排序方式: 共有47条查询结果,搜索用时 15 毫秒
41.
Annika Wahl Silva Kasela Elena Carnero-Montoro Maarten van Iterson Jerko Štambuk Sapna Sharma Erik van den Akker Lucija Klaric Elisa Benedetti Genadij Razdorov Irena Trbojević-Akmačić Frano Vučković Ivo Ugrina Marian Beekman Joris Deelen Diana van Heemst Bastiaan T. Heijmans Christian Gieger 《Biochimica et Biophysica Acta (BBA)/General Subjects》2018,1862(3):637-648
Background
Glycosylation is one of the most common post-translation modifications with large influences on protein structure and function. The effector function of immunoglobulin G (IgG) alters between pro- and anti-inflammatory, based on its glycosylation. IgG glycan synthesis is highly complex and dynamic.Methods
With the use of two different analytical methods for assessing IgG glycosylation, we aim to elucidate the link between DNA methylation and glycosylation of IgG by means of epigenome-wide association studies. In total, 3000 individuals from 4 cohorts were analyzed.Results
The overlap of the results from the two glycan measurement panels yielded DNA methylation of 7 CpG-sites on 5 genomic locations to be associated with IgG glycosylation: cg25189904 (chr.1, GNG12); cg05951221, cg21566642 and cg01940273 (chr.2, ALPPL2); cg05575921 (chr.5, AHRR); cg06126421 (6p21.33); and cg03636183 (chr.19, F2RL3). Mediation analyses with respect to smoking revealed that the effect of smoking on IgG glycosylation may be at least partially mediated via DNA methylation levels at these 7 CpG-sites.Conclusion
Our results suggest the presence of an indirect link between DNA methylation and IgG glycosylation that may in part capture environmental exposures.General significance
An epigenome-wide analysis conducted in four population-based cohorts revealed an association between DNA methylation and IgG glycosylation patterns. Presumably, DNA methylation mediates the effect of smoking on IgG glycosylation. 相似文献42.
Ivan Radman Barbara Wessner Norbert Bachl Lana Ruzic Markus Hackl Arnold Baca Goran Markovic 《PloS one》2016,11(1)
The study aimed to evaluate the test–retest reliability of a newly developed 356 Soccer Shooting Test (356-SST), and the discriminative ability of this test with respect to the soccer players'' proficiency level and leg dominance. Sixty-six male soccer players, divided into three groups based on their proficiency level (amateur, n = 24; novice semi-professional, n = 18; and experienced semi-professional players, n = 24), performed 10 kicks following a two-step run up. Forty-eight of them repeated the test on a separate day. The following shooting variables were derived: ball velocity (BV; measured via radar gun), shooting accuracy (SA; average distance from the ball-entry point to the goal centre), and shooting quality (SQ; shooting accuracy divided by the time elapsed from hitting the ball to the point of entry). No systematic bias was evident in the selected shooting variables (SA: 1.98±0.65 vs. 2.00±0.63 m; BV: 24.6±2.3 vs. 24.5±1.9 m s-1; SQ: 2.92±1.0 vs. 2.93±1.0 m s-1; all p>0.05). The intra-class correlation coefficients were high (ICC = 0.70–0.88), and the coefficients of variation were low (CV = 5.3–5.4%). Finally, all three 356-SST variables identify, with adequate sensitivity, differences in soccer shooting ability with respect to the players'' proficiency and leg dominance. The results suggest that the 356-SST is a reliable and sensitive test of specific shooting ability in men’s soccer. Future studies should test the validity of these findings in a fatigued state, as well as in other populations. 相似文献
43.
Sporis G Ruzic L Leko G 《Journal of strength and conditioning research / National Strength & Conditioning Association》2008,22(2):559-566
The purpose of this study was to evaluate changes in anaerobic endurance in elite First-league soccer players throughout 2 consecutive seasons, in 2 phases, with and without high-intensity situational drills. Eighteen soccer players were tested before and after the 8-week summer conditioning and again in the next season. The measured variables included 300-yard shuttle run test, maximal heart rate, and maximal blood lactate at the end of the test. During the first phase of the study, the traditional sprint training was performed only 2 x weeks and consisted of 15 bouts of straight-line sprinting. In the second year the 4 x 4 min drills at an intensity of 90-95% of HRmax, separated by periods of 3-minute technical drills at 55-65% of HRmax were introduced. Statistical significance was set at P = 0.05. The traditional conditioning program conducted during the first year of the study did not elicit an improvement in anaerobic endurance as recorded in the 300-yard shuttle run test. After the intervention, the overall test running time improved significantly (55.74 +/- 1.63 s vs. 56.99 +/- 1.64 s; P < 0.05) with the maximal blood lactate at the end of the test significantly greater (15.4 +/- 1.23 mmol.L vs. 13.5 +/- 1.12 mmol.L. P < 0.01). As a result, this study showed some indication that situational high-intensity task training was more efficient than straight-line sprinting in improving anaerobic endurance measured by the 300-yard shuttle run test. 相似文献
44.
Ivana Buj Lucija Raguž Zoran Marčić Marko Ćaleta Aljoša Duplić Davor Zanella Perica Mustafić Lucija Ivić Sven Horvatić Roman Karlović 《Zeitschrift fur angewandte Ichthyologie》2021,37(1):20-37
National park Plitvice Lakes is the oldest, the largest and the most visited national park in Croatia, well known for its stunning karstic watersheds that supply Plitvice Lakes and exceptionally valuable biodiversity. Lack of knowledge on the taxonomic status, population structure and viability of trout in the Plitvice Lakes National park, as well as the possibility of existence of cryptic diversity, motivated this study which aimed to determine: taxonomic status and phylogenetic relationship of Plitvice Lakes trout with other Salmo species, genetic structure and diversity of their populations, as well as future viability of populations. 150 samples of trout were obtained from 17 localities. Two mitochondrial gene markers, gene for cytochrome b and mitochondrial control region were used in phylogenetic reconstruction, as well as in population genetic investigation. Intraspecific and intrapopulation genetic diversity was described by calculating several measures of genetic polymorphism, while gene flow among populations was estimated as the migration rates and as the number of immigrants per generation. Phylogenetic trees revealed that Plitvice Lakes watershed is inhabited by two evolutionary independent units of the genus Salmo, representing two species, Salmo labrax Pallas, 1814 and Salmo trutta Linnaeus, 1758. Possibly ‘pure’ populations of both species were found alongside several admixed ones in which both species are present. Our data has shown low intraspecific and intrapopulational diversities for Plitvice Lakes trout and restricted gene flow. Small effective sizes of populations and weak interconnections between them, which are most likely a consequence of fragmentation and habitat degradation, accentuating the need for urgent conservation measures. 相似文献
45.
Tihomir Tomašić Nace Zidar Veronika Rupnik Andreja Kovač Didier Blanot Stanislav Gobec Danijel Kikelj Lucija Peterlin Mašič 《Bioorganic & medicinal chemistry letters》2009,19(1):153-157
Mur ligases catalyze the biosynthesis of the UDP-MurNAc-pentapeptide precursor of peptidoglycan, an essential polymer of bacterial cell-wall. They constitute attractive targets for the development of novel antibacterial agents. Here we report on the synthesis of a series of 2,4-diaminoquinazolines, quinazoline-2,4(1H,3H)-diones, 5-benzylidenerhodanines and 5-benzylidenethiazolidine-2,4-diones and their inhibitory activities against MurD from Escherichia coli. Compounds (R)-27 and (S)-27 showed inhibitory activity against MurD with IC50 values of 174 and 206 μM, respectively, which makes them promising starting points for optimization. 相似文献
46.
Doubled haploid production in rocket (Eruca sativa Mill.) through isolated microspore culture 总被引:2,自引:0,他引:2
Lucija Leskovšek Marijana Jakše Borut Bohanec 《Plant Cell, Tissue and Organ Culture》2008,93(2):181-189
Haploid induction in rocket (Eruca sativa Mill.), a novel and increasingly important vegetable, was studied in microspore culture. A procedure based on a high sucrose
NLN medium and heat shock treatment resulted in nuclear divisions and embryo induction. The effect of genotype both among
seed lots and among single plants was a major factor influencing embryo formation. The addition of activated charcoal was
essential for obtaining reproducible results, 0.2 mg l−1 being superior to 1.0 mg l−1. A 24 h heat shock treatment at 32°C doubled the embryogenic response compared to a 48 h treatment. Embryo conversion was
only efficient (23%) for embryos that had been cultured on medium with activated charcoal and subcultured on solid B5 medium;
pretreatment of embryos with ABA or desiccation for 1–3 weeks inhibited embryo conversion. Analysis of ploidy level revealed
that the majority (65.6%) of 489 regenerated plantlets tested were diploid. Breeding programs and genetic studies of rocket
are likely to benefit substantially from the established method. 相似文献
47.
Kazem Nouri Johannes Ott Johannes C Huber Eva-Maria Fischer Lucija Stögbauer Clemens B Tempfer 《Reproductive biology and endocrinology : RB&E》2010,8(1):52