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81.
Serra AL Mariscotti JF Barra JL Lucchesi GI Domenech CE Lisa AT 《Journal of bacteriology》2002,184(15):4301-4303
The gene for glycine betaine transmethylase (gbt) was identified in Pseudomonas aeruginosa strain Fildes III by biochemical, physiological, and molecular approaches. Based on sequence analysis, the knockout gene corresponded to an open reading frame (ORF) named PA3082 in the genome of P. aeruginosa PAO1. The translated product of this ORF displayed similarity to transferases of different microorganisms. Mutation in gbt blocked the utilization of choline and glycine betaine as carbon and nitrogen sources. 相似文献
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84.
The female gonad of Prorhynchus is heterocellular (neoophoran organization) and consists of an unpaired, elongate germovitellarium enveloped by a finely granular extracellular lamina. It is composed of a posterior germinative area where early oocytes are randomly associated with differentiating vitellocytes and a growth area with follicular organization. In each follicle a single oocyte is surrounded by a layer of vitellocytes. By electron microscopy, the oocytes showed features typical of non-vitellogenic germ cells; they had chromatoid bodies, annulate lamellae, lipid droplets and R.E.R. and Golgi complexes producing small granules with a multilamellar pattern. Vitellocytes showed features typical of secretory cells with the R.E.R. and Golgi complex developed to a great extent and involved in the production of type A and type B globules, respectively. We speculate that type A globules are shell-globules and type B globules are yolk. The structure, composition and role of vitellocyte globules of Prorhynchus are compared with those of homologous inclusions from other Platyhelminthes.Abbreviations A
type A globule
- B
type B globule
- ECL
extracellular lamina
- GC
Golgi complex
- L
lipid
- RER
rough endoplasmic reticulum
- O
oocyte
- V
vitellocyte 相似文献
85.
Two Daphnia clones were isolated from different day depths during the period of diel vertical migration and were tested for their life-history responses to a fish exudate released by juvenile perch. Animals were exposed to fish exudate every 24 or 48 h. Both clones responded to the exudate by exhibiting earlier maturation and larger sizes of first clutches, which resulted in higher rates of population increase. Also, neonates were smaller in the presence of the exudate. It was found that the clone isolated from a deeper day depth (migrating clone) was less sensitive to the exudate than the clone isolated from the surface waters, which was presumed to be non-migrating. The non-migrating clone responded by having smaller neonate sizes and smaller sizes at maturity than the migrating clone. The non-migrating clone responded to the fish chemical when it was exposed to it every 24 or 48 h, whereas the migrating clone only responded to the exudate if exposed to it every 24 h. 相似文献
86.
Contractile activity is required for sarcomeric assembly in phenylephrine-induced cardiac myocyte hypertrophy 总被引:1,自引:0,他引:1
Eble Diane M.; Qi Ming; Waldschmidt Stephanie; Lucchesi Pamela A.; Byron Kenneth L.; Samarel Allen M. 《American journal of physiology. Cell physiology》1998,274(5):C1226
Agonist-inducedhypertrophy of cultured neonatal rat ventricular myocytes (NRVM) hasbeen attributed to biochemical signals generated during receptoractivation. However, NRVM hypertrophy can also be induced byspontaneous or electrically stimulated contractile activity in theabsence of exogenous neurohormonal stimuli. Using single-cell imagingof fura 2-loaded myocytes, we found that low-density, noncontractingNRVM begin to generate intracellularCa2+ concentration([Ca2+]i)transients and contractile activity within minutes of exposure to the1-adrenergic agonistphenylephrine (PE; 50 µM). However, NRVM pretreated with verapamiland then stimulated with PE failed to elicit[Ca2+]itransients and beating. We therefore examined whether PE-induced [Ca2+]itransients and contractile activity were required to elicit specificaspects of the hypertrophic phenotype. PE treatment (48-72 h)increased cell size, total protein content, total protein-to-DNA ratio,and myosin heavy chain (MHC) isoenzyme content. PE also stimulatedsarcomeric protein assembly and prolonged MHC half-life. However,blockade of voltage-gated L-typeCa2+ channels with verapamil,diltiazem, or nifedipine (10 µM) blocked PE-induced total protein andMHC accumulation and prevented the time-dependent assembly ofmyofibrillar proteins into sarcomeres. Inhibition of actin-myosincross-bridge cycling with 2,3-butanedione monoxime (7.5 mM) alsoprevented PE-induced total protein and MHC accumulation, indicatingthat mechanical activity, rather than[Ca2+]itransients per se, was required. In contrast, blockade of[Ca2+]itransients and contractile activity did not prevent the PE-induced increase in cell surface area, activation of the mitogen-activated protein kinases ERK1 and ERK2, or upregulation of atrial natriuretic factor gene expression. Thus contractile activity is required to elicitsome but not all aspects of the the hypertrophic phenotype induced by1-adrenergic receptoractivation. 相似文献
87.
Considerable attention is being given to the interactions that occur among blood platelets, neutrophils, and the vascular endothelium. There is an increasing awareness that the various blood elements interact in the process of thrombus formation and vascular occlusion. In addition, interactions among these cells can lead to the formation and release of vasoactive substances that have the potential to modulate regional blood flow. This review focuses on the coronary vascular bed and an assessment of how cell-cell interactions, under normal physiological conditions as well as in the presence of myocardial injury, may lead to alterations in coronary vascular resistance and myocardial function. Should related events be operative in human clinical states of disease, the circulating elements of the blood may serve as targets in the development of therapeutic interventions to regulate myocardial blood flow. 相似文献
88.
Free radical scavengers in myocardial ischemia 总被引:7,自引:0,他引:7
Reperfusion of ischemic myocardium is recognized as potentially beneficial because mortality is directly related to infarct size, and the latter is related to the severity and duration of ischemia. However, reperfusion is associated with extension of the injury that is additive to that produced by ischemia alone. The phenomenon of reperfusion injury is caused in large part by oxygen-derived free radicals from both extracellular and intracellular sources. The loci of oxygen-free radical formation include: myocardial sources (mitochondria), vascular endothelial sources (xanthine oxidase and other oxidases), or the inflammatory cellular infiltrate (neutrophils). Experimental studies have shown that free radical scavengers and agents that prevent free radical production can reduce myocardial infarct size in dogs subjected to temporary regional ischemia followed by reperfusion. Superoxide dismutase and catalase, which catalyze the breakdown of superoxide anion and hydrogen peroxide, respectively, limit experimental myocardial infarct size. The free radical scavenging agent N-(2-mercaptopropionyl)glycine (MPG) is reported to be effective in limiting infarct size. The ischemic-reperfused myocardium derives significant protection when experimental animals are pretreated with the xanthine oxidase inhibitor allopurinol. Neutrophils also serve as a significant source of oxygen-derived free radicals at the site of tissue injury. A number of agents have been shown to directly inhibit neutrophil-derived oxygen free radical formation and neutrophil accumulation within the reperfused myocardium. These agents include ibuprofen, nafazatrom, BW755C, prostacyclin, and iloprost. Thus, free radical scavengers and agents that prevent free radical formation can provide significant protection to the ischemic-reperfused myocardium. 相似文献
89.
The location of sequences homologous to a cloned D. melanogaster DNA segment, Dm 25, has been examined in polytene chromosomes by hybridization in situ. Dm 25 localizes to multiple sites and shows variation in patterns between different strains and among individuals within wild-type laboratory strains. Analysis of numerous geographically distinct isogenic lines suggests that Dm 25 patterns are determined by germ-line factors and are not the product of strictly somatic events. In general there is wide variation in Dm 25 patterns among different lines, but a significant number of sites are common to two or more distinct lines. Hybridization to restriction digests of genomic DNA suggests that Dm 25 is a moderately repetitive, conserved sequence whose copies are dispersed throughout the genome. Analysis of species other than melanogaster indicates a significant divergence in structure of sequences homologous to Dm 25 as well as a drastic reduction in amount of homology to the melanogaster sequence. 相似文献
90.
Regulation of -1 ribosomal frameshifting directed by cocksfoot mottle sobemovirus genome. 总被引:3,自引:0,他引:3
J Lucchesi K M?kel?inen A Merits T Tamm K M?kinen 《European journal of biochemistry》2000,267(12):3523-3529