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61.
Relationship between the Geotropic Response and the Evolution of the Statenchyma in Roots of Asparagus officinalis. The evolution of the statenchyma in roots of Asparagus of ficinalis seedlings, grown in obscurity, was followed during the first 17 days. After 7 days of etiolation, a decrease of both the average diameter of the amyloplasts and the average number of these organelles was observed in the central root cap cells. If the seedlings were illuminated (with white light) from the 7th day, the average number of statoliths increased rapidly in the statocytes. The volume of these organelles undergoes the same variation in etiolated and in illuminated plants. The initial rate of curvature (Vi) of the roots (stimulated in a horizontal position) and the volume of amyloplasts (Vac) in their caps were analysed as a function of the time of germination in obscurity (from the 8th to the 17th day). It was found that Vi increased as a linear function of the logarithm of Vac, which confirms that the weight of the amyloplasts of the statocytes may play a role in the geotropic stimulation of the roots. 相似文献
62.
JEAN-FRANÇOIS RIOU DE-LI SHI THIERRY DARRIBÈRE JEAN-CLAUDE BOUCAUT JACQUES CHARLEMAGNE 《Development, growth & differentiation》1987,29(5):443-454
We have previously reported the identification of cell surface glycoproteins in Pleurodeles waltlii gastrulae. In an attempt to study the expression of three of these cell surface glycoproteins (proteins referred to 1, 11 and 14), we have produced monoclonal and polyclonal antibodies by immunizing mice with the spots of the three selected glycoproteins excised from 2D-gels. Expression of the three glycoproteins was detected on the surfaces of all cells during embryonic development. Before hatching, proteins 1, 11 and 14 become expressed in a limited number of tissues. 相似文献
63.
Flint J Nurizzo D Harding SE Longman E Davies GJ Gilbert HJ Bolam DN 《Journal of molecular biology》2004,337(2):417-426
The structural and thermodynamic basis for carbohydrate-protein recognition is of considerable importance. NCP-1, which is a component of the Piromyces equi cellulase/hemicellulase complex, presents a provocative model for analyzing how structural and mutational changes can influence the ligand specificity of carbohydrate-binding proteins. NCP-1 contains two "family 29" carbohydrate-binding modules designated CBM29-1 and CBM29-2, respectively, that display unusually broad specificity; the proteins interact weakly with xylan, exhibit moderate affinity for cellulose and mannan, and bind tightly to the beta-1,4-linked glucose-mannose heteropolymer glucomannan. The crystal structure of CBM29-2 in complex with cellohexaose and mannohexaose identified key residues involved in ligand recognition. By exploiting this structural information and the broad specificity of CBM29-2, we have used this protein as a template to explore the evolutionary mechanisms that can lead to significant changes in ligand specificity. Here, we report the properties of the E78R mutant of CBM29-2, which displays ligand specificity that is different from that of wild-type CBM29-2; the protein retains significant affinity for cellulose but does not bind to mannan or glucomannan. Significantly, E78R exhibits a stoichiometry of 0.5 when binding to cellohexaose, and both calorimetry and ultracentrifugation show that the mutant protein displays ligand-mediated dimerization in solution. The three-dimensional structure of E78R in complex with cellohexaose reveals the intriguing molecular basis for this "dimeric" binding mode that involves the lamination of the oligosaccharide between two CBM molecules. The 2-fold screw axis of the ligand is mirrored in the orientation of the two protein domains with adjacent sugar rings stacking against the equivalent aromatic residues in the binding site of each protein molecule of the molecular sandwich. The sandwiching of an oligosaccharide chain between two protein modules, leading to ligand-induced formation of the binding site, represents a completely novel mechanism for protein-carbohydrate recognition that may mimic that displayed by naturally dimeric protein-carbohydrate interactions. 相似文献
64.
Objective: This study was undertaken to assess the presence and degree of anxiety and depression in a group of UK patients with primary Sjögren's syndrome (1°SS). Design: Cross‐sectional. Setting: Department of Oral Medicine, Liverpool University Dental Hospital. Subjects: Eighty adult patients; 40 diagnosed with 1°SS according to the revised European Criteria and 40 age/gender‐matched controls with no history of chronic illness. Intervention: Hospital Anxiety and Depression Scale (HADS), a self‐administered questionnaire designed to evaluate the presence and degree of anxiety and depression in a clinical setting. Main outcome measures: Age, gender, Hospital Anxiety and Depression Scale (HADS). Results: Forty patients with 1°SS and 40/age/gender‐matched controls completed the HADS. Scores for anxiety in both the 1°SS and control groups showed no statistically significant difference. Patients with 1°SS had statistically significant higher, mean HADS scores for depression than the controls. There was an increased prevalence of ‘definite’ clinical depression in the 1°SS group. Conclusion: Patients with 1°SS appear to be at increased risk from clinical depression. Early recognition and appropriate intervention is therefore essential to reduce the negative impact of depression on the patient's quality of life and outcome of their disease. 相似文献
65.
Sellick GS Longman C Brockington M Mahjneh I Sagi L Bushby K Topaloğlu H Muntoni F Houlston RS 《Human genetics》2005,117(2-3):207-212
The congenital muscular dystrophies (CMD) are a heterogeneous group of autosomal recessive disorders, which present within the first 6 months of life with hypotonia, muscle weakness and contractures, associated with dystrophic changes on skeletal muscle biopsy. We have previously reported a large consanguineous family segregating merosin-positive congenital muscular dystrophy, in which involvement of known CMD loci was excluded. A genome-wide linkage search of the family conducted using microsatellite markers spaced at 10-Mb intervals failed to identify a disease locus. A second scan using a high-density SNP array, however, permitted a novel CMD locus on 4p16.3 to be identified (multipoint LOD score 3.4). Four additional consanguineous CMD families with a similar phenotype were evaluated for linkage to a 4.14-Mb interval on 4p16.3; however, none showed any evidence of linkage to the region. Our findings further illustrate the utility of highly informative SNP arrays compared with standard panels of microsatellite markers for the mapping of recessive disease loci. 相似文献
66.
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68.
STÉPHANIE DEPICKÈRE G. MARCELO RAMÍREZ ÁVILA DOMINIQUE FRESNEAU JEAN-LOUIS DENEUBOURG 《Ecological Entomology》2008,33(2):225-231
Abstract. 1. Aggregation of individuals, a basic behaviour in social species, plays an essential role in many aspects of animal life (reproduction, defence, and alimentation). Understanding how this phenomenon is modulated is important to comprehend the social organisation of the group.
2. In social insects, aggregation is influenced by environmental (e.g. the light level) and social (e.g. polyethism in monomorphic ants) factors. Ants display a great variation of biological characteristics (e.g. queen number, polymorphism, division of labour, etc.) that are likely to influence the level of inter-attraction and so the aggregation.
3. The present research focused on one biological characteristic: the morphological castes (minors, majors), testing the hypothesis that minors will aggregate more than majors due to their greater need to fight against the loss of heat and to increase their self-protection.
4. Aggregation experiments were conducted on two highly polymorphic species, Atta sexdens rubropilosa and Solenopsis interrupta , using the two extreme morphological castes (majors and minors).
5. All castes exhibited a low level of aggregation: 40–50% of workers assembled for both species, the biggest cluster involving 20% of the total population. The lack of difference between morphological castes in the aggregation shows the weak influence of polymorphism on the interactions between ants.
6. It is concluded that the main factor modulating the aggregation behaviour is polyethism, i.e. the division of labour associated with the presence of an outside-the-nest experience: workers that only take care of the brood, without outside world experience (brood-tenders) assembling more than foraging workers (foragers). 相似文献
2. In social insects, aggregation is influenced by environmental (e.g. the light level) and social (e.g. polyethism in monomorphic ants) factors. Ants display a great variation of biological characteristics (e.g. queen number, polymorphism, division of labour, etc.) that are likely to influence the level of inter-attraction and so the aggregation.
3. The present research focused on one biological characteristic: the morphological castes (minors, majors), testing the hypothesis that minors will aggregate more than majors due to their greater need to fight against the loss of heat and to increase their self-protection.
4. Aggregation experiments were conducted on two highly polymorphic species, Atta sexdens rubropilosa and Solenopsis interrupta , using the two extreme morphological castes (majors and minors).
5. All castes exhibited a low level of aggregation: 40–50% of workers assembled for both species, the biggest cluster involving 20% of the total population. The lack of difference between morphological castes in the aggregation shows the weak influence of polymorphism on the interactions between ants.
6. It is concluded that the main factor modulating the aggregation behaviour is polyethism, i.e. the division of labour associated with the presence of an outside-the-nest experience: workers that only take care of the brood, without outside world experience (brood-tenders) assembling more than foraging workers (foragers). 相似文献
69.
Abstract. 1. Sexual conflict can play an important role in the evolution of animal life-history characteristics, including lifespan. Seaweed flies show an increase in mortality rates when exposed to brown algae. The seaweed stimulates females to oviposit and males to mount females. Females typically respond to male mounts by performing a violent rejection response.
2. Here the contribution of sexual conflict to the increase in mortality seen in the presence of seaweed was determined. The survival of single and mixed sex pairs of flies was followed in the presence and absence of seaweed.
3. The two sexes showed differential survival rates, with females living longer in the absence of seaweed. The presence of seaweed reduced survival in both sexes. In the presence of seaweed, female survival was lower when paired with a male. Over 40% of the reduction in survival in females in the presence of seaweed appears to be attributable to sexual conflict.
4. The presence of a female did not significantly affect male survival. Thus the mortality cost of being in the presence of the opposite sex and seaweed appears highly asymmetric.
5. In the presence of seaweed, female survival was lower when females were paired with small males. Small males exhibit higher levels of harassment of females, thus it is argued that pre-copulatory sexual conflict is the probable cause of the increased mortality cost to females of being in the presence of both males and seaweed. 相似文献
2. Here the contribution of sexual conflict to the increase in mortality seen in the presence of seaweed was determined. The survival of single and mixed sex pairs of flies was followed in the presence and absence of seaweed.
3. The two sexes showed differential survival rates, with females living longer in the absence of seaweed. The presence of seaweed reduced survival in both sexes. In the presence of seaweed, female survival was lower when paired with a male. Over 40% of the reduction in survival in females in the presence of seaweed appears to be attributable to sexual conflict.
4. The presence of a female did not significantly affect male survival. Thus the mortality cost of being in the presence of the opposite sex and seaweed appears highly asymmetric.
5. In the presence of seaweed, female survival was lower when females were paired with small males. Small males exhibit higher levels of harassment of females, thus it is argued that pre-copulatory sexual conflict is the probable cause of the increased mortality cost to females of being in the presence of both males and seaweed. 相似文献
70.
Shelley D. Crausbay Abby G. Frazier Thomas W. Giambelluca Ryan J. Longman Sara C. Hotchkiss 《Oecologia》2014,175(1):273-284
Growing evidence suggests short-duration climate events may drive community structure and composition more directly than long-term climate means, particularly at ecotones where taxa are close to their physiological limits. Here we use an empirical habitat model to evaluate the role of microclimate during a strong El Niño in structuring a tropical montane cloud forest’s upper limit and composition in Hawai‘i. We interpolate climate surfaces, derived from a high-density network of climate stations, to permanent vegetation plots. Climatic predictor variables include (1) total rainfall, (2) mean relative humidity, and (3) mean temperature representing non-El Niño periods and a strong El Niño drought. Habitat models explained species composition within the cloud forest with non-El Niño rainfall; however, the ecotone at the cloud forest’s upper limit was modeled with relative humidity during a strong El Niño drought and secondarily with non-El Niño rainfall. This forest ecotone may be particularly responsive to strong, short-duration climate variability because taxa here, particularly the isohydric dominant Metrosideros polymorpha, are near their physiological limits. Overall, this study demonstrates moisture’s overarching influence on a tropical montane ecosystem, and suggests that short-term climate events affecting moisture status are particularly relevant at tropical ecotones. This study further suggests that predicting the consequences of climate change here, and perhaps in other tropical montane settings, will rely on the skill and certainty around future climate models of regional rainfall, relative humidity, and El Niño. 相似文献