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排序方式: 共有320条查询结果,搜索用时 46 毫秒
131.
A Novel Biological Role of α‐Mangostin in Modulating Inflammatory Response Through the Activation of SIRT‐1 Signaling Pathway
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132.
133.
Analysis of extracellular vesicles produced in the biofilm by the dimorphic yeast Pichia fermentans
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134.
135.
Lorenza Dalla Costa Ana L. Pinto-Sintra Manuela Campa Valentino Poletti Lucia Martinelli Mickael Malnoy 《Plant Cell, Tissue and Organ Culture》2014,118(3):471-484
T-DNA chimeric integration and unexpected transgene expression are relevant constraints affecting transgenic plants. This study aims to properly investigate the occurrence of these events and to what extent they may be related. The final goal is to develop an effective screening tool for earlier selection of proper transgenic lines. A strategy based on qPCR and Southern blot was adopted for evaluating gus and Egfp chimerism degree in transgenic Vitis vinifera cv ‘Chardonnay’. Of nine transgenic lines, one had a very high chimerism value, which was shown to be associated with minimal transgene expression. The evaluation of the gus gene over time and space on a line selected as a model showed that transgene’s chimerism was stable and uniform throughout plant tissues whilst its expression was highly variable. Transgene chimerism issue was investigated in detail and useful hints were given for selecting the most favorable transgenic plants and for proper planning of in vitro and ex vitro experiments. 相似文献
136.
Nunzia Scotti Fiammetta Alagna Enrico Ferraiolo Gelsomina Formisano Lorenza Sannino Luigi Buonaguro Angelo De Stradis Alessandro Vitale Luigi Monti Stefania Grillo Franco M. Buonaguro Teodoro Cardi 《Planta》2009,229(5):1109-1122
Plants have been recognized as a promising production platform for recombinant pharmaceutical proteins. The human immunodeficiency
virus Gag (Pr55gag) structural polyprotein precursor is a prime candidate for developing a HIV-1 vaccine, but, so far, has been expressed at
very low level in plants. The aim of this study was to investigate factors potentially involved in Pr55gag expression and increase protein yield in plant cells. In transient expression experiments in various subcellular compartments,
the native Pr55gag sequence could be expressed only in the chloroplast. Experiments with truncated subunits suggested a negative role of the
5′-end on the expression of the full gene in the cytosol. Stable transgenic plants were produced in tobacco by Agrobacterium-mediated nuclear transformation with protein targeted to plastids, and biolistic-mediated plastid transformation. Compared
to the nuclear genome, the integration and expression of the gag transgene in the plastome resulted in significantly higher protein accumulation levels (up to 7–8% TSP, equivalent to 312–363 mg/kg
FW). In transplastomic plants, a 25-fold higher protein accumulation was obtained by translationally fusing the Pr55gag polyprotein to the N-terminus of the plastid photosynthetic RbcL protein. In chloroplasts, the Pr55gag polyprotein was processed in a pattern similar to that achieved by the viral protease, the processing being more extended
in older leaves of mature plants. The Gag proteins produced in transgenic plastids were able to assemble into particles resembling
VLPs produced in baculovirus/insect cells and E. coli systems. These results indicate that plastid transformation is a promising tool for HIV antigen manufacturing in plant cells.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
IGV publication no. 330 相似文献
137.
Konstantin Arnold Florian Kiefer Jürgen Kopp James N. D. Battey Michael Podvinec John D. Westbrook Helen M. Berman Lorenza Bordoli Torsten Schwede 《Journal of structural and functional genomics》2009,10(1):1-8
Structural Genomics has been successful in determining the structures of many unique proteins in a high throughput manner.
Still, the number of known protein sequences is much larger than the number of experimentally solved protein structures. Homology
(or comparative) modeling methods make use of experimental protein structures to build models for evolutionary related proteins.
Thereby, experimental structure determination efforts and homology modeling complement each other in the exploration of the
protein structure space. One of the challenges in using model information effectively has been to access all models available
for a specific protein in heterogeneous formats at different sites using various incompatible accession code systems. Often,
structure models for hundreds of proteins can be derived from a given experimentally determined structure, using a variety
of established methods. This has been done by all of the PSI centers, and by various independent modeling groups. The goal
of the Protein Model Portal (PMP) is to provide a single portal which gives access to the various models that can be leveraged
from PSI targets and other experimental protein structures. A single interface allows all existing pre-computed models across
these various sites to be queried simultaneously, and provides links to interactive services for template selection, target-template
alignment, model building, and quality assessment. The current release of the portal consists of 7.6 million model structures
provided by different partner resources (CSMP, JCSG, MCSG, NESG, NYSGXRC, JCMM, ModBase, SWISS-MODEL Repository). The PMP
is available at and from the PSI Structural Genomics Knowledgebase. 相似文献
138.
David Chamorro Lourdes Alarcón Arturo Ponce Rocio Tapia Héctor González-Aguilar Martha Robles-Flores Teresa Mejía-Castillo José Segovia Yamir Bandala Eusebio Juaristi Lorenza González-Mariscal 《Molecular biology of the cell》2009,20(18):4120-4129
Here, we have analyzed the subcellular destiny of newly synthesized tight junction protein zona occludens (ZO)-2. After transfection in sparse cells, 74% of cells exhibit ZO-2 at the nucleus, and after 18 h the value decreases to 17%. The mutation S369A located within the nuclear exportation signal 1 of ZO-2 impairs the nuclear export of the protein. Because Ser369 represents a putative protein kinase C (PKC) phosphorylation site, we tested the effect of PKC inhibition and stimulation on the nuclear export of ZO-2. Our results strongly suggest that the departure of ZO-2 from the nucleus is regulated by phosphorylation at Ser369 by novel PKCε. To test the route taken by ZO-2 from synthesis to the plasma membrane, we devised a novel nuclear microinjection assay in which the nucleus served as a reservoir for anti-ZO-2 antibody. Through this assay, we demonstrate that a significant amount of newly synthesized ZO-2 goes into the nucleus and is later relocated to the plasma membrane. These results constitute novel information for understanding the mechanisms that regulate the intracellular fate of ZO-2. 相似文献
139.
Eid W Steger M El-Shemerly M Ferretti LP Peña-Diaz J König C Valtorta E Sartori AA Ferrari S 《EMBO reports》2010,11(12):962-968
End resection of DNA-which is essential for the repair of DNA double-strand breaks (DSBs) by homologous recombination-relies first on the partnership between MRE11-RAD50-NBS1 (MRN) and CtIP, followed by a processive step involving helicases and exonucleases such as exonuclease 1 (EXO1). In this study, we show that the localization of EXO1 to DSBs depends on both CtIP and MRN. We also establish that CtIP interacts with EXO1 and restrains its exonucleolytic activity in vitro. Finally, we show that on exposure to camptothecin, depletion of EXO1 in CtIP-deficient cells increases the frequency of DNA-PK-dependent radial chromosome formation. Thus, our study identifies new functions of CtIP and EXO1 in DNA end resection and provides new information on the regulation of DSB repair pathways, which is a key factor in the maintenance of genome integrity. 相似文献
140.
Use of space and habitat selection by roe deer Capreolus capreolus in a Mediterranean coastal area: how does woods landscape affect home range? 总被引:2,自引:0,他引:2
Paolo Lamberti Lorenza Mauri Enrico Merli Stefania Dusi Marco Apollonio 《Journal of Ethology》2006,24(2):181-188
The home-range dynamics and habitat selection of nine roe deer were studied from March 1994 to August 1994 in the Maremma Natural Park along the Tyrrhenian coast of Italy. The habitat was highly fragmented, with open agricultural fields prevailing in the study area (57%); the climate was Mediterranean. Data on spatial behaviour were collected by radio-tracking techniques. Habitat selection and structure were investigated by compositional and landscape analysis, both within the study area and within the home ranges. Animals of our sample showed spatial-use patterns varying from stationary to roaming. Stationary individuals used small home ranges while roaming ones moved, especially during the reproductive period in July and August. The percentage and structure of woodlands influenced the size of home ranges and the behaviour of males: stationary males used large amounts of woodlands within their home ranges and showed a territorial behaviour whereas males that used a high percentage of fields showed wider home ranges even during the territorial period. Females seemed to be less influenced by the presence and patch-structure of woodland within their home range. Landscape structure and habitat composition seemed to be important factors influencing the spatial behaviour of this roe deer population. 相似文献