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141.
Cauchetier E Loiseau PM Lehman J Rivollet D Fleury J Astier A Deniau M Paul M 《International journal for parasitology》2002,32(8):1043-1051
Atovaquone, an antiparasitic agent, could possibly represent an alternative therapy after relapse following classical treatment for visceral leishmaniasis. Atovaquone-resistant strains were selected in vitro by stepwise drug pressure to study the mechanism of resistance in Leishmania. Characteristics of a promastigote strain resistant to 250 microg/ml of atovaquone were compared with those of the wild type (WT) strain. Resistant strains were shown to have a high level of resistance (45 times). They were stable in drug-free medium for 6 months, and showed no cross-resistance with other antileishmanial drugs. Rhodamine uptake and efflux were studied. They were not modified in the resistant strain, indicating the absence of P-glycoprotein overexpession. The effect of atovaquone on membrane lipidic composition was determined in both WT and atovaquone-resistant promastigotes. Analysis of lipid composition of the atovaquone-resistant strain showed that sterol biosynthesis was decreased in atovaquone-resistant parasites. Cholesterol was found to be the major membrane sterol as opposed to the WT strain. Cholesterol, due to its ordering effect, could decrease membrane fluidity and subsequently block the passage of atovaquone through the membrane. Increased membrane cholesterol content and altered drug membrane fluidity resulted from possible decrease of ergosterol biosynthesis by atovaquone, incorporation of cholesterol by promastigotes in the culture medium, solubilisation of atovaquone by cholesterol and co-passage of the two compounds or influence of dimethylsulfoxide. These results indicate that different cellular alterations may participate in the resistant phenotype, by altering drug membrane permeability. 相似文献
142.
Phenotype frequencies of autosomal minor histocompatibility antigens display significant differences among populations 总被引:1,自引:0,他引:1 下载免费PDF全文
Spierings E Hendriks M Absi L Canossi A Chhaya S Crowley J Dolstra H Eliaou JF Ellis T Enczmann J Fasano ME Gervais T Gorodezky C Kircher B Laurin D Leffell MS Loiseau P Malkki M Markiewicz M Martinetti M Maruya E Mehra N Oguz F Oudshoorn M Pereira N Rani R Sergeant R Thomson J Tran TH Turpeinen H Yang KL Zunec R Carrington M de Knijff P Goulmy E 《PLoS genetics》2007,3(6):e103
Minor histocompatibility (H) antigens are allogeneic target molecules having significant roles in alloimmune responses after human leukocyte antigen–matched solid organ and stem cell transplantation (SCT). Minor H antigens are instrumental in the processes of transplant rejection, graft-versus-host disease, and in the curative graft-versus-tumor effect of SCT. The latter characteristic enabled the current application of selected minor H antigens in clinical immunotherapeutic SCT protocols. No information exists on the global phenotypic distribution of the currently identified minor H antigens. Therefore, an estimation of their overall impact in human leukocyte antigen–matched solid organ and SCT in the major ethnic populations is still lacking. For the first time, a worldwide phenotype frequency analysis of ten autosomal minor H antigens was executed by 31 laboratories and comprised 2,685 randomly selected individuals from six major ethnic populations. Significant differences in minor H antigen frequencies were observed between the ethnic populations, some of which appeared to be geographically correlated. 相似文献
143.
The unexpected increase of clotrimazole apparent solubility using randomly methylated β‐cyclodextrin 下载免费PDF全文
Bénédicte Pradines Jean‐François Gallard Bogdan I. Iorga Claire Gueutin Gilles Ponchel Philippe M. Loiseau Kawthar Bouchemal 《Journal of molecular recognition : JMR》2015,28(2):96-102
Clotrimazole (CTZ) and cyclodextrin (CD) inclusion complexes having improved apparent water solubility were obtained from phase solubility diagrams. β‐CD (1.5% w/w) and hydroxypropyl‐β‐CD (40% w/w) offered poor CTZ solubility enhancements (12 and 384 times, respectively). Unexpectedly, the apparent solubility of CTZ was 9980 times increased from 0.4 µg.mL?1 (1.42 μM) without CD to 4.89 mg.mL?1 (14.9 mM) using randomly‐methylated β‐CD (Me‐β‐CD) (40% w/w). This is the highest apparent CTZ solubility improvement ever reported in the literature using conventional CDs. Quantitative nuclear magnetic resonance (1H‐NMR) coupled with two‐dimensional nuclear Overhauser effect (NOESY) experiments and molecular docking calculations showed that the highest interactions with Me‐β‐CD were reported for CTZ two phenyl groups. A lower interaction was reported for chlorophenyl, while imidazole had the weakest interaction with Me‐β‐CD. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
144.
The paper explores the capability of [(dppf)Pt(H-nbu2-DTO)]Cl (2) (dppf = 1,1′-diphenylphosphinoferrocene; H-nbu2-DTO = di-nbutyl-dithioxamidate) to act as a starting module for heterometallic linear chains. Actually, the reaction of 2 with [RuCl2(p-cymene)]2 affords the heterotrimetallic complex [Cl(p-cymene) Ru(μ-nbu2-DTO κ-N,N Ru κ-S,S Pt)Pt(dppf κ-P,P Pt) ]2 (4). However 2, allowed to stand, provides a blue compound of formula [(dppf)Pt(H-nbu2-DTO)]nCln (3), the most reliable value of n being 6. The oxidation behavior of the new species 2-4 has also been investigated. In particular, the oxidation behavior of cyclic compound 3 is quite unusual, and suggests a large delocalization of the HOMO over the whole multicomponent molecule. 相似文献
145.
146.
Ana PM Oliveira Rosana Gentile Arnaldo Maldonado Júnior Eduardo J Lopes Torres Silvana C Thiengo 《Memórias do Instituto Oswaldo Cruz》2015,110(6):739-744
The aim of this study was to analyse the infection dynamics ofAngiostrongylus
cantonensis in its possible intermediate hosts over two years in an urban
area in the state of Rio de Janeiro where the presence ofA.
cantonensis had been previously recorded in molluscs. Four of the seven
mollusc species found in the study were exotic.Bradybaena similaris
was the most abundant, followed byAchatina fulica,
Streptaxis sp., Subulina octona,
Bulimulus tenuissimus, Sarasinula linguaeformis
and Leptinaria unilamellata. Only A. fulica
and B. similaris were parasitised by A.
cantonensis and both presented co-infection with other helminths. The
prevalence of A. cantonensisin A. fulica was more
than 50% throughout the study. There was an inverse correlation between the
population size ofA. fulica and the prevalence of A.
cantonensis and abundance of the latter was negatively related to
rainfall. The overall prevalence of A. cantonensis in B.
similariswas 24.6%. A. fulica was the most important
intermediary host of A. cantonensis in the studied area
andB. similaris was secondary in importance for A.
cantonensis transmission dynamics. 相似文献
147.
Etienne Loiseau Gladys Massiera Simon Mendez Patricia?Aguilar Martinez Manouk Abkarian 《Biophysical journal》2015,108(11):2623-2632
Sickle cell anemia is a blood disorder, known to affect the microcirculation and is characterized by painful vaso-occlusive crises in deep tissues. During the last three decades, many scenarios based on the enhanced adhesive properties of the membrane of sickle red blood cells have been proposed, all related to a final decrease in vessels lumen by cells accumulation on the vascular walls. Up to now, none of these scenarios considered the possible role played by the geometry of the flow on deposition. The question of the exact locations of occlusive events at the microcirculatory scale remains open. Here, using microfluidic devices where both geometry and oxygen levels can be controlled, we show that the flow of a suspension of sickle red blood cells around an acute corner of a triangular pillar or of a bifurcation, leads to the enhanced deposition and aggregation of cells. Thanks to our devices, we follow the growth of these aggregates in time and show that their length does not depend on oxygenation levels; instead, we find that their morphology changes dramatically to filamentous structures when using autologous plasma as a suspending fluid. We finally discuss the possible role played by such aggregates in vaso-occlusive events. 相似文献
148.
Clackers M Coe DM Demaine DA Hardy GW Humphreys D Inglis GG Johnston MJ Jones HT House D Loiseau R Minick DJ Skone PA Uings I McLay IM Macdonald SJ 《Bioorganic & medicinal chemistry letters》2007,17(17):4737-4745
Starting from an established series of non-steroidal glucocorticoid receptor (GR) agonists, a large array was designed where a metabolically labile benzoxazinone moiety was replaced. Initial hits bound to GR but lacked agonist activity. Following two further iterations, potent GR agonists were discovered with 20D1E1 having NFkappaB agonism pIC(50) 8.8 (103%). Other analogues such as 23D1E1 display a dissociated profile (NFkappaB pIC(50) 8.1 (103%), MMTV pEC(50) 7.02 (36%)). The tetrahydronaphthalene moiety can also be replaced with substituted aryls such as 24E1 and 25E1. 相似文献
149.
Khaled Khoufache Olivier Puel Nicolas Loiseau Marcel Delaforge Danièle Rivollet André Coste Catherine Cordonnier Estelle Escudier Françoise Botterel Stéphane Bretagne 《BMC microbiology》2007,7(1):5
Background
The role of Aspergillus fumigatus mycotoxins in the colonization of the respiratory tract by conidia has not been studied extensively, even though patients at risk from invasive aspergillosis frequently exhibit respiratory epithelium damage. In a previous study, we found that filtrates of A. fumigatus cultures can specifically alter the electrophysiological properties of human nasal epithelial cells (HNEC) compared to those of non pathogenic moulds. 相似文献150.