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Talukdar Muhammad Waliullah Akter MST Yeasmin Atsuki Kaneko Naoki Koike Mashu Terasawa Takaya Totsuka 《Bioscience, biotechnology, and biochemistry》2017,81(2):307-310
Autophagic degradation of ribosomes is promoted by nutrient starvation and inactivation of target of rapamycin complex 1 (TORC1). Here we show that selective autophagic degradation of ribosomes (called ribophagy) after TORC1 inactivation requires the specific autophagy receptor Atg11. Rim15 protein kinase upregulated ribophagy, while it downregulated non-selective degradation of ribosomes. 相似文献
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A mannose/glucose specific lectin have been isolated and purified from mulberry seeds by affinity chromatography on ConA Sepharose.
The lectin is monomer in nature as judged by SDS-PAGE and its MW was estimated to be 22,000. The lectin is glycoprotein with
neutral sugar content of 28.57%, and mannose and glucose were identified as carbohydrate. The lectin agglutinated rat red
blood cells and in a hapten inhibition test, D-mannose and D-glucose was found to be inhibitor. The lectin also exhibited cytotoxic effect in brine shrimp lethality bioassay. The N-terminal
sequences of the lectin upto 45-residues except the positions of 21, 39, 42 and 44 were identified. Sequence homology of the
lectin is also discussed. 相似文献
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Yoshihito Shimizu Masayoshi Nagai Akter MST Yeasmin Naoki Koike Muhammad Waliullah Talukdar 《Bioscience, biotechnology, and biochemistry》2016,80(3):473-478
The mitotic separase cleaves Scc1 in cohesin to allow sister chromatids to separate from each other upon anaphase onset. Separase is also required for DNA damage repair. Here, we isolated and characterized 10 temperature-sensitive (ts) mutants of separase ESP1 in the budding yeast Saccharomyces cerevisiae. All mutants were defective in sister chromatid separation at the restricted temperature. Some esp1-ts mutants were hypersensitive to the microtubule poison benomyl and/or the DNA-damaging agent bleomycin. Overexpression of securin alleviated the growth defect in some esp1-ts mutants, whereas it rather exacerbated it in others. The Drosophila Pumilio homolog MPT5 was isolated as a high-dosage suppressor of esp1-ts cells. We discuss various features of separase based on these findings. 相似文献
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Nadia Caccamo Giuliana Guggino Serena Meraviglia Giuseppe Gelsomino Paola Di Carlo Lucina Titone Marialuisa Bocchino Domenico Galati Alessandro Matarese Jan Nouta Michel R. Klein Alfredo Salerno Alessandro Sanduzzi Francesco Dieli Tom H. M. Ottenhoff 《PloS one》2009,4(5)
CD8 T-cells contribute to control of Mycobacterium tuberculosis infection, but little is known about the quality of the CD8 T-cell response in subjects with latent infection and in patients with active tuberculosis disease. CD8 T-cells recognizing epitopes from 6 different proteins of Mycobacterium tuberculosis were detected by tetramer staining. Intracellular cytokines staining for specific production of IFN-γ and IL-2 was performed, complemented by phenotyping of memory markers on antigen-specific CD8 T-cells. The ex-vivo frequencies of tetramer-specific CD8 T-cells in tuberculous patients before therapy were lower than in subjects with latent infection, but increased at four months after therapy to comparable percentages detected in subjects with latent infection. The majority of CD8 T-cells from subjects with latent infection expressed a terminally-differentiated phenotype (CD45RA+CCR7−). In contrast, tuberculous patients had only 35% of antigen-specific CD8 T-cells expressing this phenotype, while containing higher proportions of cells with an effector memory- and a central memory-like phenotype, and which did not change significantly after therapy. CD8 T-cells from subjects with latent infection showed a codominance of IL-2+/IFN-γ+ and IL-2−/IFN-γ+ T-cell populations; interestingly, only the IL-2+/IFN-γ+ population was reduced or absent in tuberculous patients, highly suggestive of a restricted functional profile of Mycobacterium tuberculosis-specific CD8 T-cells during active disease. These results suggest distinct Mycobacterium tuberculosis specific CD8 T-cell phenotypic and functional signatures between subjects which control infection (subjects with latent infection) and those who do not (patients with active disease). 相似文献
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Archer NP Langlois PH Suarez L Brender J Shanmugam R 《Birth defects research. Part A, Clinical and molecular teratology》2007,79(1):27-34
BACKGROUND: Unlike maternal age, the effect of paternal age on birth defect prevalence has not been well examined. We used cases from the Texas birth defect registry, born during 1996-2002, to evaluate the association of paternal age with the prevalence of selected structural birth defects. METHODS: Poisson regression was used to calculate prevalence ratios (PRs) and 95% confidence intervals (CIs) associated with paternal age for each birth defect, adjusting for maternal age, race/ethnicity, and parity. RESULTS: Relative to fathers ages 25-29 years, fathers 20-24 years of age were more likely to have offspring with gastroschisis (PR 1.47, 95% CI: 1.12-1.94), and fathers 40+ years old were less likely to have offspring with trisomy 13 (PR 0.40, 95% CI: 0.16-0.96). No association was seen between paternal age and prevalence of anencephaly and encephalocele. A selection bias was observed for the other birth defects in which cases of younger fathers were more often excluded from study. CONCLUSIONS: In studies of birth defect risk and paternal age, the source of information may affect the validity of findings. 相似文献
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A CO2-Flux Mechanism Operating via pH-Polarity in Hydrilla Verticillata Leaves With C3 and C4 Photosynthesis 总被引:1,自引:0,他引:1
The aquatic angiosperm Hydrilla verticillata lacks Kranz anatomy, but has an inducible, C4-based, CO2 concentrating mechanism (CCM) that concentrates CO2 in the chloroplasts. Both C3 and C4
Hydrilla leaves showed light-dependent pH polarity that was suppressed by high dissolved inorganic carbon (DIC). At low DIC (0.25
mol m−3), pH values in the unstirred water layer on the abaxial and adaxial sides of the leaf were 4.2 and10.3, respectively. Abaxial
apoplastic acidification served as a CO2 flux mechanism (CFM), making HCO3
− available for photosynthesis by conversion to CO2. DIC at 10 mol m−3 completely suppressed acidification and alkalization. The data, along with previous results, indicated that inhibition was
specific to DIC, and not a buffer effect. Acidification and alkalization did not necessarily show 1:1 stoichiometry; their
kinetics for the apolar induction phase differed, and alkalization was less inhibited by 2.5 mol m−3 DIC. At low irradiance (50 μmol photons m−2 s−1), where CCM activity in C4 leaves is minimized, both leaf types had similar DIC inhibition of pH polarity. However, as irradiance increased, DIC inhibition
of C3 leaves decreased. In C4 leaves the CFM and CCM seemed to compete for photosynthetic ATP and/or reducing power. The CFM may require less, as at low
irradiance it still operated maximally, if [DIC] was low. Iodoacetamide (IA), which inhibits CO2 fixation in Hydrilla, also suppressed acidification and alkalization, especially in C4 leaves. IA does not inhibit the C4 CCM, which suggests that the CFM and CCM can operate independently. It has been hypothesized that irradiance and DIC regulate
pH polarity by altering the chloroplastic [DIC], which effects the chloroplast redox state and subsequently redox regulation
of a plasma-membrane H+-ATPase. The results lend partial support to a down-regulatory role for high chloroplastic [DIC], but do not exclude other
sites of DIC action. IA inhibition of pH polarity seems inconsistent with the chloroplast NADPH/NADP+ ratio being the redox transducer. The possibility that malate and oxaloacetate shuttling plays a role in CFM regulation requires
further investigation.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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