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31.
Pietro Cugini Loredana Di Palma Salvatore Di Simone Piernatale Lucia Paola Battisti Alessandro Coppola Giuseppe Leone 《Chronobiology international》1993,10(1):73-78
This study aimed to explore the 24-h patterns of stroke volume, cardiac output, and peripheral vascular resistance along with other correlated variables, such as left ventricular ejection time, ejection velocity index, thoracic fluid index, heart rate, and blood pressure. The study was performed on 12 clinically healthy subjects by means of a noninvasive beat-to-beat monitoring using the thoracic electric bioimpedance technique associated with the automated sphygmomano-metric recording. Time data series were analyzed by means of chronobiological procedures. The results documented the occurrence of a circadian rhythm for all the variables investigated, giving relevance to the beat-to-beat bioperiodicity of cardiac output and peripheral vascular resistance. Temporal quantification of the investigated variables may be useful for a better insight of the chronophysiology of the cardiovascular apparatus. 相似文献
32.
Lucia Pitzurra Manuela Puliti Mohamed Ali Burhan Fuad Francesco Bistoni Elisabetta Blasi 《FEMS immunology and medical microbiology》1993,7(4):289-295
Abstract The present study was designed to establish the susceptibility of macrophage-mediated effector functions to tetanus toxin (TT). Using the murine macrophage cell line, GG2EE, generated in vitro by v- raf /v- myc oncogenes, we have previously provided evidence that TT selectively inhibits interferon gamma (IFN-γ), but not basal, lysozyme activity. Here we show that while neither phagocytic nor candidacidal activities are affected by TT treatment, antitumoral activity is significantly impaired after exposure to TT. This phenomenon, which is dose-dependent, is fully ascribed to the holotoxin, as heat inactivated TT, C or A-B fragments result ineffective. Furthermore, C but not A-B fragment competes with TT in abrogating its inhibitory effects. Overall, these data indicate that TT is not a broad-spectrum, down-regulating signal on macrophage-mediated functions, thus implying that its toxic action is exerted on specific molecular targets. 相似文献
33.
Luca M. Neri Daniela Milani Marco Marchisio Lucia Bertolaso Fiorenzo Marinelli Francesco A. Manzoli Silvano Capitani 《Histochemistry and cell biology》1993,100(2):121-129
The rat pheochromocytoma PC12 cell line, which differentiates into sympathetic neurons under nerve growth factor (NGF) treatment, contains at least three phosphoinositidase C (PIC) isozymes, PIC , PIC , PIC . These isozymes have been previously shown to display a different subcellular localization. To determine whether or not NGF induces changes in the presence and/or distribution of PIC isozymes during PC12 neural differentiation, studies were carried out by means of in situ immunocytochemistry. After NGF administration the proliferative activity was progressively reduced to very low levels, as measured by bromodeoxyUridine incorporation, and a neuron-like morphology was displayed by almost all cells. In unstimulated PC12 cells, PIC was detected in the nucleus whereas PIC was only cytoplasmic; PIC was found in both cell compartments. In cells treated with NGF for 3 days, neural processes extended to twice the diameter of the cell body; the isoform was concentrated near the nucleus, while the immunoreactivity of the form remained constant and the form was increased. After 10 days of treatment with NGF, PIC was hardly detectable and PIC immunostaining was considerably decreased. On the contrary, PIC progressively increased and, after 14 days of NGF exposure, fully differentiated cells displayed an intense labelling of cell body and neurites. In the same cells, PIC and PIC were almost negative. These results suggest that NGF dependent neural differentiation is related to the selective down regulation of PIC and and the increase of PIC isozyme associated with the decrease of cell proliferation. 相似文献
34.
35.
Roberta Pierattelli Lucia Banci D. L. Turner 《Journal of biological inorganic chemistry》1996,1(4):320-329
The chemical shifts of several 13C nuclei positioned α to the haems in oxidised cyanide complexes of horseradish peroxidase and lignin peroxidase are reported and analysed in terms
of π molecular orbitals with perturbed D4h symmetry. The additional contributions to the paramagnetic shifts of 13C nuclei in the vinyl groups which arise from conjugation with the porphyrin π molecular orbitals are discussed, and an empirical correction factor is derived from a number of other compounds which contain
haems b. The orbital mixing parameter which is obtained from the analysis of the experimental 13C shifts is compared with the orientation of the axial histidine ligands in X-ray structures of related compounds and found
to be close to the orientation of the normal to the histidine ring. Comparison with the magnetic axes determined by fitting
the dipolar shifts of several protons which have been assigned previously also shows close agreement with the negative in-plane
rotation of the magnetic y axis. It is therefore possible to obtain the approximate orientation of the magnetic axes from 13C resonances of the haem and hence to determine the dipolar shifts at any point in space with respect to the haem by using
these axes together with the anisotropy of the magnetic susceptibility, which can be obtained by extrapolation from EPR g values. Excellent agreement is found between dipolar shifts obtained by fitting an empirical magnetic susceptibility tensor
and predictions based on 13C NMR and EPR in the case of lignin peroxidase. The agreement is less good in the case of horseradish peroxidase, in which
the empirical magnetic z axis appears to be tilted significantly away from the haem normal, though this may be due in part to the lack of accurate
atomic coordinates. It is concluded that useful estimates of the magnetic susceptibility tensor may be obtained from 13C NMR and EPR studies even in large mammalian peroxidases for which no structural models are available.
Received: 27 December 1995 / Accepted: 17 April 1996 相似文献
36.
Alberto M. Martelli Lucia Manzoli Silvia Rubbini Anna Maria Billi Renato Bareggi Lucio Cocco 《Biology of the cell / under the auspices of the European Cell Biology Organization》1995,83(1):15-22
Summary— Using two-dimensional polyacrylamide gels stained with Coomassie blue we have studied the protein composition of the nuclear matrix obtained from mouse erythroleukemic nuclei kept at O°C throughout the isolation procedure to prepare the high ionic strength resistant fraction (control matrix) or stabilized in vitro or in vivo by different procedures prior to subfractionation (ie 37°C incubation of isolated nuclei; sodium tetrathionate exposure of purified nuclei; heat shock of intact cells). When the matrix obtained from 37°C incubated nuclei was compared with the control matrix, striking differences in the polypeptide pattern were seen if the protein was obtained in both cases from an equivalent number of nuclei. On the other hand, if the same amount of protein for both the samples was applied to the gels the differences were less evident. Sodium tetrathionate stabilization of isolated nuclei and heat shock of intact cells produced a matrix protein pattern that was very similar and differed from that of the in vitro heat-exposed matrix. Using specific polyclonal antisera, we demonstrate that nucleolar proteins B23/numatrin and C23/nucleolin were very abundant in the matrix obtained from chemically-treated nuclei or in vivo heat-stabilized nuclei but were recovered in very small amounts (B23) or completely absent (C23) in the matrix prepared from nuclei heated to 37°C in vitro. Differences were seen also in the recovery of nuclear lamins, and especially lamin B, that was poorly represented in the sodium tetrathionate-stabilized matrix. The results demonstrate that in mouse erythroleukemia cells the increased recovery of nuclear matrix protein that is seen after in vitro heating of isolated nuclei is predominantly due to an additional recovery of the same types of polypeptides that are detected also in the absence of such a treatment. The data also indicate that in vivo heat shock of intact cells produces a nuclear matrix protein pattern that is more similar to the pattern seen after stabilization of purified nuclei with sodium tetrathionate and differs significantly from that obtained by exposing nuclei to 37°C in vitro, unlike to that what previous reports have indicated. 相似文献
37.
Amicucci Antonella Rossi Ismaela Potenza Lucia Agostini Deborah Stocchi Vilberto 《Biotechnology Techniques》1997,11(3):149-154
Isolates of white truffles were identified as Tuber magnatum Pico species using a pair of primers selected from a sequence characterised amplified region (SCAR) and a specific random amplified polymorphic DNA (RAPD) marker. The present study reveals that PCR-fragment-pattern polymorphisms, the construction of probes and couples of primers from one or more of these polymorphic fragments may provide a useful and rapid tool for identifying species of ectomycorrhizal fungi in addition to conventional methods (morphological parameters). 相似文献
38.
39.
V Pesce Delfino A De Lucia V Scattarella T Lettini R Lenoci V Mitolo 《Bollettino della Società italiana di biologia sperimentale》1984,60(7):1301-1307
An analytical morphometrical investigation of fronto facial profile of Plesianthropus transvaalensis was carried out by means of Fourier spectral series. Amplitude of contributors was evaluated in comparison with harmonic sets of a modern skull and two "mosaics" obtained by a convenient merging of segments of modern and hominid skull profiles. Patterns are distinctly different in Plesianthropus and modern profile because amplitude of second harmonic is greater than first in the former and viceversa in the latter; moreover while in Plesianthropus first and second harmonic give the same phase, it does not occur for modern skull. Fourier series of "mosaic" obtained by frontal profile of Plesianthropus and facial profile of modern resembles the set of modern skull while "mosaic" of modern frontal profile and hominid facial one resembles closely pattern of Plesianthropus. 相似文献
40.
The sporophyte foot of the mossTimmiella barbuloides consists of an unistratose epidermis of transfer cells, a parenchymatous cortex, and a small central strand consisting only of hydroids. The parenchymatous tissue of the vaginula develops one layer of transfer cells opposite the foot, whose lower extremity extends into the gametophyte stem's central strand. From the bottom to the top of the foot the ultrastructure of the sporophyte transfer cells shows some gradual changes that appear related to a functional specialization of these cells. According to a centripetal gradient, the quantity of plastid starch progressively lessens in both vaginula parenchyma and foot cortex. the observed morphological patterns suggest that in the foot-vaginula complex nutrients are translocated radially up to the sporophyte central strand. 相似文献