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91.
René Rezsöhazy Bernard Hallet Jean Delcour Jacques Mahillon 《Molecular microbiology》1993,9(6):1283-1295
The eight IS 231 variants characterized so far (IS 231 A-F, V and W) display similar transposases with an overall 40% identity. Comparison with all the proka-ryotic transposable elements sequenced so far revealed that the IS231 transposases share two conserved regions with those of 35 other insertion sequences of wide origins. These insertion sequences, defining the IS4 family, have a common bipartite organization of their ends and are divided into two similarity groups. Interestingly, the transposase domains conserved within this family display similarities with the well known integrase domain shared by transposases of the IS3 and IS15 families, and integrases of retroelements. This domain is also found in IS30- related elements and Tn7 TnsB protein. Amino acid residues conserved throughout all these prokaryotic and eukaryotic mobile genetic elements define a major transposase/integrase motif, likely to play an important role in the transposition process. 相似文献
92.
Jacques Vasse Françoise de Billy Georges Truchet 《The Plant journal : for cell and molecular biology》1993,4(3):555-566
Nodulation, the organogenetic process resulting from the symbiotic interaction between Rhizobium and legumes, is under the feedback control of the plant. However, the autoregulatory mechanisms controlling root nodule formation are poorly understood. In this paper it is shown that alfalfa can react to infection by its symbiont Rhizobium meliloti by eliciting a defence mechanism similar to the hypersensitive reaction (HR) observed in incompatible plant-pathogen interactions. After the first nodule primordia have been induced, an increasing proportion of infection threads abort in a single or a few root cortical cells in which both symbionts simultaneously undergo necrosis. Autofluorescent, cytochemical and immunolocalization assays revealed that phenolic compounds and proteins associated with defence mechanisms in plants have accumulated in the necrotic cells. These results lead to the proposition that the elicitation of a HR is part of the mechanism by which the plant controls infection and, therefore, regulates nodulation. 相似文献
93.
Phenotypic effects of overexpression of Agrobacterium rhizogenes T-DNA ORF13 in transgenic tobacco plants are mediated by diffusible factor(s) 总被引:1,自引:1,他引:0
Geneviève Hansen Danièle Vaubert Jean Noël Héron Danielle Clérot Jacques Tempe Jean Brevet 《The Plant journal : for cell and molecular biology》1993,4(3):581-585
Nicotiana tabacum cv. Xanthi transgenic plants expressing ORF13 of Agrobacterium rhizogenes 8196 T-DNA under the 35S RNA promoter from the cauliflower mosaic virus displayed developmental abnormalities. They were small, with short and variable internodal lengths, their root systems were poorly developed; leaves were small, asymmetric, rounded, wrinkled and dark green; flowers were short, and irregularly shaped. They exhibited reduced apical dominance and regularly produced offshoots at the base of the plant. This phenotype was also exhibited by offshoots of normal N. tabacum cv. Xanthi stock grafted with a transgenic scion indicating that expression of ORF13 influences plant development via diffusible factor(s). 相似文献
94.
Summary N6-benzyl-adenine (BA) enhanced phyllogenesis and axillary bud development of Paeonia suffruticosa during in vitro culture allowing good propagation while N6-(2isopentenyl)adenine (iP) did not. During the first five days of culture, the mitotic activity of BA-treated explants was higher than in the iP-treated ones. High BA levels were detected in the BA-treated explants, and this was correlated with the absence of or the low indole-3-acetic acid (IAA) content. The low iP levels measured in iP-treated explants were correlated with high endogenous IAA content; the new cytokinin / auxin ratio could explain the lack of axillary buds and the development of only one leaf. Abscisic acid (ABA) was detected neither in the controls nor in the cytokinin-treated explants during the first week. However, intensive restoration of ABA accumulation was observed in controls from the third week onwards. Both BA and iP-treated explants accumulated less ABA than the controls but this hormone appeared later in the BA-treated explants than in the iP-treated ones.Abbreviations ABA
abscisic acid
- BA
N6-benzyl-adenine
- BHT
butyl-hydroxy-toluene
- ELISA
enzyme linked immunosorbent assay
- FM
fresh mass
- HPLC
high performance liquid chromatography
- IAA
indole-3-acetic acid
- iP
N6-(2-isopentenyl)adenine
- MI
mitotic index
- 9RBA
9-ß-D-ribofuranosyl-BA
- 9RiP
9-ß-Dribofuranosyl-iP
- 9RZ
9-ß-D-ribofuranosyl-zeatin
- Z
zeatin 相似文献
95.
Sinlan Poo Ana Karen Candia Kristina L. Cohen Francesca T. Erickson Sara A. Mason Bradley D. Nissen Adair F. McNear Jonathon J. Reinig Joseph S. Sherrock Ashley R. Aguiluz Letitia L. Jacques Hanna E. R. Jenkins Anne Devan-Song 《Biotropica》2023,55(4):806-815
Environmentally cued hatching has been well-documented in amphibians in response to a wide range of abiotic and biotic factors. The hatching of terrestrial amphibian eggs in response to flooding may be basal within the group, but amphibian lineages in tropical Asia and sub-Saharan Africa have not received as much attention as their Neotropical counterparts. We investigated submergence-induced hatching in Feihyla hansenae, a Rhacophorid tree frog with terrestrial eggs. We quantified natural rates of clutch submergence at our study site in Thailand. Using submergence experiments, we found that embryos are capable of hatching early to escape flooding, and that failure to hatch results in mortality. Among the embryos that were able to hatch early, only the earliest, youngest hatchlings experienced a trade-off in body size that persisted for 6 days, while later, older hatchlings were not significantly smaller than spontaneous hatchlings under control conditions. By incorporating our natural and experimental data into Monte Carlo methods to simulate and compare survival probabilities with and without hatching plasticity, we found an overall 3.1% increase in submergence survival due to hatching plasticity. Our findings support the idea that flooding-induced hatching is widespread across amphibians with terrestrial eggs and highlight the importance of researching understudied tropical regions. As climate change is projected to affect rainfall patterns, the ability of embryos to escape abiotic egg-stage threats may be an indicator of species' ability to flexibly navigate a changing environment. 相似文献
96.
Distribution and conservation of sequences homologous to the 1731 retrotransposon in Drosophila 总被引:3,自引:1,他引:2
Montchamp-Moreau C; Ronsseray S; Jacques M; Lehmann M; Anxolabehere D 《Molecular biology and evolution》1993,10(4):791-803
The distribution of 1731 retrotransposon-hybridizing sequences in the
family Drosophilidae has been studied using a 1731 probe from Drosophila
melanogaster. Squash blot and Southern blot analyses of 42 species reveal
that the 1731 sequences are widespread within both the Sophophora and
Drosophila subgenera and are also present in the genera Scaptomyza and
Zaprionus. Hence the 1731 retrotransposon family appears to have a long
evolutionary history in the Drosophilidae genome. Differences of
hybridization signal intensity suggested that the 1731 sequence is well
conserved only in the three species most closely related to D. melanogaster
(D. simulans, D. mauritiana, and D. sechellia). A survey of insertion sites
in numerous different populations of the previous four species by in situ
hybridization to polytene chromosomes has shown in all cases both
chromocentric hybridizations and a low number of sites (0-5) on the
chromosomal arms. This number of sites is among the lowest observed in D.
melanogaster and D. simulans when 1731 is compared with other
retrotransposon families. In addition, we have observed species-specific
patterns of the chromocentric hybridization signal, suggesting rapid
modifications of the beta-heterochromatin components since the radiation of
the melanogaster subgroup.
相似文献
97.
Parasites are known to alter the behavior of their hosts, but little is known about their effects on responses to sexual stimuli. Periplaneta americanainfected with the acanthocephalan Moniliformis moniliformiswere compared to uninfected animals in their behavioral and electroantennogram responses to a synthetic P. americanapheromone component, periplanone-B, and a pheromone mimic, bornyl acetate. In a t-maze there was no significant difference between infected animals' responses to periplanone-B and a random binomial distribution; the responses of uninfected animals were significantly nonrandom. The electroan-tennogram responses of infected and uninfected animals to bornyl acetate or periplanone-B did not differ significantly, however, indicating that the alteration probably does not occur at the peripheral level but at a central nervous system level. 相似文献
98.
Molecular analysis of the Lactococcus lactis subspecies lactis CNRZ270 bidirectional theta replicating lactose plasmid pUCL22 总被引:1,自引:0,他引:1
pUCL22 is the lactose protease plasmid of Lactococcus lactis ssp. lactis CNRZ270. The nucleotide sequence of its replication region Rep22 contains a non-transcribed region, the replication origin, followed by a gene encoding a putative 388-amino-acid protein named Rep22A. The promoter regions of the rep22A and pC194 cat genes share strong similarities and the pUCL22 replicon exerted trans or cis negative control on the pC194 cat gene expression in L. lactis. We suggest that Rep22A binds to its own promoter as well as to the pC194 cat promoter and thus is autoregulated. We show that pUCL22 replicates mainly by a bidirectional theta mechanism in L. lactis, and is representative of a widely distributed replicon family, members of which could be co-resident. We propose that compatibility between these closely related replicons results from minor replication protein modifications coupled with base changes in their respective binding sites, supporting the co-existence of numerous related replicons in lactococcal strains. 相似文献
99.
The distribution of NO3? reduction between roots and shoots was studied in hydro-ponically-grown peach-tree seedlings (Prunus persica L.) during recovery from N starvation. Uptake, translocation and reduction of NO3?, together with transport through xylem and phloem of the newly reduced N were estimated, using 15N labellings, in intact plants supplied for 90 h with 0.5 mM NH4+ and 0.5, 1.5 or 10 mM NO3?. Xylem transport of NO3? was further investigated by xylem sap analysis in a similar experiment. The roots were the main site of NO3? reduction at all 3 levels of NO3? nutrition. However, the contribution of the shoots to the whole plant NO3? reduction increased with increasing external NO3? availability. This contribution was estimated to be 20, 23 and 42% of the total assimilation at 0.5, 1.5 and 10 mM NO3?, respectively. Both 15N results and xylem sap analysis confirmed that this trend was due to an enhancement of NO3? translocation from roots to shoots. It is proposed that the lack of NO3? export to the shoots at low NO3? uptake rate resulted from a competition between NO3? reduction in the root epidermis/cortex and NO3? diffusion to the stele. On the other hand, net xylem transport of newly reduced N was very efficient since ca 70% of the amino acids synthesized in the roots were translocated to the shoots, regardless of the level of NO3? nutrition. This net xylem transport by far exceeded the net downward phloem transport of the reduced N assimilated in shoots. As a consequence, the reduced N resulting from NO3? assimilation, principally occurring in the roots, was mainly incorporated in the shoots. 相似文献
100.
The small-subunit ribosomal DNA (rDNA) coding sequence was determined for the help Costaris Costata (Phaeophyta) and compared to those of chlorophyll a + b- and chlorophyll a + c-containing vascular and nonvascular plants. Phylogenetic comparison of all sequences indicated a common ancestor for phaeophytes, chrysophytes and oomycetes. Phylogenies based on rDNA sequence data and those based on plastid characteristics were compared. Relative evolutionary distances between some taxa, derived from rDNA sequence data, conflicted with findings of plastid-based phylogenies. 相似文献