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11.
Procedures for the synthesis of fluorescent and radiolabeled analogues of phosphatidic acid are described. The fluorophore 7-nitrobenzo-2-oxa-1,3-diazole (NBD) was coupled to 6-amino-caproic acid and 12-aminododecanoic acid by reaction of NBD-chloride with the amino acids under mild alkaline conditions at room temperature. 1,2-Dioleoyl-sn-[U-14C]glycerol 3-phosphate was prepared by acylation of sn-[U-14C]glycerol 3-phosphate with oleic acid anhydride using dimethylaminopyridine as the catalyst. This compound was converted to 1-oleoyl-sn-[U-14C]glycerol 3-phosphate by hydrolysis with phospholipase A2. The lysophosphatidic acid was reacylated with NBD-aminocaproyl imidazole or NBD-aminododecanoyl imidazole to form the fluorescent, radiolabeled analogue of phosphatidic acid. Fluorescent, non-radiolabeled analogues of phosphatidic acid were prepared by phospholipase D hydrolysis of fluorescent phosphatidylcholine. 相似文献
12.
Our currently developed fluorescence video microscope can measure fluorescence intensities with an error of ±1.5% of full scale in 65 536 different positions of a microscope field. With a video frame freeze acquisition time of 33 ms, time-dependent changes of this order of time or slower can be followed. Using cells which have absorbed pyrene-1-butyrate to an intracellular concentration of 0.05 to 1 mM, the changes in fluorescence intensity with oxygen concentration are easily measured. The spatial resolution for data collection is 0.5 μm when a 54X objective is used. The individual Stern-Volmer quenching constants of each individual pixel were measured for agar slices and mouse liver cells treated with pyrenebutyric acid. The distribution of quenching constants for agar follows a normal curve about a mean value of 16 · 10?4 torr?1. The data for mouse liver cells gave a non-normal distribution of quenching constants with a mean value of 18 · 10?4 torr?1. The greater spread of the data from cells is interpreted as evidence for a real biological variation in the solubility coefficent of oxygen in different locations within the cell. In all the cells examined, this distribution has been observed to be non-random and appears to be associated with specific cell structures. 相似文献
13.
Kenneth J. Longmuir Jeanne M. Snyder Carole R. Mendelson John M. Johnston 《Archives of biochemistry and biophysics》1981,212(2):491-500
Lung tissue obtained from fetal rabbits of 23 days gestational age was maintained in organ culture to study the in vitro formation of lamellar body phospholipids. During the culture period, the epithelium of the prealveolar ducts of the explants differentiated to form type II pneumonocytes. After 8 days in culture, the explants were harvested, homogenized, and two lamellar body fractions were isolated by sucrose density gradient centrifugation. The lamellar body fraction which best retained the distinct multilamellar structure was recovered at the interface between a solution of buffer without sucrose and buffer containing 0.41 m sucrose. The phospholipid compositions of both lamellar body fractions were similar to those reported for lamellar bodies and surfactant isolated from fetal rabbit lung, with the exception of a slightly higher phosphatidylethanolamine content. The disaturated phosphatidylcholine content of the lamellar body fractions, expressed as a percentage of total lipid phosphorus, was not influenced by the presence of palmitate in the medium. 相似文献
14.
Tissue adaptation to oxygen lack 总被引:1,自引:0,他引:1
15.
A recently published equation for oxygen uptake in cells (Hills, 1970) is corrected and applied to data gathered under appropriate
experimental conditions. The result is found to support the hypothesis of facilitated intracellular oxygen transport. 相似文献
16.
Background
Adverse drug reactions (ADRs) are now recognized as an important cause of hospital admissions, with a proportion ranging from 0.9–7.9%. They also constitute a significant economic burden. We thus aimed at determining the prevalence and the economic burden of ADRs presenting to Medical Emergency Department (ED) of a tertiary referral center in India 相似文献17.
Janie L. Baratta Anthony Ngo Bryan Lopez Natasha Kasabwalla Kenneth J. Longmuir Richard T. Robertson 《Histochemistry and cell biology》2009,131(6):713-726
The cellular organization of normal mouse liver was studied using light and electron microscopy and quantitative immunocytochemical
techniques. The general histological organization of the mouse liver is similar to livers of other mammalian species, with
a lobular organization based on the distributions of portal areas and central venules. The parenchymal hepatocytes were detected
with immunocytochemical techniques to recognize albumin or biotin containing cells. The macrophage Kupffer cells were identified
with F4-80 immunocytochemistry, Ito stellate cells were identified with GFAP immunocytochemistry, and endothelial cells were
labeled with the CD-34 antibody. Kupffer cells were labeled with intravascularly administered fluorescently labeled latex
microspheres of both large (0.5 μm) and small (0.03 μm) diameters, while endothelial cells were labeled only with small diameter
microspheres. Neither hepatocytes nor Ito stellate cells were labeled by intravascularly administered latex microspheres.
The principal fine structural features of hepatocytes and non-parenchymal cells of mouse liver are similar to those reported
for rat. Counts of immunocytochemically labeled cells with stained nuclei indicated that hepatocytes constituted approximately
52% of all labeled cells, Kupffer cells about 18%, Ito cells about 8%, and endothelial cells about 22% of all labeled cells.
Approximately, 35% of the hepatocytes contained two nuclei; none of the Kupffer or Ito cells were double nucleated. The presence
of canaliculi and a bile duct system appear similar to that reported for other species. The cellular organization of the mouse
liver is quite similar to that of other mammalian species, confirming that the mouse presents a useful animal model for studies
of liver structure and function. 相似文献
18.
19.
Block of outward current in cardiac purkinje fibers by injection of quaternary ammonium ions 总被引:6,自引:5,他引:1
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We have studied the effects of iontophoretic injection of the quaternary ammonium compounds tetraethylammonium (TEA) and tetrabutylammonium (TBA) in cardiac purkinje fibers. We find that TBA(+) is a more effective blocker than TEA(+), but injection of either compound reduces the time-dependent outward plateau currents, transient outward current (I(to)), and the delayed rectifier (I(x)). Our findings provide evidence that these outward cardiac currents are carried by channels that in some respects are pharmacologically similar to squid axon potassium channels. We demonstrate that this procedure is a new tool that can be useful in the analysis of membrane currents in the heart. 相似文献
20.
The first Australian record of the lily thrips, Liothrips vaneeckei Priesner, is reported from a bulb farm in Warragul South, Victoria. It is an occasional pest of Lilium bulbs, both in the field and in storage, particularly in the USA and several European countries, and is also infrequently found in considerable numbers on the corms of orchids. 相似文献