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Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3 总被引:1,自引:0,他引:1
Cornillot JD; Pontet M; Dupuy C; Chadli A; Caron M; Joubert-Caron R; Bourin P; Bladier D 《Glycobiology》1998,8(5):425-432
Antisera raised against galectin-1 exhibit crossreactivities with other
galectins or related molecules. In order to overcome this problem, a
monoclonal antibody to human brain galectin-1 was obtained by selecting
clones without reactivity toward galectin-3. This mAb specifically bound
galectin-1 of various animal origins but neither galectin-2 nor galectin-3.
Western-blotting analysis of soluble human brain extracts after 2D gel
electrophoresis revealed only the two most acidic isoforms of galectin-1.
The ability of this mAb to bind galectin-1/asialofetuin complexes indicates
that its epitope is not localized in the carbohydrate recognition domain of
galectin-1. This particularity induces with efficiency its monospecificity.
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K Kertesz-Chaloupková PJ Walser JD Granado M Aebi U Kües 《Fungal genetics and biology : FG & B》1998,23(1):95-109
Monokaryotic mycelia of the homobasidiomycete Coprinus cinereus form asexual spores (oidia) constitutively in abundant numbers. Mycelia with mutations in both mating type loci (Amut Bmut homokaryons) also produce copious oidia but only when exposed to blue light. We used such an Amut Bmut homokaryon to define environmental and inherent factors that influence the light-induced oidiation process. We show that the Amut function causes repression of oidiation in the dark and that light overrides this effect. Similarly, compatible genes from different haplotypes of the A mating type locus repress sporulation in the dark and not in the light. Compatible products of the B mating type locus reduce the outcome of light on A-mediated repression but the mutated B function present in the Amut Bmut homokaryons is not effective. In dikaryons, the coordinated regulation of asexual sporulation by compatible A and B mating type genes results in moderate oidia production in light. Copyright 1998 Academic Press. 相似文献
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An anchored restriction-mapping approach applied to the genetic analysis of the Anopheles gambiae malaria vector complex 1 总被引:1,自引:0,他引:1
We introduce here a simple approach for rapidly determining restriction
maps for a number of regions of a genome; this involves "anchoring" a map
with a rare restriction site (in this case the seldom-cutting EagI)
followed by partial digestion of a frequent-cutting enzyme (e.g., Sau 3A).
We applied this technology to five species of the Anopheles gambiae
complex. In a single Southern blot we obtained about a 15-kb restriction
map each for the mtDNA, rRNA gene, and a scnDNA region for each of five
species. Phylogenetic analyses of these regions yield trees at odds with
the more traditional chromosome inversion-based trees. The value of the
approach for systematic purposes is the ease with which several large,
independent regions of the genome can be quickly assayed for molecular
variation.
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Testing lack of fit in multiple regression 总被引:2,自引:0,他引:2
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Background
Quantifying the amount of standing genetic variation in fitness represents an empirical challenge. Unfortunately, the shortage of detailed studies of the genetic architecture of fitness has hampered progress in several domains of evolutionary biology. One such area is the study of sexual selection. In particular, the evolution of adaptive female choice by indirect genetic benefits relies on the presence of genetic variation for fitness. Female choice by genetic benefits fall broadly into good genes (additive) models and compatibility (non-additive) models where the strength of selection is dictated by the genetic architecture of fitness. To characterize the genetic architecture of fitness, we employed a quantitative genetic design (the diallel cross) in a population of the seed beetle Callosobruchus maculatus, which is known to exhibit post-copulatory female choice. From reciprocal crosses of inbred lines, we assayed egg production, egg-to-adult survival, and lifetime offspring production of the outbred F1 daughters (F1 productivity). 相似文献39.
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