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Stéphanie Godet Josiane Hérault Gaëlle Pencreac’h Françoise Ergan Céline Loiseau 《Journal of applied phycology》2012,24(6):1547-1553
Microalgae constitute a novel study area for characterising new lipolytic enzymes of biotechnological interest. A new gene from the microalga Isochrysis galbana has been isolated and a preliminary characterisation performed. The full-length cDNA contains 2,060 base pairs with an open reading frame of 1,374 nucleotides encoding a polypeptide of 457 amino acids with a predicted molecular mass of 49 kDa, and a theoretical pI of 5.65. The deduced protein includes highly conserved motifs found in α/β fold hydrolases, and shares some similarities with putative or known lipases. Sequence comparison indicated that the catalytic triad corresponds to residues Ser188, Asp306 and His391, with the nucleophilic residue Ser188 positioned within the consensus G-X-S188-X-G pentapeptide. Phylogenetic analyses established close relationships with fungal lipases and microalgal sequences. 相似文献
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JeanBaptiste Juhel Virginie Marques Andrea Polanco Fernndez Giomar H. BorreroPrez Maria Mutis Martinezguerra Alice Valentini Tony Dejean Stphanie Manel Nicolas Loiseau Laure Velez Rgis Hocd Tom B. Letessier Eilísh Richards Florine Hadjadj Sandra Bessudo Felipe Ladino Camille Albouy David Mouillot Loïc Pellissier 《Ecology and evolution》2021,11(7):2956
- Monitoring large marine mammals is challenging due to their low abundances in general, an ability to move over large distances and wide geographical range sizes.
- The distribution of the pygmy (Kogia breviceps) and dwarf (Kogia sima) sperm whales is informed by relatively rare sightings, which does not permit accurate estimates of their distribution ranges. Hence, their conservation status has long remained Data Deficient (DD) in the Red list of the International Union for Conservation of Nature (IUCN), which prevent appropriate conservation measures.
- Environmental DNA (eDNA) metabarcoding uses DNA traces left by organisms in their environments to detect the presence of targeted taxon, and is here proved to be useful to increase our knowledge on the distribution of rare but emblematic megafauna.
- Retrieving eDNA from filtered surface water provides the first detection of the Dwarf sperm whale (Kogia sima) around the remote Malpelo island (Colombia).
- Environmental DNA collected during oceanic missions can generate better knowledge on rare but emblematic animals even in regions that are generally well sampled for other taxa.