Short-term consequences of slash-and-burn practices on the arbuscular mycorrhizal fungi of a tropical dry forest

Rates of land conversion from forest to cultivated land by slash-and-burn practices are higher in tropical dry forest (TDF) than any other Neotropical forest type. This study examined the short-term consequences of the slash-and-burn process on arbuscular mycorrhizal fungi (AMF). We expected that slash-and-burn would reduce mycorrhizal colonization and propagules and change species richness and composition. Soil and root samples were taken from TDF control and pasture plots originated after slash-and-burn at four dates during the year of conversion to examine species composition, spore abundance, and infective propagules. Additionally, spore abundance and viability and viable intraradical colonization were measured twice during the second year after conversion. Forest and pasture plots maintained similar species richness and an overall 84% similarity during the first year after conversion. Infective propagules were reduced in pasture plots during the first year after slash-and-burn, whereas spore abundance and intraradical colonization remained similar in TDF and pasture plots both years of the study. Our results suggest, contrary to the expected, that forest conversion by means of slash-and-burn followed by cultivation resulted in few immediate changes in the AMF communities, likely because of the low heat conductivity of the soil and rapid combustion of plant residues.     

Colonic luminal ammonia and portal blood l-glutamine and l-arginine concentrations: a possible link between colon mucosa and liver ureagenesis

The highest ammonia concentration in the body is found in the colon lumen and although there is evidence that this metabolite can be absorbed through the colonic epithelium, there is little information on the capacity of the colonic mucosa to transfer and metabolize this compound. In the present study, we used a model of conscious pig with a canula implanted into the proximal colon to inject endoluminally increasing amounts of ammonium chloride and to measure during 5 h the kinetics of ammonia and amino acid concentration changes in the portal and arterial blood. By injecting as a single dose from 1 to 5 g ammonia into the colonic lumen, a dose-related increase in ammonia concentration in the portal blood was recorded. Ammonia concentration remained unchanged in the arterial blood except for the highest dose tested, i.e. 5 g which thus apparently exceeds the hepatic ureagenesis capacity. By calculating the apparent net ammonia absorption, it was determined that the pig colonic epithelium has the capacity to absorb 4 g ammonia. Ammonia absorption through the colonic epithelium was concomitant with increase of l-glutamine and l-arginine concentrations in the portal blood. This coincided with the expression of both glutamate dehydrogenase and glutamine synthetase in isolated colonic epithelial cells. Since l-glutamine and l-arginine are known to represent activators for liver ureagenesis, we propose that increased portal concentrations of these amino acids following increased ammonia colonic luminal concentration represent a metabolic link between colon mucosa and liver urea biosynthesis.     

Contrasting palm species and use diversity in the Yucatan Peninsula and the Ecuadorian Amazon

We analyze the relationship between palm species diversity and diversity of palm use in two areas (Amazonian Ecuador; Yucatan Peninsula) of equivalent size but with contrasting characteristics in palm species diversity and morphology, and in the phylogenetic composition of palm flora. The areas also differ in their cultural and socioeconomic contexts. Palm use diversity is significantly higher in the Ecuadorian Amazon than in the Yucatan Peninsula and the lower species diversity of palms in the Yucatan Peninsula is not matched by a more intensive use of the fewer species found there. The taxonomic composition of the palm flora is a poor predictor of extent of use and morphological characteristics of palm species affect usefulness only in the Ecuadorian Amazon. The Yucatecans’ more limited reliance on forest products might explain the observed patterns. Ornamental palm use is an indicator of a general change in plant use patterns associated with tourism and macro-economic development in the Yucatan Peninsula. We find a positive relationship between ecosystem plant diversity and plant use diversity, but socioeconomic factors such as market integration strongly influence the use of local biodiversity. Palms represent a diverse and important natural resource that deserves further investigation to secure its sustainable management and conservation in the two studied sites, regardless of their degree of market integration.     

Membrane binding of lipidated Ras peptides and proteins--the structural point of view

Biological membranes are interesting interfaces, at which important biological processes occur. In addition to integral membrane proteins, a number of proteins bind to the membrane surface and associate with it. Posttranslational lipid modification is one important mechanism, by which soluble molecules develop a propensity towards the membrane and reversibly bind to it. Membrane binding by insertion of hydrophobic lipid moieties is relevant for up to 10% of all cellular proteins. A particular interesting lipid-modified protein is the small GTPase Ras, which plays a key role in cellular signal transduction. Until recently, the structural basis for membrane binding of Ras was not well-defined. However, with the advent of new synthesis techniques and the advancement of several biophysical methods, a number of structural and dynamical features about membrane binding of Ras proteins have been revealed. This review will summarize the chemical biology of Ras and discuss in more detail the biophysical and structural features of the membrane bound C-terminus of the protein.     

Gene electrotransfer: from biophysical mechanisms to in vivo applications

Gene electrotransfer can be obtained not just on single cells in diluted suspension. For more than 10 years, this is a quasi routine strategy in tissue on the living animal and a few clinical trials have now been approved. New problems have been brought by the close contacts of cells in tissue both on the local field distribution and on the access of DNA to target cells. They need to be solved to provide a further improvement in the efficacy and safety of protein expression. There is a competition between gene transfer and cell destruction. Nevertheless, present results are indicative that electrotransfer is a promising approach for gene therapy. High level and long-lived expression of proteins can be obtained in muscles. This is used for a successful method of electrovaccination.     

The Differential Interaction of Brucella and Ochrobactrum with Innate Immunity Reveals Traits Related to the Evolution of Stealthy Pathogens

Background

During evolution, innate immunity has been tuned to recognize pathogen-associated molecular patterns. However, some α-Proteobacteria are stealthy intracellular pathogens not readily detected by this system. Brucella members follow this strategy and are highly virulent, but other Brucellaceae like Ochrobactrum are rhizosphere inhabitants and only opportunistic pathogens. To gain insight into the emergence of the stealthy strategy, we compared these two phylogenetically close but biologically divergent bacteria.

Methodology/Principal Findings

In contrast to Brucella abortus, Ochrobactrum anthropi did not replicate within professional and non-professional phagocytes and, whereas neutrophils had a limited action on B. abortus, they were essential to control O. anthropi infections. O. anthropi triggered proinflammatory responses markedly lower than Salmonella enterica but higher than B. abortus. In macrophages and dendritic cells, the corresponding lipopolysaccharides reproduced these grades of activation, and binding of O. anthropi lipopolysaccharide to the TLR4 co-receptor MD-2 and NF-κB induction laid between those of B. abortus and enteric bacteria lipopolysaccharides. These differences correlate with reported variations in lipopolysaccharide core sugars, sensitivity to bactericidal peptides and outer membrane permeability.

Conclusions/Significance

The results suggest that Brucellaceae ancestors carried molecules not readily recognized by innate immunity, so that non-drastic variations led to the emergence of stealthy intracellular parasites. They also suggest that some critical envelope properties, like selective permeability, are profoundly altered upon modification of pathogen-associated molecular patterns, and that this represents a further adaptation to the host. It is proposed that this adaptive trend is relevant in other intracellular α-Proteobacteria like Bartonella, Rickettsia, Anaplasma, Ehrlichia and Wolbachia.