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21.
Extensification modify the C and N cycles in grassland ecosystems, but it is not clear whether reduced exploitation increases or decreases soil nitrogen availability, and whether these changes result from a direct effect of the treatment or from an indirect treatment effect through a change in plant species composition. A formerly intensively exploited grassland was submitted to the following treatments: (i) control with one mowing and four grazing periods per year (4G+M), (ii) cessation of mowing (4G), (iii) cessation of mowing and suppression of three grazing periods (1G). During the 13th year of the experiment, the species composition and key indicators of the C and N cycles were measured, using 20 samples per treatment. Lolium perenne and Trifolium repens disappeared in favor of tall caespitose grasses in 4G, and of rhizomatous species in 1G. The species composition and the nitrate concentration of the soil solution suggested an increase in nutrient availability under reduced exploitation, whereas the nitrification and denitrification potentials decreased. More particulate organic matter accumulated in proportion to the below-ground phytomass, whereas the C:N ratio remained constant. Testing treatment effect at similar species composition and plant community effect within the same treatment showed that: (1) the increase in POM residence time was mainly due to the changes in species composition, (2) the decrease in nitrification activity resulted mainly from a direct effect of the treatment, and (3) a compensation between a direct positive and an indirect negative effect of the treatment resulted in no change in extractable N. All results suggested that soil N availability was not decreased, although litter degradability decreased.
Zusammenfassung
Ein Unterlassen der Heuernte und eine Reduktion der Beweidungsintensität ändern die C- und N-Kreisläufe in Grünland-Ökosystemen. Bislang ist unklar, ob eine extensive Nutzung die Nährstoffverfügbarkeit erhöht oder senkt, und ob diese Änderung der Nährstoffverfügbarkeit in direkter Verbindung mit der verringerten Nutzung steht, oder indirekt ein Resultat der sich ändernden botanischen Zusammensetzung ist. In der vorliegenden Untersuchung wurden die botanische Zusammensetzung sowie die C- und N-Kreisläufe im Boden eines naturnahen, ehemals intensiv beweideten Grünlands nach 12 Jahren unterschiedlicher Nutzungsintensitäten – eine Heuernte und vier Beweidungen jährlich (4G+M), viermalige (4G) und einmalige (1G) jährliche Beweidung – verglichen. Pro Weide wurden je 20 Punkte beprobt. Bei verringerter Nutzung waren die Nitrifikation/Denitrifikation geringer als bei intensiver Nutzung. Die botanische Zusammensetzung sowie die Nitrat-Konzentration im Bodenwasser wurden mehr eutroph. So wurden Lolium perenne und Trifolium repens durch horstbildende Grässer (4G) bzw. rhizombildende Arten (1G) abgelöst. Bei extensiver Nutzung war das Verhältnis von organischer Bodenmasse (particle organic matter, POM) zu unterirdischen pflanzlicher Biomasse höher, hatte jedoch keinen Einfluss auf das C/N-Verhältnis. Ein Vergleich von Weiden gleicher botanischer Zusammensetzung (Nutzungs-Effekt) und von Weiden gleicher Nutzung aber unterschiedlicher botanischer Zusetzung (plant-community-Effekt) zeigte: (i) eine Zunahme der POM-Verweildauer in Folge einer veränderten botanischen Zusammensetzung, (ii) eine Abnahme der Nitrifikation in Folge der verringerten Nutzung und (iii) Kompensation der verringerten Nutzung durch eine Änderung der botanischen Zusammensetzung, womit der leicht verfügbare N unverändert blieb. Eine abnehmende Streu-Qualität scheint keinen Einfluss auf das N-Nachlieferungsvermögen zu haben. Eine hohe N-Verfügbarkeit in Verbindung mit geringer Streu Zersetzung scheinen verantwortlich für eine funktionelle Änderung des Ökosystems und seiner Biodiversität. 相似文献22.
A linkage map for the honeybee (Apis mellifera) was constructed mainly from the progeny of two hybrid queens (A. m. ligustica x A. m. mellifera). A total of 541 loci were mapped; 474 were microsatellite loci; a few were additional bands produced during PCRs, one of the two rDNA loci (using ITS), the MDH locus, and three sex-linked markers (Q and FB loci and one RAPD band). Twenty-four linkage groups were estimated of which 5 were minute (between 7.1 and 22.8 cM) and 19 were major groups (>76.5 cM). The number of major linkage groups exceeded by three the number of chromosomes of the complement (n = 16). The sum of the lengths of all linkage groups amounts to 4061 cM to which must be added at least 320 cM to link groups in excess, making a total of at least 4381 cM. The length of the largest linkage group I was 630 cM. The average density of markers was 7.5 cM and the average resolution was about one marker every 300 kb. For most of the large groups, the centromeric region was determined genetically, as described in (accompanying article in this issue), using half-tetrad analysis of thelytokous parthenogens in which diploid restoration occurs through central fusion. Several cases of segregation distortion that appreared to result from deleterious recessives were discovered. A low positive interference was also detected. 相似文献
23.
Foucaud J Jourdan H Le Breton J Loiseau A Konghouleux D Estoup A 《Evolution; international journal of organic evolution》2006,60(8):1646-1657
A unique reproductive system has previously been described in Wasmannia auropunctata, a widespread invasive ant species, where males are produced clonally, female queens are parthenogens, and female workers are produced sexually. However, these findings were mostly based on samples originating from only a limited part of the native range of the species in South America. We used microsatellite markers to uncover the reproductive modes displayed by a large number of nests collected in various invasive W. auropunctata populations introduced 40 years ago into New Caledonia, where the species now forms a single 450-km-long supercolony. Although the main reproduction system in New Caledonia remained clonality for both male and female reproductives, we found evidence of rare sexual reproduction events that led to the production of both new queen and male clonal lineages. All clonal lineages observed in New Caledonia potentially derived from sexual reproduction, recombination, and mutation events from a single female and a single male genotype. Hence, the male and female gene pools are not strictly separated in New Caledonia and the two sexes do not follow independent evolutionary trajectories. Our results also suggest genetic determination for both parthenogenesis and caste. We discuss the evolutionary implications of the emergence of sex in the clonal reproduction system of introduced populations of W. auropunctata. 相似文献
24.
A single autonomous video camera was used to record the abundances of Chromis viridis over a branching Acropora sp. colony eight times per day over a period of 50 days. The poor explanatory power of global radiation suggests the need for recording the light really available to the fish, especially in the UV range. The increasing number of C. viridis observed with increasing wind along shore and water level may correspond to individuals swimming further from their shelter in order to get closer to the food carried by the water currents. 相似文献
25.
Gohil VM Brahmbhatt KG Loiseau PM Bhutani KK 《Bioorganic & medicinal chemistry letters》2012,22(12):3905-3907
β-carbolines from various natural and synthetic sources have been known to show diverse biological activities. As a part of our current ongoing project to search for potent natural product-derived anti-leishmanial compounds, we have synthesized a series of substituted 1-aryl-β-carboline derivatives. A total of 22 compounds were synthesized and tested in vitro against Leishmania donovani, out of which 6 compounds (4, 5, 10, 11, 19 and 22) showed notably more activity than the standard miltefosine (IC(50) 12.07±0.82 μM), with compound 4 being the most potent (IC(50) 2.16±0.26 μM). 相似文献
26.
Olivier Rey Arnaud Estoup Merav Vonshak Anne Loiseau Simon Blanchet Luis Calcaterra Lucila Chifflet Jean‐Pierre Rossi Gaël J. Kergoat Julien Foucaud Jérôme Orivel Maurice Leponce Ted Schultz Benoit Facon 《Ecology letters》2012,15(11):1266-1275
Evolution may improve the invasiveness of populations, but it often remains unclear whether key adaptation events occur after introduction into the recipient habitat (i.e. post‐introduction adaptation scenario), or before introduction within the native range (i.e. prior‐adaptation scenario) or at a primary site of invasion (i.e. bridgehead scenario). We used a multidisciplinary approach to determine which of these three scenarios underlies the invasion of the tropical ant Wasmannia auropunctata in a Mediterranean region (i.e. Israel). Species distribution models (SDM), phylogeographical analyses at a broad geographical scale and laboratory experiments on appropriate native and invasive populations indicated that Israeli populations followed an invasion scenario in which adaptation to cold occurred at the southern limit of the native range before dispersal to Israel. We discuss the usefulness of combining SDM, genetic and experimental approaches for unambiguous determination of eco‐evolutionary invasion scenarios. 相似文献
27.
Marcade M Bourdin J Loiseau N Peillon H Rayer A Drouin D Schweighoffer F Désiré L 《Journal of neurochemistry》2008,106(1):392-404
Pharmacological modulation of the GABAA receptor has gained increasing attention as a potential treatment for central processes affected in Alzheimer disease (AD), including neuronal survival and cognition. The proteolytic cleavage of the amyloid precursor protein (APP) through the α-secretase pathway decreases in AD, concurrent with cognitive impairment. This APP cleavage occurs within the β-amyloid peptide (Aβ) sequence, precluding formation of amyloidogenic peptides and leading to the release of the soluble N-terminal APP fragment (sAPPα) which is neurotrophic and procognitive. In this study, we show that at nanomolar-low micromolar concentrations, etazolate, a selective GABAA receptor modulator, stimulates sAPPα production in rat cortical neurons and in guinea pig brains. Etazolate (20 nM–2 μM) dose-dependently protected rat cortical neurons against Aβ-induced toxicity. The neuroprotective effects of etazolate were fully blocked by GABAA receptor antagonists indicating that this neuroprotection was due to GABAA receptor signalling. Baclofen, a GABAB receptor agonist failed to inhibit the Aβ-induced neuronal death. Furthermore, both pharmacological α-secretase pathway inhibition and sAPPα immunoneutralization approaches prevented etazolate neuroprotection against Aβ, indicating that etazolate exerts its neuroprotective effect via sAPPα induction. Our findings therefore indicate a relationship between GABAA receptor signalling, the α-secretase pathway and neuroprotection, documenting a new therapeutic approach for AD treatment. 相似文献
28.
H Kayaba D Dombrowicz G Woerly J P Papin S Loiseau M Capron 《Journal of immunology (Baltimore, Md. : 1950)》2001,167(2):995-1003
FcepsilonRI expressed by human eosinophils is involved in IgE-mediated cytotoxicity reactions toward the parasite Schistosoma mansoni in vitro. However, because receptor expression is low on these cells, its functional role is still controversial. In this study, we have measured surface and intracellular expression of FcepsilonRI by blood eosinophils from hypereosinophilic patients and normal donors. The number of unoccupied receptors corresponded to approximately 4,500 Ab binding sites per cell, whereas 50,000 Ab binding sites per cell were detected intracellularly. Eosinophils from patients displayed significantly more unoccupied receptors than cells from normal donors. This number correlated to both serum IgE concentrations and to membrane-bound IgE. The lack of FcepsilonRI expression by mouse eosinophils has hampered further studies. To overcome this fact and experimentally confirm our findings on human eosinophils, we engineered IL-5 x hFcepsilonRIalpha double-transgenic mice, whose bone marrow, blood, spleen, and peritoneal eosinophils expressed FcepsilonRI levels similar to levels of human eosinophils, after 4 days culture with IgE in the presence of IL-5. Both human and mouse eosinophils were able to secrete IL-10 upon FcepsilonRI engagement. Thus, comparative analysis of cells from patients and from a relevant animal model allowed us to clearly demonstrate that FcepsilonRI-mediated eosinophil activation leads to IL-10 secretion. Through FcepsilonRI expression, these cells are able to contribute to both the regulation of the immune response and to its effector mechanisms. 相似文献
29.
Characterization of nine polymorphic microsatellite loci in the fungus Botrytis cinerea (Ascomycota)
E. Fournier T. Giraud A. Loiseau D. Vautrin A. Estoup M. Solignac J. M. Cornuet Y. Brygoo 《Molecular ecology resources》2002,2(3):253-255
Nine microsatellite markers were characterized in the fungus Botrytis cinerea. Genomic DNA sequences from the partial sequencing of 12 000 bacterial artificial chromosome (BAC) clones, were screened by BLAST for various microsatellite motives, and primer pairs were designed. Cross‐amplification and polymorphism were assessed on 49 isolates from B. cinerea and two related species, collected from natural populations on several plants and locations. 相似文献
30.
Nicolas Loiseau Florine Cavelier Jean-Pierre Noel Jean-Marie Gomis 《Journal of peptide science》2002,8(7):335-346
Tentoxin is a naturally occurring phytotoxic cyclic tetrapeptide excreted by fungi of the Alternaria alternata family. The four total syntheses of tentoxin published to date give poor total yields, mainly owing to two difficulties, the introduction of the dehydro amino acid and more especially the cyclization step. Here we describe a method that stereospecifically introduces Z-dehydrophenylalanine (deltaZPhe) by a modified Erlenmeyer aldolization reaction. The linear tetrapeptide, Boc-R1Ala-Leu-R2deltaZPhe-G1y-OMe (R1, R2: CH3, 14CH3), the precursor of tentoxin, was obtained in a 72% yield from Boc-Leu-Gly-OH. This linear tetrapeptide, labelled with carbon-14, was used for a comparative study of four cyclization reagents DPPA, DCC-PfpOH, HBTU and HATU. This last was the most effective and gave tentoxin in a 81% cyclization yield. The activated ester formed with this reagent displayed an enhanced capacity for cyclization, permitting cyclization in concentrated medium (10 mM). This new synthetic route gave tentoxin in a 60% yield from Boc-Leu-Gly-OH and offers a means of achieving the synthesis of hitherto elusive analogues. 相似文献