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71.
Luhmann UF Lange CA Robbie S Munro PM Cowing JA Armer HE Luong V Carvalho LS MacLaren RE Fitzke FW Bainbridge JW Ali RR 《PloS one》2012,7(4):e35551
Microglia and macrophages are recruited to sites of retinal degeneration where local cytokines and chemokines determine protective or neurotoxic microglia responses. Defining the role of Ccl2-Ccr2 and Cx3cl1-Cx3cr1 signalling for retinal pathology is of particular interest because of its potential role in age-related macular degeneration (AMD). Ccl2, Ccr2, and Cx3cr1 signalling defects impair macrophage trafficking, but have, in several conflicting studies, been reported to show different degrees of age-related retinal degeneration. Ccl2/Cx3cr1 double knockout (CCDKO) mice show an early onset retinal degeneration and have been suggested as a model for AMD. In order to understand phenotypic discrepancies in different chemokine knockout lines and to study how defects in Ccl2 and/or Cx3cr1 signalling contribute to the described early onset retinal degeneration, we defined primary and secondary pathological events in CCDKO mice. To control for genetic background variability, we compared the original phenotype with that of single Ccl2, Cx3cr1 and Ccl2/Cx3cr1 double knockout mice obtained from backcrosses of CCDKO with C57Bl/6 mice. We found that the primary pathological event in CCDKO mice develops in the inferior outer nuclear layer independently of light around postnatal day P14. RPE and vascular lesions develop secondarily with increasing penetrance with age and are clinically similar to retinal telangiectasia not to choroidal neovascularisation. Furthermore, we provide evidence that a third autosomal recessive gene causes the degeneration in CCDKO mice and in all affected re-derived lines and subsequently demonstrated co-segregation of the naturally occurring RD8 mutation in the Crb1 gene. By comparing CCDKO mice with re-derived CCl2(-/-)/Crb1(Rd8/RD8), Cx3cr1(-/-)/Crb1(Rd8/RD8) and CCl2(-/-)/Cx3cr1(-/-)/Crb1(Rd8/RD8) mice, we observed a differential modulation of the retinal phenotype by genetic background and both chemokine signalling pathways. These findings indicate that CCDKO mice are not a model of AMD, but a model for an inherited retinal degeneration that is differentially modulated by Ccl2-Ccr2 and Cx3cl1-Cx3cr1 chemokine signalling. 相似文献
72.
Zamira A. Ávila-Valle Alondra Castro-Campillo Livia León-Paniagua Isaías H. Salgado-Ugalde Adolfo G. Navarro-Sigüenza Blanca E. Hernández-Baños José Ramírez-Pulido 《Mammalian Biology》2012,77(3):166-177
Several authors have discussed whether Peromyscus furvus is a monotypic species rather than a polytypic entity, that it includes more than one species. Here, we analyze these questions by means of traditional morphometrics and by genetic analyses using ND3-ND4 mtDNA genes as markers. In spite of a generalized overlap of the measurable characters among populations, our analyses show that the northernmost populations, which was assignable to P. latirostris, consistently show larger dimensions overall. The amount of genetic differentiation revealed by our molecular data, support conclusive evidence to suggest this taxon is a valid species. Our results also disclose that morphometric and molecular segregation between P. furvus and P. angustirostris is still incomplete. Finally, the two populations from the state of Oaxaca showed more morphometric affinity with those attributable to P. furvus and revealed a discrete degree of genetic differentiation. Nevertheless, their systematic position is not clear yet. 相似文献
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74.
Pica-Mattoccia L Orsini T Basso A Festucci A Liberti P Guidi A Marcatto-Maggi AL Nobre-Santana S Troiani AR Cioli D Valle C 《Experimental parasitology》2008,119(3):332-335
The schistosomicidal activity of praziquantel (PZQ) is accompanied by a large influx of calcium into the worms, suggesting that this phenomenon could be the source of the observed muscular contraction, surface disruption and eventual death of the parasite. We have incubated live adult schistosomes in a medium containing radioactive calcium and we were able to confirm that PZQ does indeed stimulate calcium entry into the parasite. An even higher calcium uptake, however, occurred in schistosomes exposed to PZQ after pre-incubation with cytochalasin D, a condition that suppresses PZQ schistosomicidal effects and allows the complete survival of the parasites. The calcium blockers nicardipine and nifedipine also failed to prevent the calcium influx induced by PZQ. Similarly, a large calcium influx occurred in 28-day-old worms exposed to PZQ, in spite of the fact that these immature worms are largely insensitive to the schistosomicidal effects of the drug. Schistosomes incubated overnight with radioactive calcium and PZQ and then returned to normal medium, retained a calcium content higher than worms pre-incubated with cytochalasin D, but the difference could be a consequence—rather than a cause—of schistosomicidal effects. These results suggest that calcium accumulation by itself, at least as measured in whole parasites maintained in vitro, may not represent an exhaustive explanation for the schistosomicidal effects of PZQ. 相似文献
75.
Colletotrichum lindemuthianum, the causal agent of anthracnose in the common bean (Phaseolus vulgaris), presents a wide genetic and pathogenic variability that gives rise to complications in the development of resistant bean
cultivars. The aim of this study was to identify the variability within race 65 of C. lindemuthianum, the race most commonly encountered in Brazil, through randomly amplified polymorphic DNA (RAPD) and anastomosis analyses.
Thirteen isolates of race 65, collected in different years and from various host cultivars located in diverse areas of the
state of Minas Gerais, Brazil, were investigated. Twenty-four RAPD primers were employed and 83 polymorphic bands amplified.
Genetic similarities were estimated from the Sorensen-Dice coefficient and ranged from 0.54 to 0.82. The dendrogram obtained
by cluster analysis classified the isolates into 11 separate groups. For the purposes of the analysis of anastomosis, isolates
were considered to be compatible when the fusion of hyphae from different isolates could be observed. The proportion of compatible
reactions for each isolate was estimated and similarity estimates, based on the Russel & Rao coefficient, ranged from 0.28
to 0.85. Isolates were classified into 11 anastomosis groups, 10 of which were formed by only one isolate. Although isolates
LV61, LV73 and LV58 were classified in the same anastomosis group, they were genetically distinct according to RAPD analysis.
Results from both RAPD and anastomosis analyses revealed great variability within C. lindemuthianum race 65. 相似文献
76.
77.
78.
L. Wanntorp H.-E. Wanntorp B. Oxelman M. Källersjö 《Plant Systematics and Evolution》2001,226(1-2):85-107
The phylogeny of the genus Gunnera is investigated for the first time. Twelve species representing the six currently recognised subgenera are analysed. Two
chloroplast DNA regions, the rbcL gene and the rps16 intron, together provide 46 informative characters out of 2335. A combined analysis of both genes gives four most parsimonious
trees, firmly establishing the east South American G. herteri as sister group to the rest of the genus. The African G. perpensa is sister group to two well-supported clades, one including the South American subgenera Misandra and Panke, the other the Australian/New Zealand/Malayan species of subgenera Milligania and Pseudogunnera. Thus, South America is a composite area for Gunnera, showing up at two different levels in the cladogram. Our analysis supports a close biogeographic relationship between Australia
and New Zealand. The evolution of some morphological characters is discussed. Lastly, the unusual structure of some of the
rbcL sequences is reported.
Received July 6, 2000 Accepted October 24, 2000 相似文献
79.
80.
Livia R. Tanjung Gabrielle Whittle Bruce E. Shaw Garry A. Bloomfield Margaret E. Katz Brian F. Cheetham 《Anaerobe》2009,15(5):219-224
The Gram-negative anaerobic pathogen Dichelobacter nodosus is the principal causative agent of footrot in sheep. The intA, intB and intC elements are mobile genetic elements which integrate into two tRNA genes downstream from csrA (formerly glpA) and pnpA in the D. nodosus chromosome. CsrA homologues act as global repressors of virulence in several bacterial pathogens, as does polynucleotide phosphorylase, the product of pnpA. We have proposed a model in which virulence in D. nodosus is controlled in part by the integration of genetic elements downstream from csrA and pnpA, altering the expression of these putative global regulators of virulence. We describe here a novel integrated genetic element, the intD element, which is 32 kb in size and contains an integrase gene, intD, several genes related to genes on other integrated elements of D. nodosus, a type IV secretion system and a putative mobilisation region, suggesting that the intD element has a role in the transfer of other genetic elements. Most of the D. nodosus strains examined which contained the intD gene were benign, with intD integrated next to pnpA, supporting our previous observation that virulent strains of D. nodosus have the intA element next to pnpA. 相似文献