全文获取类型
收费全文 | 1288篇 |
免费 | 84篇 |
国内免费 | 1篇 |
出版年
2023年 | 8篇 |
2022年 | 23篇 |
2021年 | 42篇 |
2020年 | 30篇 |
2019年 | 41篇 |
2018年 | 48篇 |
2017年 | 34篇 |
2016年 | 58篇 |
2015年 | 65篇 |
2014年 | 94篇 |
2013年 | 102篇 |
2012年 | 151篇 |
2011年 | 111篇 |
2010年 | 76篇 |
2009年 | 55篇 |
2008年 | 65篇 |
2007年 | 79篇 |
2006年 | 58篇 |
2005年 | 60篇 |
2004年 | 45篇 |
2003年 | 46篇 |
2002年 | 31篇 |
2001年 | 10篇 |
2000年 | 5篇 |
1999年 | 4篇 |
1998年 | 6篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 4篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1984年 | 1篇 |
1982年 | 2篇 |
1970年 | 1篇 |
1968年 | 1篇 |
1919年 | 1篇 |
排序方式: 共有1373条查询结果,搜索用时 15 毫秒
191.
Cristóbal Espinosa Ruiz Lorena Cabrera José Ángel López-Jiménez Salvador Zamora Francisca Pérez-Llamas 《Journal of physiology and biochemistry》2018,74(1):171-177
The infusion tea extracted from the leaves of the plant Camellia sinensis can be used in the prevention of cancer, cardiovascular and neurodegenerative diseases, and aging, while adriamycin (ADR) is an anticancer drug that increases oxidative stress in cells. The present study evaluated the protective effect of the long-term consumption of white tea used at two different doses against the oxidative stress produced by aging and acute oxidation caused ADR treatment. At wearing, rats received distilled water (control), or 0.15 (dose 1) or 0.45 mg (dose 2) of solid tea extract/kilogram body weight in their drink. At 12 months, about half of the rats of each group were injected with a bolus of ADR, and six rats of the control group with an injection of saline solution and sacrificed. The rest of the animals continued in their cages until 24 months of age, when they were sacrificed. Lipid and protein oxidation of liver and brain microsomes was analyzed by measuring hydroperoxide and carbonyl levels. White tea consumption for 12 months at a non-pharmacological dose was seen to reverse the oxidative damage caused by ADR in both liver and brain, while the consumption of white tea for 20 months at a non-pharmacological dose had no effect on carbonyl or hydroperoxides in these tissues. The long-term ingestion of white tea protected tissues from acute oxidative stress but did not affect chronic oxidative agents such aging. 相似文献
192.
L‐asparaginase isolated from Streptomyces ansochromogenes promotes Th1 profile and activates CD8+ T cells in human PBMC: an in vitro investigation
下载免费PDF全文
![点击此处可从《Journal of applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
193.
Soraia Machado Cordeiro Cristiane Wanderley Cardoso Lorena Galvão de Araújo Luis Eduardo Ribeiro Jailton Azevedo Rita de Cassia Vilasboas Silva Mitermayer Galvão dos Reis Albert Icksang Ko Joice Neves Reis 《Microbes and infection / Institut Pasteur》2018,20(1):19-24
Invasive meningococcal disease (IMD) is a major public health problem worldwide. An epidemic of serogroup C (NmC) IMD occurred in 2010 in the city of Salvador. In this study, we describe the antigenic and genetic characterization of meningococcal isolates collected from meningitis cases in Salvador from 2001 to 2012. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were performed for the analysis of IMD isolates. A total of 733 cases were identified, and the serogroup was determined for 391 (53.0%) of these. Most cases were caused by NmC (53%) or B (47%). The most prevalent strains were B:4,7:P1.19,15 (32.9%; 129/391) and C:23:P1.14–6 (28.6%; 112/391). Based on PFGE/MLST analysis, 71.3% (77/108 PFGE-tested isolates) clustered as two clones of sequence type ST-3779 and ST-3780, both belonging to the ST-103 clonal complex. ST-3779 has been detected in Salvador since 1996 and together with ST-3780 became predominant after 2005. There was a predominance of C:23:P1.14–6, ST-3779/3780 in Salvador during the period of 2007–2012, establishing a major clonal lineage, which remained in the community for a long time; this has serious implications for public health, particularly in terms of prevention and control strategies of IMD. 相似文献
194.
Lorena M. Breed 《The Western journal of medicine》1919,17(10):371-373
195.
Lorena Carro Valeria Razmilic Imen Nouioui Lee Richardson Che Pan Patrycja Golinska Juan A. Asenjo Alan T. Bull Hans-Peter Klenk Michael Goodfellow 《Antonie van Leeuwenhoek》2018,111(8):1375-1387
Innovative procedures were used to selectively isolate small numbers of Micromonospora strains from extreme hyper-arid and high altitude Atacama Desert soils. Micromonosporae were recognised on isolation plates by their ability to produce filamentous microcolonies that were strongly attached to the agar. Most of the isolates formed characteristic orange colonies that lacked aerial hyphae and turned black on spore formation, whereas those from the high altitude soil were dry, blue-green and covered by white aerial hyphae. The isolates were assigned to seven multi- and eleven single-membered groups based on BOX-PCR profiles. Representatives of the groups were assigned to either multi-membered clades that also contained marker strains or formed distinct phyletic lines in the Micromonospora 16S rRNA gene tree; many of the isolates were considered to be putatively novel species of Micromonospora. Most of the isolates from the high altitude soils showed activity against wild type strains of Bacillus subtilis and Pseudomonas fluorescens while those from the rhizosphere of Parastrephia quadrangulares and from the Lomas Bayas hyper-arid soil showed resistance to UV radiation. 相似文献
196.
197.
Laura Tatjer Almudena Sacristán-Reviriego Carlos Casado Asier González Boris Rodríguez-Porrata Lorena Palacios David Canadell Albert Serra-Cardona Humberto Martín María Molina Joaquín Ari?o 《Genetics》2016,202(1):141-156
The Saccharomyces cerevisiae type 2C protein phosphatase Ptc1 is required for a wide variety of cellular functions, although only a few cellular targets have been identified. A genetic screen in search of mutations in protein kinase–encoding genes able to suppress multiple phenotypic traits caused by the ptc1 deletion yielded a single gene, MKK1, coding for a MAPK kinase (MAPKK) known to activate the cell-wall integrity (CWI) Slt2 MAPK. In contrast, mutation of the MKK1 paralog, MKK2, had a less significant effect. Deletion of MKK1 abolished the increased phosphorylation of Slt2 induced by the absence of Ptc1 both under basal and CWI pathway stimulatory conditions. We demonstrate that Ptc1 acts at the level of the MAPKKs of the CWI pathway, but only the Mkk1 kinase activity is essential for ptc1 mutants to display high Slt2 activation. We also show that Ptc1 is able to dephosphorylate Mkk1
in vitro. Our results reveal the preeminent role of Mkk1 in signaling through the CWI pathway and strongly suggest that hyperactivation of Slt2 caused by upregulation of Mkk1 is at the basis of most of the phenotypic defects associated with lack of Ptc1 function. 相似文献
198.
199.
Rhizobacterial Community Structures Associated with Native Plants Grown in Chilean Extreme Environments 总被引:1,自引:0,他引:1
200.