Phosphatase activities of cyanobacteria as indicators of nutrient status in a Pyrenees river

There is increasing evidence that fluvial systems are influenced by anthropogenic factors, and disturbance due to pollution and other human interference gives rise to specific problems. It is now imperative that we develop and apply novel and effective ways which allow us to monitor water quality. The present study was planned as part of a programme to develop biological monitoring methods to assess nutrient characteristics of upland calcareous streams and rivers. Phototrophs respond to environmental changes over a period of time, so organisms sampled at one time can potentially provide almost as much information about nutrients in the water as a number of individual chemical measurements. Phosphatase activity is often a good indicator of phosphorus limitation, and field materials could be used to study changes in nutrients dynamics. The calcareous River Muga, north-east Spain, at a site 10 km downstream from its source in the Pyrenees, was therefore chosen for the present study of surface phosphatase activities of the main cyanobacterial communities at different seasons to assess P limitation and establish the suitability of this method for use in monitoring catchment processes. Here we report seasonal changes of phosphatase activity in field populations of Schizothrix coriacea, Rivularia biasolettiana, Tolypothrix distorta var. penicillata and Nostoc verrucosum. All four cyanobacteria showed marked surface phosphomonoesterase and phosphodiesterase activity on each sampling period. Michaelis-Menten kinetic studies showed similar K _m values for the four species suggesting similar affinity for organic P substrates. Light had no effect on phosphatase activities, indicating that there is no need to consider this factor in short-term field assays. However, there was an increase in phosphomonoesterase activity of Rivularia with rise in temperature over the range 10–35°C, which suggests adaptation to the frequent temperature changes in nature. Phosphorus limitation seems the main chemical factor influencing phototrophs in R. Muga. Combined observations on macroscopically visible phototrophs with assays of surface phosphatase activity provide a valuable means of assessing long-term changes in a catchment.     

Repetitively rated plasma relativistic microwave oscillator with a controllable frequency in every pulse

A repetitively rated microwave oscillator whose frequency can be varied electronically from pulse to pulse in a predetermined manner is created for the first time. The microwave oscillator has a power on the order of 10⁸ W and is based on the Cherenkov interaction of a high-current relativistic electron beam with a plasma preformed before each pulse. Electronic control over the plasma properties allows one to arbitrarily vary the microwave frequency from pulse to pulse at a pulse repetition rate of up to 50 Hz.     

ACUTE EFFECTS OF A RESISTED DYNAMIC WARM-UP PROTOCOL ON JUMPING PERFORMANCE

This study aimed to investigate the kinematic and kinetic changes when resistance is applied in horizontal and vertical directions, produced by using different percentages of body weight, caused by jumping movements during a dynamic warm-up. The group of subjects consisted of 35 voluntary male athletes (19 basketball and 16 volleyball players; age: 23.4 ± 1.4 years, training experience: 9.6 ± 2.7 years; height: 177.2 ± 5.7 cm, body weight: 69.9 ± 6.9 kg) studying Physical Education, who had a jump training background and who were training for 2 hours, on 4 days in a week. A dynamic warm-up protocol containing seven specific resistance movements with specific resistance corresponding to different percentages of body weight (2%, 4%, 6%, 8%, 10%) was applied randomly on non consecutive days. Effects of different warm-up protocols were assessed by pre-/post- exercise changes in jump height in the countermovement jump (CMJ) and the squat jump (SJ) measured using a force platform and changes in hip and knee joint angles at the end of the eccentric phase measured using a video camera. A significant increase in jump height was observed in the dynamic resistance warm-up conducted with different percentages of body weight (p < 0.05). On the other hand, no significant difference in different percentages of body weight states was observed (p > 0.05). In jump movements before and after the warm-up, while no significant difference between the vertical ground reaction forces applied by athletes was observed (p > 0.05), in some cases of resistance, a significant reduction was observed in hip and knee joint angles (p < 0.05). The dynamic resistance warm-up method was found to cause changes in the kinematics of jumping movements, as well as an increase in jump height values. As a result, dynamic warm-up exercises could be applicable in cases of resistance corresponding to 6-10% of body weight applied in horizontal and vertical directions in order to increase the jump performance acutely.     

Rho1 regulates adherens junction remodeling by promoting recycling endosome formation through activation of myosin II

Once adherens junctions (AJs) are formed between polarized epithelial cells they must be maintained because AJs are constantly remodeled in dynamic epithelia. AJ maintenance involves endocytosis and subsequent recycling of E-cadherin to a precise location along the basolateral membrane. In the Drosophila pupal eye epithelium, Rho1 GTPase regulates AJ remodeling through Drosophila E-cadherin (DE-cadherin) endocytosis by limiting Cdc42/Par6/aPKC complex activity. We demonstrate that Rho1 also influences AJ remodeling by regulating the formation of DE-cadherin–containing, Rab11-positive recycling endosomes in Drosophila postmitotic pupal eye epithelia. This effect of Rho1 is mediated through Rok-dependent, but not MLCK-dependent, stimulation of myosin II activity yet independent of its effects upon actin remodeling. Both Rho1 and pMLC localize on endosomal vesicles, suggesting that Rho1 might regulate the formation of recycling endosomes through localized myosin II activation. This work identifies spatially distinct functions for Rho1 in the regulation of DE-cadherin–containing vesicular trafficking during AJ remodeling in live epithelia.     

Molecular Fingerprinting of Cyanobacteria from River Biofilms as a Water Quality Monitoring Tool

Benthic cyanobacterial communities from Guadarrama River (Spain) biofilms were examined using temperature gradient gel electrophoresis (TGGE), comparing the results with microscopic analyses of field-fixed samples and the genetic characterization of cultured isolates from the river. Changes in the structure and composition of cyanobacterial communities and their possible association with eutrophication in the river downstream were studied by examining complex TGGE patterns, band extraction, and subsequent sequencing of 16S rRNA gene fragments. Band profiles differed among sampling sites depending on differences in water quality. The results showed that TGGE band richness decreased in a downstream direction, and there was a clear clustering of phylotypes on the basis of their origins from different locations according to their ecological requirements. Multivariate analyses (cluster analysis and canonical correspondence analysis) corroborated these differences. Results were consistent with those obtained from microscopic observations of field-fixed samples. According to the phylogenetic analysis, morphotypes observed in natural samples were the most common phylotypes in the TGGE sequences. These phylotypes were closely related to Chamaesiphon, Aphanocapsa, Pleurocapsa, Cyanobium, Pseudanabaena, Phormidium, and Leptolyngbya. Differences in the populations in response to environmental variables, principally nutrient concentrations (dissolved inorganic nitrogen and soluble reactive phosphorus), were found. Some phylotypes were associated with low nutrient concentrations and high levels of dissolved oxygen, while other phylotypes were associated with eutrophic-hypertrophic conditions. These results support the view that once a community has been characterized and its genetic fingerprint obtained, this technique could be used for the purpose of monitoring rivers.