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101.
102.
It has been 45 years since Russell and Burch first proposed the concept of the '3Rs', yet it remains unclear how those individuals involved in animal research view and implement these concepts. The authors used a questionnaire survey to determine how well-known experts judged issues related to the 3Rs. 相似文献
103.
Meeting after meeting: 20 years of discoveries by the members of the Exocytosis–Endocytosis Club 下载免费PDF全文
Florence Niedergang Stéphane Gasman Nicolas Vitale Claire Desnos Christophe Lamaze 《Biology of the cell / under the auspices of the European Cell Biology Organization》2017,109(9):339-353
Twenty years ago, a group of French cell biologists merged two scientific clubs with the aim of bringing together researchers in the fields of Endocytosis and Exocytosis. Founded in 1997, the first annual meeting of the Exocytosis Club was held in 1998. The Endocytosis Club held quarterly meetings from its founding in 1999. The first joint annual meeting of the Exocytosis–Endocytosis Club took place in Paris in April, 2001. What started as a modest gathering of enthusiastic scientists working in the field of cell trafficking has gone from strength to strength, rapidly becoming an unmissable yearly meeting, vividly demonstrating the high quality of science performed in our community and beyond. On the occasion of the 20th meeting of our club, we want to provide historic insight into the fields of exocytosis and endocytosis, and by extension, to subcellular trafficking, highlighting how French scientists have contributed to major advances in these fields. Today, the Exocytosis–Endocytosis Club represents a vibrant and friendly community that will hold its 20th meeting at the Presqu'Ile de Giens, near Toulon in the South of France, on May 11–13, 2017. 相似文献
104.
De Stefano L Vitale A Rea I Staiano M Rotiroti L Labella T Rendina I Aurilia V Rossi M D'Auria S 《Extremophiles : life under extreme conditions》2008,12(1):69-73
The D-trehalose/D-maltose-binding protein (TMBP), a monomeric protein of 48 kDa, is one component of the trehalose and maltose
(Mal) uptake system. In the hyperthermophilic archaeon Thermococcus litoralis, this is mediated by a protein-dependent ATP-binding cassette system transporter. The gene coding for a thermostable TMBP
from the archaeon T. litoralis has been cloned, and the recombinant protein has been expressed in E. coli. The recombinant TMBP has been purified to homogeneity and characterized. It exhibits the same functional and structural
properties as the native one. In fact, it is highly thermostable and binds sugars, such as maltose, trehalose and glucose,
with high affinity. In this work, we have immobilized TMBP on a porous silicon wafer. The immobilization of TMBP to the chip
was monitored by reflectivity and Fourier Transformed Infrared spectroscopy. Furthermore, we have tested the optical response
of the protein-Chip complex to glucose binding. The obtained data suggest the use of this protein for the design of advanced
optical non-consuming analyte biosensors for glucose detection.
The authors wish to dedicate this work to Prof. Ignacy Gryczynski, University of North Texas, TX, USA, for his outstanding
contribution to the development of new sensing methodologies. 相似文献
105.
Perrone MG Santandrea E Bleve L Vitale P Colabufo NA Jockers R Milazzo FM Sciarroni AF Scilimati A 《Bioorganic & medicinal chemistry》2008,16(5):2473-2488
Since it is widely distributed into the body, beta(3)-adrenoceptor is becoming an attractive target for the treatment of several pathologies such as obesity, type 2 diabetes, metabolic syndrome, cachexia, overactive bladder, ulcero-inflammatory disorder of the gut, preterm labour, anxiety and depressive disorders, and heart failure. New compounds belonging to the class of arylethanolamines bearing one or two stereogenic centres were prepared in good yields as racemates and optically active forms. They were, then, evaluated for their intrinsic activity towards beta(3)-adrenoceptor and their affinity for beta(1)- and beta(2)-adrenergic receptors. Stereochemical features were found to play a crucial role in determining the behaviour of such compounds. In particular, alpha-racemic, (alphaR)- and (alphaS)-2-{4-[2-(2-hydroxy-2-phenylethylamino)ethyl]phenoxy}-2- methylpropanoic acid, (alpha-rac, beta-rac)-, (alphaR, betaS)- and (alphaR, betaR)- 2-{4-[2-(2-hydroxy-2-phenylethylamino)ethyl]phenoxy}propanoic acid were found to be endowed with beta(3)-adrenoceptor agonistic activity. Whereas, (alphaS, betaS)- and (alphaS, betaR)-2-{4-[2-(2-hydroxy-2-phenylethylamino)ethyl]phenoxy}propanoic acid behaved as beta(3)-adrenoceptor inverse agonists. Such compounds showed no affinity for beta(1)- and beta(2)-adrenergic receptor, respectively. Thus, resulting highly selective beta(3)-adrenoceptor ligands. 相似文献
106.
Laura Zaccaro Enrico Bucci Rosa Maria Vitale Giuseppe Perretta Roberto Fattorusso Ettore Benedetti Michele Saviano Carlo Pedone 《Journal of peptide science》2003,9(2):90-105
The objective of our study was to mimic in a typical reductionist approach the molecular interactions observed at the interface between the gp130 receptor and interleukin-6 during formation of their complex. A peptide system obtained by reproducing some of the interleukin-6/gp130 molecular interactions into a two-helix bundle structure was investigated. The solution conformational features of this system were determined by CD and NMR techniques. The CD titration experiments demonstrated that the interaction between the designed peptides is specific and based on a well-defined stoichiometry. The NMR data confirmed some of the structural features of the binding mechanism as predicted by the rational design and indicated that under our conditions the recognition specificity and affinity can be explained by the formation of a two-helix bundle. Thus, the data reported herein represent a promising indication on how to develop new peptides able to interfere with formation of the interleukin-6/gp130 complex. 相似文献
107.
Carla Marusic Alessandro Vitale Emanuela Pedrazzini Marcello Donini Lorenzo Frigerio Ralph Bock Philip J. Dix Matthew S. McCabe Michele Bellucci Eugenio Benvenuto 《Transgenic research》2009,18(4):499-512
The first evidence that plants represent a valid, safe and cost-effective alternative to traditional expression systems for
large-scale production of antigens and antibodies was described more than 10 years ago. Since then, considerable improvements
have been made to increase the yield of plant-produced proteins. These include the use of signal sequences to target proteins
to different cellular compartments, plastid transformation to achieve high transgene dosage, codon usage optimization to boost
gene expression, and protein fusions to improve recombinant protein stability and accumulation. Thus, several HIV/SIV antigens
and neutralizing anti-HIV antibodies have recently been successfully expressed in plants by stable nuclear or plastid transformation,
and by transient expression systems based on plant virus vectors or Agrobacterium-mediated infection. The current article gives an overview of plant expressed HIV antigens and antibodies and provides an
account of the use of different strategies aimed at increasing the expression of the accessory multifunctional HIV-1 Nef protein
in transgenic plants. 相似文献
108.
Sergio Papa Vittoria Petruzzella Salvatore Scacco Arcangela Iuso Rita Vitale Domenico De Rasmo Claudia Piccoli Michele Scivetti Teresa Rizza 《BBA》2009,1787(5):502-764
This paper covers genetic and biochemical aspects of mitochondrial bioenergetics dysfunction in hereditary neurological disorders associated with complex I defects. Three types of hereditary complex I dysfunction are dealt with: (i) homozygous mutations in the nuclear genes NDUFS1 and NDUFS4 of complex I, associated with mitochondrial encephalopathy; (ii) a recessive hereditary epileptic neurological disorder associated with enhanced proteolytic degradation of complex I; (iii) homoplasmic mutations in the ND5 and ND6 mitochondrial genes of the complex, cohexistent with mutation in the nuclear PINK1 gene in familial Parkinsonism. The genetic and biochemical data examined highlight different mechanisms by which mitochondrial bioenergetics is altered in these hereditary defects of complex I. This knowledge, besides clarifying molecular aspects of the pathogenesis of hereditary diseases, can also provide hints for understanding the involvement of complex I in sporadic neurological disorders and aging, as well as for developing therapeutical strategies. 相似文献
109.
110.
Andreotti G Vitale RM Avidan-Shpalter C Amodeo P Gazit E Motta A 《The Journal of biological chemistry》2011,286(4):2707-2718
Irreversible aggregation limits bioavailability and therapeutic activity of protein-based drugs. Here we show that an aggregation-resistant mutant can be engineered by structural homology with a non-amyloidogenic analogue and that the aggregation-resistant variant may act as an inhibitor. This strategy has successfully been applied to the amyloidogenic human calcitonin (hCT). Including only five residues from the non-amyloidogenic salmon calcitonin (sCT), we obtained a variant, polar human calcitonin (phCT), whose solution structure was shown by CD, NMR, and calculations to be practically identical to that of sCT. phCT was also observed to be a potent amyloidogenesis inhibitor of hCT when mixed with it in a 1:1 ratio. Fibrillation studies of phCT and the phCT-hCT mixture mimicked the sCT behavior in the kinetics and shapes of the fibrils with a dramatic reduction with respect to hCT. Finally, the effect of phCT alone and of the mixture on the intracellular cAMP level in T47D cells confirmed for the mutant and the mixture their calcitonin-like activity, exhibiting stimulation effects identical to those of sCT, the current therapeutic form. The strategy followed appears to be suitable to develop new forms of hCT with a striking reduction of aggregation and improved activity. Finally, the inhibitory properties of the aggregation-resistant analogue, if confirmed for other amyloidogenic peptides, may favor a new strategy for controlling fibril formation in a variety of human diseases. 相似文献