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Gennari L Felletti M Blasa M Angelino D Celeghini C Corallini A Ninfali P 《Phytochemical analysis : PCA》2011,22(3):272-279
Introduction – Beta vulgaris var. cicla (BV) leaves contain chemopreventive compounds that have been investigated for new drug discovery. These compounds belong to the family of the apigenin‐glycosides. Since the leaves are seasonal products containing high percentages of water, they are easily degradable during storage in fresh conditions. To be stored they require a drying process, consuming time and a large amount of energy. The extraction of apigenin‐glycosides may also be conveniently performed from BV seeds, which represent a stable and year‐long available biomass. Objectives – The present report was undertaken to find a strategy of purification of bioactive flavonoids from BV seeds and test their ability to inhibit proliferation both on human colon cancer (RKO) cells and normal human fibroblasts (HF). Materials and methods – The ethyl‐acetate extract of BV seeds was fractionated on a Sephadex LH 20 column. A fraction of this extract, labeled as P4, exploited a marked antiproliferative activity on RKO cells. The components of P4 were purified on an RP18 column chromatography and identified by HPLC‐ESI‐MS as 2,4,5‐trihydroxybenzaldehyde, 2,5‐dihydroxybenzaldehyde, vanillic acid, xylosylvitexin, glucopyranosyl‐glucopyrasyl‐rhamnetin and glucopyranosyl‐xylosyl‐rhamnetin. All of them were tested for cytostatic and cytotoxic activity on RKO and HF cells. Results – Xylosylvitexin exhibited the strongest antiproliferative activity on RKO cells, together with an enhancement of the apoptosis, an increase of cells in the G1 phase and a reduction of cells in the S phase; on the contrary, the proliferation of HF was significantly stimulated. Conclusion – Xylosylvitexin is the main and more efficient chemopreventive compound in BV seeds, but the natural cocktail of molecules, represented by P4 fraction, showed a better compromise between the antiproliferative activity on RKO cells and the enhancement of HF proliferation. Copyright © 2011 John Wiley & Sons, Ltd. 相似文献
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Dusio GF Cardani D Zanobbio L Mantovani M Luchini P Battini L Galli V Diana A Balsari A Rumio C 《Immunology and cell biology》2011,89(5):630-639
The innate immune system is present throughout the female reproductive tract and functions in synchrony with the adaptive immune system to provide protection in a way that enhances the chances for fetal survival, while protecting against potential pathogens. Recent data show that activation of Toll-like receptor (TLR)2 and 4 by low-molecular weight hyaluronic acid (LMW-HA) in the epidermis induces secretion of the antimicrobial peptide β-defensin 2. In the present work, we show that LMW-HA induces vaginal epithelial cells to release different antimicrobial peptides, via activation of TLR2 and TLR4. Further, we found that LMW-HA favors repair of vaginal epithelial injury, involving TLR2 and TLR4, and independently from its classical receptor CD44. This wound-healing activity of LMW-HA is dependent from an Akt/phosphatidylinositol 3 kinase pathway. Therefore, these findings suggest that the vaginal epithelium is more than a simple physical barrier to protect against invading pathogens: on the contrary, this surface acts as efficient player of innate host defense, which may modulate its antimicrobial properties and injury restitution activity, following LMW-HA stimulation; this activity may furnish an additional protective activity to this body compartment, highly and constantly exposed to microbiota, ameliorating the self-defense of the vaginal epithelium in both basal and pathological conditions. 相似文献
306.
Jay Barlow John Calambokidis Erin A. Falcone C. Scott Baker Alexander M. Burdin Phillip J. Clapham John K. B. Ford Christine M. Gabriele Richard LeDuc David K. Mattila Terrance J. Quinn II Lorenzo Rojas‐Bracho Janice M. Straley Barbara L. Taylor Jorge Urbán R. Paul Wade David Weller Briana H. Witteveen Manami Yamaguchi 《Marine Mammal Science》2011,27(4):793-818
We estimated the abundance of humpback whales in the North Pacific by capture‐recapture methods using over 18,000 fluke identification photographs collected in 2004–2006. Our best estimate of abundance was 21,808 (CV = 0.04). We estimated the biases in this value using a simulation model. Births and deaths, which violate the assumption of a closed population, resulted in a bias of +5.2%, exclusion of calves in samples resulted in a bias of ?10.5%, failure to achieve random geographic sampling resulted in a bias of ?0.4%, and missed matches resulted in a bias of +9.3%. Known sex‐biased sampling favoring males in breeding areas did not add significant bias if both sexes are proportionately sampled in the feeding areas. Our best estimate of abundance was 21,063 after accounting for a net bias of +3.5%. This estimate is likely to be lower than the true abundance due to two additional sources of bias: individual heterogeneity in the probability of being sampled (unquantified) and the likely existence of an unknown and unsampled breeding area (?8.7%). Results confirm that the overall humpback whale population in the North Pacific has continued to increase and is now greater than some prior estimates of prewhaling abundance. 相似文献
307.
Bycatch in artisanal gill nets threatens the vaquita, Phocoena sinus, with extinction. In 2008 the Mexican government announced a conservation action plan for this porpoise, with three options for a protected area closed to gill net fishing. The probability of success of each of the three options was estimated with a Bayesian population model, where success was defined as an increase in vaquita abundance after 10 yr. The model was fitted to data on abundance, bycatch, and fishing effort, although data were sparse and imprecise. Under the first protected area option, the existing Refuge Area for the Protection of the Vaquita, bycatch was about 7% of population size, and probability of success was 0.08. Under the second option with a larger protected area, the probability of success was 0.35. The third option was large enough to eliminate vaquita bycatch and had a probability of success >0.99. Probability of success was reduced if elimination of vaquita bycatch was delayed or incomplete. Despite considerable efforts by the Mexican government to support vaquita conservation, abundance will probably continue to decline unless additional measures to reduce vaquita bycatch are taken, such as banning gill nets within the vaquita's range and developing effective alternative fishing gear. 相似文献
308.
Zarrelli A Sgambato A Petito V De Napoli L Previtera L Di Fabio G 《Bioorganic & medicinal chemistry letters》2011,21(15):4389-4392
Silybin is the major flavonolignan of silymarin and it displays a plethora of biological effects, generally ascribed to its antioxidant properties. Herein we shall describe an efficient synthetic strategy to obtain a variety of new and more water-soluble silybin and 2,3-dehydrosilybin (DHS) derivatives in which the 23-hydroxyl group was converted to a sulfate, phosphodiester, or amine group, using a solution-phase approach. Furthermore a new and efficient method for the preparation of DHS from silybin was developed and optimised.The antioxidant properties of the new compounds were evaluated in a cellular model in vivo and they displayed an antioxidant activity comparable to or higher than silybin and DHS, being able to prevent H2O2-induced generation of intracellular reactive oxygen species (ROS). Most of the derivatives also displayed a better hydrophilicity while retaining the biological activities of silybin and they might broaden the in vivo applications of this class of natural compounds. 相似文献
309.
Nehring V Evison SE Santorelli LA d'Ettorre P Hughes WO 《Proceedings. Biological sciences / The Royal Society》2011,278(1714):1942-1948
Although social groups are characterized by cooperation, they are also often the scene of conflict. In non-clonal systems, the reproductive interests of group members will differ and individuals may benefit by exploiting the cooperative efforts of other group members. However, such selfish behaviour is thought to be rare in one of the classic examples of cooperation--social insect colonies--because the colony-level costs of individual selfishness select against cues that would allow workers to recognize their closest relatives. In accord with this, previous studies of wasps and ants have found little or no kin information in recognition cues. Here, we test the hypothesis that social insects do not have kin-informative recognition cues by investigating the recognition cues and relatedness of workers from four colonies of the ant Acromyrmex octospinosus. Contrary to the theoretical prediction, we show that the cuticular hydrocarbons of ant workers in all four colonies are informative enough to allow full-sisters to be distinguished from half-sisters with a high accuracy. These results contradict the hypothesis of non-heritable recognition cues and suggest that there is more potential for within-colony conflicts in genetically diverse societies than previously thought. 相似文献
310.
Guerrini G Ciciani G Bruni F Selleri S Martini C Daniele S Ghelardini C Di Cesare Mannelli L Costanzo A 《Bioorganic & medicinal chemistry》2011,19(24):7441-7452
The identification of compounds with selective anxiolytic-like effects, exerted through the benzodiazepine site on γ-aminobutyric acid type A (GABAA) receptors, and that show pronounced antihyperalgesia in several pain models, has oriented research towards the development of new agents for the relief of pain. Starting from our previously reported ligands at the benzodiazepine site on GABAA receptors showing selective anxiolytic-like effects, we have designed new compounds with the aim of identifying those devoid of the typical side effects of the classical benzodiazepines. Our preliminary results indicate that compounds 4, 10(±) and 11 have a very promising antihyperalgesic profile in different animal pain models (peripheral mono-neuropathy, STZ-induced hyperalgesia). In particular 11 exhibits high potency since it exerted its protective effect starting from the dose of 3 mg/kg po, after single injection. 相似文献