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21.
Background
Zerumbone is a cytotoxic component isolated from Zingiber zerumbet Smith, a herbal plant which is also known as lempoyang. This new anticancer bioactive compound from Z. zerumbet was investigated for its activity and mechanism in human liver cancer cell lines. 相似文献22.
23.
Moise Bendayan Irene Londono Eugene Paransky 《The journal of histochemistry and cytochemistry》2011,59(10):899-907
Low-voltage (5-kV) transmission electron microscopy revealed a novel aspect of the pancreatic acinar cell secretory granules not previously detected by conventional (80-kV) transmission electron microscopy. Examination of ultra-thin (30-nm) sections of non-osmicated, stain-free pancreatic tissue sections by low-voltage electron microscopy revealed the existence of granules with non-homogeneous matrix and sub-compartments having circular or oval profiles of different electron densities and sizes. Such partition is completely masked when observing tissues after postfixation with osmium tetroxide by low-voltage transmission electron microscopy at 5 kV and/or when thicker sections (70 nm) are examined at 80 kV. This morphological partition reflects an internal compartmentalization of the granule content that was previously predicted by morphological, physiological, and biochemical means. It corresponds to the segregation of the different secretory proteins inside the granule as demonstrated by high-resolution immunocytochemistry and reflects a well-organized aggregation of the secretory proteins at the time of granule formation in the trans-Golgi. Such partition of the granule matrix undergoes changes under experimental conditions known to alter the secretory process such as stimulation of secretion or diabetes. 相似文献
24.
Douglas?Londono Steven?Buyske Stephen?J?Finch Swarkar?Sharma Carol?A?Wise Derek?GordonEmail author 《BMC bioinformatics》2012,13(1):13
Background
Locus heterogeneity is one of the most documented phenomena in genetics. To date, relatively little work had been done on the development of methods to address locus heterogeneity in genetic association analysis. Motivated by Zhou and Pan's work, we present a mixture model of linked and unlinked trios and develop a statistical method to estimate the probability that a heterozygous parent transmits the disease allele at a di-allelic locus, and the probability that any trio is in the linked group. The purpose here is the development of a test that extends the classic transmission disequilibrium test (TDT) to one that accounts for locus heterogeneity. 相似文献25.
Sirois I Groleau J Pallet N Brassard N Hamelin K Londono I Pshezhetsky AV Bendayan M Hébert MJ 《Autophagy》2012,8(6):927-937
The endothelium plays a central role in the regulation of vascular wall cellularity and tone by secreting an array of mediators of importance in intercellular communication. Nutrient deprivation of human endothelial cells (EC) evokes unconventional forms of secretion leading to the release of nanovesicles distinct from apoptotic bodies and bearing markers of multivesicular bodies (MVB). Nutrient deficiency is also a potent inducer of autophagy and vesicular transport pathways can be assisted by autophagy. Nutrient deficiency induced a significant and rapid increase in autophagic features, as imaged by electron microscopy and immunoblotting analysis of LC3-II/LC3-I ratios. Increased autophagic flux was confirmed by exposing serum-starved cells to bafilomycin A 1. Induction of autophagy was followed by indices of an apoptotic response, as assessed by microscopy and poly (ADP-ribose) polymerase cleavage in absence of cell membrane permeabilization indicative of necrosis. Pan-caspase inhibition with ZVAD-FMK did not prevent the development of autophagy but negatively impacted autophagic vacuole (AV) maturation. Adopting a multidimensional proteomics approach with validation by immunoblotting, we determined that nutrient-deprived EC released AV components (LC3I, LC3-II, ATG16L1 and LAMP2) whereas pan-caspase inhibition with ZVAD-FMK blocked AV release. Similarly, nutrient deprivation in aortic murine EC isolated from CASP3/caspase 3-deficient mice induced an autophagic response in absence of apoptosis and failed to prompt LC3 release. Collectively, the present results demonstrate the release of autophagic components by nutrient-deprived apoptotic human cells in absence of cell membrane permeabilization. These results also identify caspase-3 as a novel regulator of AV release. 相似文献
26.
VanEpps JS Londono R Nieponice A Vorp DA 《Biomechanics and modeling in mechanobiology》2009,8(1):57-66
Cyclic flexure of the coronary arteries can lead to spatially varying fluid and solid stress patterns. These patterns may
explain the heterogenous distribution of atherosclerotic lesions. Here we describe the design and validation of an experimental
system to simulate coronary-like flexure dynamics on intact arterial segments ex vivo. Our previously described ex vivo perfusion
system was modified with a polymer flexure membrane controlled by a custom data acquisition/motion control system. The system
was validated by perfusing arterial segments with pulsatile hemodynamics with or without cyclic flexure. Digital images were
obtained to quantify dynamic vessel curvature and arc length. Tissue integrity was assessed by histology. The device generated
physiologic curvatures (0–1.8 cm−1) at 1 Hz with a physiologic phase relationship with the pressure waveform. Additionally, the in vivo longitudinal extension
ratio (40%) was maintained within 2.3% during the flexure cycle. Twelve hours of cyclic contact with the membrane did not
compromise arterial segment integrity. This device provides a novel method to examine how the local biomechanical milieu could
impact atherosclerotic lesion localization. 相似文献
27.
Molecular phylogenetics reveal multiple tertiary vicariance origins of the African rain forest trees
Background
Tropical rain forests are the most diverse terrestrial ecosystems on the planet. How this diversity evolved remains largely unexplained. In Africa, rain forests are situated in two geographically isolated regions: the West-Central Guineo-Congolian region and the coastal and montane regions of East Africa. These regions have strong floristic affinities with each other, suggesting a former connection via an Eocene pan-African rain forest. High levels of endemism observed in both regions have been hypothesized to be the result of either 1) a single break-up followed by a long isolation or 2) multiple fragmentation and reconnection since the Oligocene. To test these hypotheses the evolutionary history of endemic taxa within a rain forest restricted African lineage of the plant family Annonaceae was studied. Molecular phylogenies and divergence dates were estimated using a Bayesian relaxed uncorrelated molecular clock assumption accounting for both calibration and phylogenetic uncertainties. 相似文献28.
29.
Thomas Dugé de Bernonville Emilien Foureau Claire Parage Arnaud Lanoue Marc Clastre Monica Arias Londono Audrey Oudin Benjamin Houillé Nicolas Papon Sébastien Besseau Ga?lle Glévarec Lucia Atehortùa Nathalie Giglioli-Guivarc’h Benoit St-Pierre Vincenzo De Luca Sarah E. O’Connor Vincent Courdavault 《BMC genomics》2015,16(1)