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排序方式: 共有144条查询结果,搜索用时 250 毫秒
51.
Aggressive amyloidosis in mice expressing human amyloid peptides with the Arctic mutation 总被引:4,自引:0,他引:4
The Arctic mutation within the amyloid-beta (Abeta) peptide causes Alzheimer disease. In vitro, Arctic-mutant Abeta forms (proto)fibrils more effectively than wild-type Abeta. We generated transgenic mouse lines expressing Arctic-mutant human amyloid precursor proteins (hAPP). Amyloid plaques formed faster and were more extensive in Arctic mice than in hAPP mice expressing wild-type Abeta, even though Arctic mice had lower Abeta(1-42/1-40) ratios. Thus, the Arctic mutation is highly amyloidogenic in vivo. 相似文献
52.
氧自由基致豚鼠心室肌细胞跨膜电位变化的离子电流基础 总被引:7,自引:0,他引:7
徐长庆 《中国应用生理学杂志》2000,16(2):121-124
目的:旨在提示氧自由基参与缺血/再灌注性心委失常发生的离子电流基础。方法:采用膜片钳全细胞式记录技术,观察H2O2(1mmol/L)对豚鼠心室肌细胞跨膜电位和相关离子电流的影响。结果:H2O2使豚鼠心肌单细胞的静息电位(RP)降低,动作电位时程(ASD)显著缩短,对动作电位幅度(APA)和超射(OS)及钠电流的峰值(INa)均无明显影响;明显抑制内向整流钾电流(IK1),尤其在超极化时;增强延迟外 相似文献
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D. Ll. Griffiths 《BMJ (Clinical research ed.)》1952,1(4759):645-647
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Joan Ll. Vives-Corrons Josep M. Jou Anna Ester Maria Ibars Josep Carreras Ramon Bartrôns Fernando Climent Santiago Crisolía 《Biochemical and biophysical research communications》1981,103(1):111-117
Incubation of blood with vanadate markedly increases the affinity of hemoglobin for oxygen, decreases the deformability of erythrocytes, reduces their osmotic fragility and alters their morphology, determining the appearance of equinocytic forms. Since vanadate is easily taken up by the erythrocytes and binds hemoglobin, these effects might result from interactions of vanadate with hemoglobin and with membrane proteins at the glycerate-2, 3-P2 and/or ATP binding site. In addition, vanadate inhibits phosphoglycerate mutase, phosphoglucomutase and adenylate-kinase activities from hemolysates, suggesting a possible inhibitory effect on erythrocyte metabolism 相似文献
56.
Vardakou M Nueno Palop C Gasson M Narbad A Christakopoulos P 《International journal of biological macromolecules》2007,41(5):584-589
In vitro fermentations were carried out by using a model of the human colon to stimulate microbial activities of gut bacteria. The model consisted of a three-stage culture system. Bacterial populations were evaluated under the effect of three types of arabinoxylan, a nonstarch polysaccharide derived from wheat, the water-unextractable arabinoxylan fraction (WU-AX), WU-AX pretreated with exogenous xylanase and the soluble water-extractable arabinoxylan fraction (WE-AX). The xylanase pretreated (WU-AX) had a stimulatory effect upon colonic bifidobacteria throughout all three vessels. Counts of Bacteroides spp. and Clostridium spp. were also both significantly reduced. Addition of the WU-AX substrates to the first vessel resulted in induction of bacterial synthesis of extracellular hydrolytic enzymes xylanase and ferulic acid esterase which are both required for bacterial metabolism of WU-AX; this induction was significantly greater with the xylanase treated WU-AX. 相似文献
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María Asunción García-González David Nicolás-Pérez Angel Lanas Luis Bujanda Patricia Carrera Rafael Benito Mark Strunk Federico Sope?a Santos Santolaria Elena Piazuelo Pilar Jiménez Rafael Campo Jesús Espinel Marisa Manzano Fernando Geijo María Pellisé Ferrán González-Huix Jorge Espinós Manuel Zaballa Llúcia Titó Luis Barranco Roberto Pazo Enrique Quintero 《PloS one》2012,7(9)
Background
Genetic factors influencing the prognosis of gastric adenocarcinoma (GAC) are not well known. Given the relevance of cytokines and other pro-inflammatory mediators in cancer progression and invasiveness, we aimed to assess the prognostic role of several functional cytokine and cyclooxygenase gene polymorphisms in patients with GAC.Methodology
Genomic DNA from 380 Spanish Caucasian patients with primary GAC was genotyped for 23 polymorphisms in pro-inflammatory (IL1B, TNFA, LTA, IL6, IL12p40), anti-inflammatory (IL4, IL1RN, IL10, TGFB1) cytokine, and cyclooxygenase (PTGS1 and PTGS2) genes by PCR, RFLP and TaqMan assays. Clinical and histological information was collected prospectively. Survival curves were estimated by the Kaplan-Meier method and compared using the log rank test. Outcome was determined by analysis of Cox proportional hazards, adjusting for confounding factors.Results
The median follow-up period and median overall survival (OS) time were 9.9 months (range 0.4–120.3) and 10.9 months (95% CI: 8.9–14.1), respectively. Multivariate analysis identified tumor stages III (HR, 3.23; 95% CI:2–5.22) and IV (HR, 5.5; 95% CI: 3.51–8.63) as independent factors associated with a significantly reduced OS, whereas surgical treatment (HR: 0.44; 95%CI: 0.3–0.6) was related to a better prognosis of the disease. Concerning genetic factors, none of the 23 polymorphisms evaluated in the current study did influence survival. Moreover, no gene-environment interactions on GAC prognosis were observed.Conclusions
Our results show that, in our population, the panel of selected pro- and anti-inflammatory cytokine, and cyclooxygenase gene polymorphisms are not relevant in determining the prognosis of gastric adenocarcinoma. 相似文献58.
Genetic diversity of different apricot geographical groups determined by SSR markers. 总被引:9,自引:0,他引:9
Carlos Romero Andrzej Pedryc Verónica Mu?oz Gerardo Llácer Maria Luisa Badenes 《Génome》2003,46(2):244-252
Forty apricot cultivars with different geographic origins belonging to the germplasm collections of St. Istvan University (Budapest, Hungary) and the Instituto Valenciano de Investigaciones Agrarias (IVIA) (Valencia, Spain) were studied by means of SSR markers. The aim of the study was to determine the genetic relationships among genotypes from different eco-geographical groups. Sixteen primer pairs flanking microsatellite sequences in the peach genome were assayed. Eleven of them were polymorphic in the set of cultivars studied and allowed every genotype to be unambiguously distinguished. Genetic diversity in the population studied was analyzed using several variability parameters. A total of 34 alleles were detected with a mean value of 3.1 alleles/locus. The expected heterozygosity mean was 0.46 and the observed heterozygosity was 32% on an average leading to a high value of the Wright's fixation index (0.32). Additionally, UPGMA cluster analysis based on Nei's genetic distance grouped genotypes according to their geographic origins and pedigrees. SSR markers have proved to be an efficient tool for fingerprinting cultivars and conducting genetic-diversity studies in apricot. 相似文献
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