptomyces strains associated with potato scab in south Brazil through polyphasic taxonomy involving morphology, pathogenicity and... 相似文献
52.
In southern Brazil, we investigated shark and ray capture records in an integral protection marine protection area (MPA) that allows fishing in that area. We found 10 shark and nine ray species, of which 88.5% and 66.2%, respectively, are endangered. Female adults and neonates of both sexes are abundant in the area, with records of stranding of large specimens, postcapture abortions and ovigerous capsules with fresh embryos on the beach. We suggest the application of continuous fisheries monitoring and an increase in guidelines on the capture of endangered species. 相似文献
53.
Volpi Denise Alves Fabiana Villa da Silva Arguelho Alan do Vale Marcos Martinez Deniz Matheus Zopollatto Maity 《International journal of biometeorology》2021,65(10):1695-1705
International Journal of Biometeorology - The aim of this study was to estimate, using data mining, which microclimate and behavioral variables affect the behavior of animals to seek shaded or... 相似文献
54.
Petsch Danielle Katharine Ribas Luiz Guilherme dos Santos Mantovano Tatiane Pulzatto Mikaela Marques Alves Andreia Teixeira Pinha Gisele Daiane Thomaz Sidinei Magela 《Hydrobiologia》2021,848(9):2319-2330
Hydrobiologia - Biological invasions and climate change are important drivers of biodiversity loss. In freshwater ecosystems, golden and zebra mussels are two highly aggressive invasive species... 相似文献
55.
56.
Joost Groot Yizhou Zhou Eric Marshall Patrick Cullen Thomas Carlile Dongdong Lin Chong-Feng Xu Justin Crisafulli Chao Sun Fergal Casey Baohong Zhang Christina Alves 《Biotechnology journal》2021,16(8):2000548
In recent years, High-Throughput Sequencing (HTS) based methods to detect mutations in biotherapeutic transgene products have become a key quality step deployed during the development of manufacturing cell line clones. Previously we reported on a higher throughput, rapid mutation detection method based on amplicon sequencing (targeting transgene RNA) and detailed its implementation to facilitate cell line clone selection. By gaining experience with our assay in a diverse set of cell line development programs, we improved the computational analysis as well as experimental protocols. Here we report on these improvements as well as on a comprehensive benchmarking of our assay. We evaluated assay performance by mixing amplicon samples of a verified mutated antibody clone with a non-mutated antibody clone to generate spike-in mutations from ∼60% down to ∼0.3% frequencies. We subsequently tested the effect of 16 different sample and HTS library preparation protocols on the assay's ability to quantify mutations and on the occurrence of false-positive background error mutations (artifacts). Our evaluation confirmed assay robustness, established a high confidence limit of detection of ∼0.6%, and identified protocols that reduce error levels thereby significantly reducing a source of false positives that bottlenecked the identification of low-level true mutations. 相似文献
57.
Liliane Bezerra de Lima Wliana Alves Viturino da Silva Ewelyn Cintya Felipe dos Santos Janaína Carla Barbosa Machado Thamara Figueiredo Procópio Maiara Celine de Moura Thiago Henrique Napoleão Magda Rhayanny Assunção Ferreira Luiz Alberto Lira Soares 《化学与生物多样性》2021,18(12):e2100538
The aim of this study was to evaluate the phytochemical composition, antioxidant, and antimicrobial potential of crude extract and fractions of Punica granatum leaves. The extract was produced by turbo extraction, after which hexanic, ethyl acetate, and aqueous fractions were obtained by partitioning. The chemical analyses were performed by thin layer chromatography and high-performance liquid chromatography, and the antioxidant activities were assayed by DPPH. and ABTS.+. Minimal inhibitory and bactericidal concentrations (MIC/MBC) were applied to twenty-two bacteria. Most strains susceptible to extract/fractions and resistant to antibiotics were selected, and ampicillin, azithromycin, ciprofloxacin, and gentamicin were associated with the ethyl acetate fraction (EAF) against multidrug-resistant strains in modulatory and checkboard models. The data from chromatographic analyses showed flavonoids and tannins in the extract, as well as the enrichment of EAF in phenols, mainly flavonoids. The flavonoids were connected to the electron transfer activity demonstrated in the DPPH. and ABTS.+ assays. Gram-positive strains are more susceptible to EAF. The subinhibitory concentrations of P. granatum enhanced the antimicrobial activity of the agents and reduced the EAF individual MIC, and the combination of EAF and antibiotics demonstrated a synergistic effect. These results present a promising approach for developing a therapy in which antioxidant extracts and fractions can be used in combination with antibiotics. 相似文献
58.
Lara Soares Aleixo de Carvalho Lívia Mara Silva Vinícius Carius de Souza Marcos Paulo Nascimento da Silva Priscila V. S. Z. Capriles Priscila de Faria Pinto Josué de Moraes Ademar Alves Da Silva Filho 《化学与生物多样性》2021,18(11):e2100604
Schistosomiasis, a neglected tropical disease caused by Schistosoma species, harms over 250 million people in several countries. The treatment is achieved with only one drug, praziquantel. Cardamonin, a natural chalcone with in vitro schistosomicidal activity, has not been in vivo evaluated against Schistosoma. In this work, we evaluated the in vivo schistosomicidal activities of cardamonin against Schistosoma mansoni worms and conducted enzymatic apyrase inhibition assay, as well as molecular docking analysis of cardamonin against potato apyrase, S. mansoni NTPDase 1 and S. mansoni NTPDase 2. In a mouse model of schistosomiasis, the oral treatment with cardamonin (400 mg/kg) showed efficacy against S. mansoni, decreasing the total worm load in 46.8 % and reducing in 54.5 % the number of eggs in mice. Cardamonin achieved a significant inhibition of the apyrase activity and the three-dimensional structure of the potato apyrase, obtained by homology modeling, showed that cardamonin may interact mainly through hydrogen bonds. Molecular docking studies corroborate with the action of cardamonin in binding and inhibiting both potato apyrase and S. mansoni NTPDases. 相似文献
59.
Daniel Silva Torres Bruna Alves de Oliveira Lígia Souza d Silveira Marcos Paulo da Silva Vinícius Rodrigues Durães Pereira Josué Moraes Mara Rúbia Costa Couri Rafaella Fortini Grenfell e Queiroz Patrícia Martins Parreiras Márcio Roberto Silva Lara Azevedo Alves Vinícius Carius de Souza Priscila Vanessa Zabala Capriles Goliatt Eveline Gomes Vasconcelos Ademar Alves da Silva Filho Priscila de Faria Pinto 《化学与生物多样性》2021,18(11):e2100439
In this work, two synthetic aurones revealed moderate schistosomicidal potential in in vitro and in vivo assays. Aurones ( 1 ) and ( 2 ) promoted changes in tegument integrity and motor activity, leading to death of adult Schistosoma mansoni worms in in vitro assays. When administered orally (two doses of 50 mg/kg) in experimentally infected animals, synthetic aurones ( 1 ) and ( 2 ) promoted reductions of 56.20 % and 57.61 % of the parasite load and stimulated the displacement towards the liver of the remaining adult worms. The oogram analysis revealed that the treatment with both aurones interferes with the egg development kinetics in the intestinal tissue. Seeking an action target for compounds ( 1 ) and ( 2 ), the connection with NTPDases enzymes, recognized as important therapeutic targets for S. mansoni, was evaluated. Molecular docking studies have shown promising results. The dataset reveals the anthelmintic character of these compounds, which can be used in the development of new therapies for schistosomiasis. 相似文献
60.
Henrique Barbosa Thais A. Costa-Silva Geanne A. Alves Conserva Adelson J. Araujo Ana Luísa L. Lordello Guilherme M. Antar Maiara Amaral Marisi G. Soares Andre G. Tempone João Henrique G. Lago 《化学与生物多样性》2021,18(4):e2001022
One new aporphine, dicentrine-β-N-oxide ( 1 ), together with five related known alkaloids dehydrodicentrine ( 2 ), predicentrine ( 3 ), N-methyllaurotetanine ( 4 ), cassythicine ( 5 ), and dicentrine ( 6 ) were isolated from the leaves of Ocotea puberula (Lauraceae). Antiprotozoal activity of the isolated compounds was evaluated in vitro against trypomastigote forms of Trypanosoma cruzi. Among the tested compounds, alkaloid 1 exhibited higher potential with EC50 value of 18.2 μM and reduced toxicity against NCTC cells (CC50>200 μM – SI>11.0), similar to positive control benznidazole (EC50 of 17.7 μM and SI=10.7). Considering the promising results of dicentrine-β-N-oxide ( 1 ) against trypomastigotes, the mechanism of parasite death caused by this alkaloid was investigated. As observed, this compound reached the plasma membrane electric potential directly after 2 h of incubation and triggered mitochondrial depolarization, which probably leads to trypomastigote death. Therefore, dicentrine-β-N-oxide ( 1 ), reported for the first time in this work, can contribute to future works for the development of new trypanocidal agents. 相似文献