全文获取类型
收费全文 | 1771篇 |
免费 | 229篇 |
国内免费 | 6篇 |
出版年
2022年 | 18篇 |
2021年 | 26篇 |
2020年 | 8篇 |
2019年 | 13篇 |
2018年 | 22篇 |
2017年 | 13篇 |
2016年 | 36篇 |
2015年 | 78篇 |
2014年 | 71篇 |
2013年 | 79篇 |
2012年 | 113篇 |
2011年 | 105篇 |
2010年 | 66篇 |
2009年 | 61篇 |
2008年 | 87篇 |
2007年 | 92篇 |
2006年 | 91篇 |
2005年 | 77篇 |
2004年 | 78篇 |
2003年 | 59篇 |
2002年 | 55篇 |
2001年 | 64篇 |
2000年 | 54篇 |
1999年 | 76篇 |
1998年 | 28篇 |
1997年 | 27篇 |
1996年 | 20篇 |
1995年 | 20篇 |
1994年 | 14篇 |
1993年 | 18篇 |
1992年 | 50篇 |
1991年 | 37篇 |
1990年 | 39篇 |
1989年 | 28篇 |
1988年 | 36篇 |
1987年 | 28篇 |
1986年 | 26篇 |
1985年 | 19篇 |
1984年 | 16篇 |
1983年 | 12篇 |
1982年 | 11篇 |
1981年 | 10篇 |
1980年 | 11篇 |
1979年 | 12篇 |
1977年 | 12篇 |
1976年 | 9篇 |
1975年 | 10篇 |
1974年 | 10篇 |
1973年 | 11篇 |
1972年 | 10篇 |
排序方式: 共有2006条查询结果,搜索用时 15 毫秒
191.
192.
193.
194.
Mark Barnes Gerrit van Rensburg Wai-Ming Li Kashif Mehmood Sebastian Mackedenski Ching-Man Chan Dustin T. King Andrew L. Miller Chow H. Lee 《The Journal of biological chemistry》2015,290(1):625-639
The ability of its four heterogeneous nuclear RNP-K-homology (KH) domains to physically associate with oncogenic mRNAs is a major criterion for the function of the coding region determinant-binding protein (CRD-BP). However, the particular RNA-binding role of each of the KH domains remains largely unresolved. Here, we mutated the first glycine to an aspartate in the universally conserved GXXG motif of the KH domain as an approach to investigate their role. Our results show that mutation of a single GXXG motif generally had no effect on binding, but the mutation in any two KH domains, with the exception of the combination of KH3 and KH4 domains, completely abrogated RNA binding in vitro and significantly retarded granule formation in zebrafish embryos, suggesting that any combination of at least two KH domains cooperate in tandem to bind RNA efficiently. Interestingly, we found that any single point mutation in one of the four KH domains significantly impacted CRD-BP binding to mRNAs in HeLa cells, suggesting that the dynamics of the CRD-BP-mRNA interaction vary over time in vivo. Furthermore, our results suggest that different mRNAs bind preferentially to distinct CRD-BP KH domains. The novel insights revealed in this study have important implications on the understanding of the oncogenic mechanism of CRD-BP as well as in the future design of inhibitors against CRD-BP function. 相似文献
195.
196.
An Yun Guo Kwok Sui Leung Parco Ming Fai Siu Jiang Hui Qin Simon Kwoon Ho Chow Ling Qin Chi Yu Li Wing Hoi Cheung 《Experimental Animals》2015,64(4):425-433
Sarcopenia is an age-related systemic syndrome with progressive deterioration in skeletal
muscle functions and loss in mass. Although the senescence-accelerated mouse P8 (SAMP8)
was reported valid for muscular ageing research, there was no report on the details such
as sarcopenia onset time. Therefore, this study was to investigate the change of muscle
mass, structure and functions during the development of sarcopenia. Besides the average
life span, muscle mass, structural and functional measurements were also studied. Male
SAMP8 animals were examined at month 6, 7, 8, 9, and 10, in which the right gastrocnemius
was isolated and tested for ex vivo contractile properties and fatigability while the
contralateral one was harvested for muscle fiber cross-sectional area (FCSA) and typing
assessments. Results showed that the peak of muscle mass appeared at month 7 and the onset
of contractility decline was observed from month 8. Compared with month 8, most of the
functional parameters at month 10 decreased significantly. Structurally, muscle fiber type
IIA made up the largest proportion of the gastrocnemius, and the fiber size was found to
peak at month 8. Based on the altered muscle mass, structural and functional outcomes, it
was concluded that the onset of sarcopenia in SAMP8 animals was at month 8. SAMP8 animals
at month 8 should be at pre-sarcopenia stage while month 10 at sarcopenia stage. It is
confirmed that SAMP8 mouse can be used in sarcopenia research with established time line
in this study. 相似文献
197.
198.
Andrews DM Chow MT Ma Y Cotterell CL Watt SV Anthony DA Akira S Iwakura Y Trapani JA Zitvogel L Smyth MJ 《Immunology and cell biology》2011,89(6):739-746
Toll-like receptor-4-lipopolysaccharide (LPS)-mediated inflammation is used to delineate signals involved in cross-talk between antigen-presenting cells (APCs) and lymphocytes such as natural killer (NK) cells. Following APC stimulation and cytokine release, NK cells produce interferon (IFN)-γ. High levels of LPS induce endotoxicosis, a systemic inflammatory disease in which IFN-γ causes significant morbidity and mortality. Several studies have highlighted the role of interleukin (IL)-18, IL-1β, IL-17A and IFN-γ in the development of endotoxicosis, but whether these cytokines interact with each other is yet to be determined. Our data demonstrate that IL-18 and IL-17A have important roles in NK cell IFN-γ production during endotoxicosis. Importantly, we provide the first evidence that IL-18 also has a role in IL-17A production by T-cell receptor (TCR)-δ cells. Furthermore, we demonstrate that IL-18-deficient mice have a defect in γδ T-cell homeostasis and IL-1β production, both of which can contribute to the development of disease through induction of IL-17A. These results reveal novel requirements for IL-18 in innate immune cell homeostasis and activation, demonstrating that the role of IL-18 in innate immunity occurs at a level other than activation. 相似文献
199.
Mittapalli GK Jackson A Zhao F Lee H Chow S McKelvy J Wong-Staal F Macdonald JE 《Bioorganic & medicinal chemistry letters》2011,21(22):6852-6855
Novel, highly potent small molecule HCV entry inhibitors are reported. The SAR exploration of a hit molecule identified from screening of a compound library led to the identification of highly potent compounds with IC(50) values of <5 nM in the tissue culture HCV infectious assay. 相似文献
200.
Chow R Lin A Tonai R Bolanos R Connor C Mendoza A Heminger R Chow M Ho E Kang J Gindy L Fu C Rao A Gau JF Wang BC Klich I Ratajczak J Ratajczak M Petz LD 《Cytotherapy》2011,13(9):1105-1119
Background aimsLimited cell dose has hampered the use of cord blood transplantation (CBT) in adults. One method of minimizing nucleated cell loss in cord blood (CB) processing is to deplete or reduce plasma but not red blood cells - plasma depletion/reduction (PDR).MethodsThe nucleated cell loss of PDR was studied, and determined to be less than 0.1% in the discarded supernatant plasma fraction in validation experiments. After testing and archival sampling, the median nucleated cell recovery for PDR processing was 90%, and median CD34+ cell recovery 88%. In a CB bank inventory of 12 339 products with both pre- and post-processing total nucleated cells (TNC), PDR processing resulted in median post-processing TNC recoveries of 90.0% after testing and archival samples removal. Using the same 10 CB units divided into two halves, we compared directly the recovery of PDR against hydroxyethyl starch red cell reduction (RCR) for TNC, CD34+ cells and colony-forming units (CFU-GM, CFU-E, CFU-GEMM and total CFU) after parallel processing. We also compared the loss of very small embryonic-like stem cells (VSEL).ResultsWe demonstrated significantly higher recoveries using PDR for TNC (124%), CD34+ cells (121%), CFU-GM (225%), CFU-GEMM (201%), total CFU (186%) and VSEL (187%). The proportion of high TNC products was compared between 10 912 PDR and 38 819 RCR CB products and found to be 200% higher for products that had TNC ≥150 × 107 (P = 0.0001) for the PDR inventory.ConclusionsOur data indicate that PDR processing of CB provides a significantly more efficient usage of this valuable and scarce resource. 相似文献