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181.
182.
Sera from both human cytomegalovirus (HCMV)-seropositive adults and infants with congenital HCMV infection recognized two major HCMV glycoprotein complexes. However, proliferative responses of peripheral blood mononuclear cells to these complexes varied among seropositive adults and were not detected in any of the infants. Thus, these glycoproteins alone may not be sufficient to develop a subviral HCMV vaccine. 相似文献
183.
Chromosome breakage caused by mutagens in male germ cells can be analyzed by micronucleus induction during meiotic division. This can be followed in vitro by culturing seminiferous tubular segments from stages of the epithelial cycle that contain late pachytene and diakinetic primary spermatocytes. We studied the mutagenic potential of a male contraceptive, gossypol, in this test system using adriamycin (10 ng/ml) as a reference mutagen. A small but significant increase in the frequency of micronuclei was induced with concentrations of 10 and 20 micrograms/ml of gossypol, while cytotoxic effects appeared at concentration of 20 micrograms/ml and were evident at 50 micrograms/ml. Analysis of meiotic micronucleus induction in vitro seems to be a sensitive test system of male germ-cell mutagenesis, but further studies on the possible mutagenic effects of gossypol are needed. 相似文献
184.
Sharon A. Boylan Kristin T. Chun Barton A. Edson Chester W. Price 《Molecular & general genetics : MGG》1988,212(2):271-280
Summary The physiological roles of the gene subset defined by early-blocked sporulation mutations (spo0) and their second-site suppressor alleles (rvtA11 and crsA47) remain cryptic for both vegetative and sporulating Bacillus subtilis cells. To test the hypothesis that spo0 gene products affect global regulation, we assayed the levels of carbon- and nitrogen-sensitive enzymes in wild-type and spo0 strains grown in a defined minimal medium containing various carbon and nitrogen sources. All the spo0 mutations (except spo0J) affected both histidase and arabinose isomerase levels in an unexpected way: levels of both carbon-sensitive enzymes were two- to six-fold higher in spo0 strains compared to wild type, when cells were grown on the derepressing carbon sources arabinose or maltose. There was no difference in enzyme levels with glucose-grown cells, nor was there a significant difference in levels of the carbonindependent enzymes glutamine synthetase and glucose-6-phosphate dehydrogenase. This effect was not due to a slower growth rate for the spo0 mutants on the poor carbon and nitrogen sources used. The levels of carbon-sensitive enzymes were not simply correlated with sporulation ability in genetically suppressed spo0 mutants, but the rvtA and crsA suppressors each had such marked effects on wild-type growth and enzyme levels that these results were difficult to interpret. We conclude that directly or indirectly the spo0 mutations, although blocking the sporulation process, increase levels of carbon-sensitive enzymes, possibly at the level of gene expression. 相似文献
185.
Applied anatomy of the anterolateral femoral flap 总被引:13,自引:0,他引:13
D C Xu S Z Zhong J M Kong G Y Wang M Z Liu L S Luo J H Gao 《Plastic and reconstructive surgery》1988,82(2):305-310
A study of the source of the blood supply to the anterolateral femoral flap was carried out on 42 lower limbs of adult cadavers (among them 35 cadavers with injection of red latex and 1 with india ink into the arteries and 6 vascular cast specimens), and the surface locations of the vascular pedicle were detected on 50 healthy adults. It was found that the descending branch of the lateral circumflex femoral vessel was an ideal axial vessel. There are constant perforating branches of the myocutaneous artery or cutaneous branches from the intermuscular space to the anterolateral femoral skin. The area extends about 12 x 30 cm. Within the flap, the anterior branch of the anterolateral cutaneous nerve of the high is located. This flap has been widely used for free transplantation in China since 1983 with satisfactory results. 相似文献
186.
187.
我国沙漠中部地区主要不同生态类型植物脯氨酸的累积、光合、呼吸和叶绿素含量 总被引:21,自引:0,他引:21
中生植物脯氨酸含量为0.42mg/g.dw, 低于少浆旱生植物(1.73mg/g.dw)。多浆旱生植物脯氨酸含量最高(7.22mg/g.dw),为前两者的17倍和4倍,两类旱生植物在干旱条件下(非灌溉)脯氨酸含量均高于灌水处理。少浆与多浆旱生植物的光合强度(16.74,14.04CO2mg/g.dw.h)差异不大,而中生植物(37.57mg/g.dW.h)略高于多浆旱生植物(4.73CO2Mg/g.dw.h),与中生植物(7.60Co2mg/g.dw.h)接近,光合/呼吸值,少浆,多浆与中生植物分别为2.50,3.09和4.59,说明中生植物的合成明显大于消耗,季节动态中,中生植物显著高于两类旱生植物,叶绿素总量三类植物差异甚微。 相似文献
188.
除虫菊的染色体数目及其核型 总被引:6,自引:0,他引:6
杜冰群;刘启宏;朱翠英 《武汉植物学研究》1988,6(1):95-96
除虫菊(Pyrethrum cinerariifolium Trev.)为菊科小黄菊属多年生宿根草本植物。以花或全草入药。除虫菊的主要有效成分为除虫菊酯和瓜叶除虫菊酯,可用于加工成各种制剂作杀虫剂原料,用以防治日常虫豸(蚊、蝇、虱等)和农业害虫,且对人 相似文献
189.
190.