Qualitative yeast enzyme analysis by electrophoresis

Summary Baker's yeast (Saccharomyces cerevisiae) was cultivated under different intensities of aeration on glucose and on ethanol. Seventeen enzymes of the Embden-Meyerhof pathway and the TCA cycle or related reactions were then assayed by starch gel electrophoresis. There were both qualitative and quantitative differences in many enzymes, most notably in glyceraldehyde-3-phosphate dehydrogenase, alcohol dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase, and fumarase. Enzyme electrophoresis seems to offer a promising method for rapidly obtaining information about many yeast enzymes from a large number of samples.     

D-xylose catabolism in fusarium oxysporum

Summary The initial steps of D-xylose catabolism inFusarium oxysporum have been studied. The presence of the oxidoreductase pathway for D-xylose catabolism was demonstrated. The enzymes involved, D-xylose reductase and xylitol dehydrogenase, were found to be inducible and relatively specific for D-xylose and xylitol. D-xylose isomerase was not detected.     

The cell-envelope glycolipids of baker''s yeast

Sphingolipids were found to dominate in the glycolipids from the cell envelope of baker's yeast. A relatively large quantity of ceramides was detected. Among the several complex phosphosphingolipids described, ceramide-(P-inositol)(2)-mannose was the main component. About 55% of long-chain bases in sphingolipids consisted of C(18)-phytosphingosine (4D-hydroxysphinganine). Other bases, found in decreasing concentrations, were C(20)-phytosphingosine, C(20)-dehydrophytosphingosine, C(18)-dihydrosphingosine (sphinganine) and C(19)-dihydrosphingosine. The presence of sterol glycosides, sulpholipids, cerebrosides and acylglucoses was demonstrated.     

Über die Anpassung der Bäckerhefe an Galaktose

Zusammenfassung Die mit Einsporen-Kulturen der Bäckerhefe durchgeführten Versuche weisen darauf hin, daß die untersuchten homozygotischen Bäckerhefestämme durch Anpassung imstande waren, Galaktose zu vergären, so daß diese Erscheinung mit der genotypischen Variation nichts zu tun hat.     

Der Chromosomencyclus von Macrosiphum pisi Kalt (Aphididae)

Ohne Zusammenfassung