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241.
Ljiljana Tubić Goran Anačkov Jelena Milojević Nabil Ghalawenji Nevena Mitić Ružica Igić Snežana Zdravković-Korać 《Plant Cell, Tissue and Organ Culture》2014,118(1):101-110
This study assessed the shoot regeneration capacity of root-tips isolated from single seed-derived individual plants, obtained from a wild, open-pollinated micropopulation of shallot (Allium ascalonicum). Considerable variation was observed in the regeneration capacities of individual lines, ranging from 0.93 to 100 %, and a mean bud number per root explant between 0.09 and 20.67. One line was found to be superior, and was chosen for protocol optimization, focusing on the 2,4-D/BA ratio, duration of the CI phase and light conditions. The application of the optimized protocol to other lines, selected for their variable regeneration capacities, enhanced the process of regeneration and shortened the time required to obtain healthy plantlets, even in inferior lines. However, highly responsive lines remained superior, indicating that differences at the individual level must not be overlooked. The conditions employed in this study may serve as a base to facilitate the application of molecular breeding methods in shallot. 相似文献
242.
It is well known that there is heterogeneity of mitochondrial DNA (mtDNA) within and among natural populations of same species.
The polymorphism level of particular regions of mtDNA gives valuable results in detection of population genetic structure.
The aim of this paper was to detect polymorphism of three mtDNA regions: cytochrome oxidase I (COI), Control region, and 12S/16S
rRNA, by the mtDNA RFLP-PCR method, in three Lepus europaeus populations from Vojvodina province (Serbia and Montenegro). Polymorphism was detected within the two regions, COI and Control
region, while 12S/16S rRNA region was monomorphic in all 77 individuals. Eight haplotypes were detected in the brown hare
population in Vojvodina, and three were unique for the Srem brown hare population. 相似文献
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Predrag Sikiric Sven Seiwerth Gorana Aralica Darko Perovic Mario Staresinic Tomislav Anic Miroslav Gjurasin Ingrid Prkacin Jadranka Separovic Dinko Stancic-Rokotov Martina Lovric-Bencic Darko Mikus Branko Turkovic Ivo Rotkvic Stjepan Mise Rudolf Rucman Marijan Petek Tihomil Ziger Bozidar Sebecic Zoran Ivasovic Vjekoslav Jagic Ljiljana Komericki Ivan Balen Alenka Boban-Blagaic Ivo Sjekavica 《Journal of Physiology》2001,95(1-6):283-288
After demonstration that cysteamine induced duodenal lesions in gastrectomized rats, while a number of antiulcer drugs mitigated these lesions, it was shown that one single intrarectal (i.r.) cysteamine application produced severe colon lesions in acute studies in rats. Thus, the further focus was on the protracted effect of cysteamine challenge (400 mg/kg b.w. i.r.) and therapy influence in chronic experiments in female rats. Regularly, cysteamine colon lesions were markedly mitigated by ranitidine (10), omeprazole (10), atropine (10), methylprednisolone (1), sulphasalazine (50; mg/kg), pentadecapeptide BPC 157 (PL-10, PLD-116; 10 microg or 10 ng/kg). Specifically, after 1 or 3 months following initial challenge (cysteamine 400 mg/kg i.r.) in female rat, the therapy [BPC 157 (PL-10, PLD-116 (10.0 microg or 10.0 ng/kg; i.g., i.p., i.r.), ranitidine, omeprazole, atropine, methylprednisolone, sulphasalazine (i.p.)] reversed the protracted cysteamine colon injury: the 1 week-regimen (once daily application) started after 1 month post-cysteamine, as well as the 2 weeks-regimen (once daily application), which started after 3 months. The effect on recidive lesion was also tested. These cysteamine lesions may reappear after stopping therapy (after stopping therapy for 3 weeks at the end of 2-weeks regimen started in 3 months-cysteamine female rats) in sulphasalazine group, while this reappearance is markedly antagonized in pentadecapeptide BPC 157 (PL-10, PLD-116)-rats (cysteamine-colon lesion still substantially low). 相似文献