The serotonergic system is involved in feeding inhibition by pymetrozine. Comparative studies on a locust (Locusta migratoria) and an aphid (Myzus persicae)

Pymetrozine inhibits feeding in aphids immediately after application without producing visible neurotoxic effects, as previously reported. In the present work, Locusta migratoria, though not a plant-sucking insect, was found to respond to pymetrozine by displaying unique symptoms, which were lifting and stretching of the hindlegs, in addition to inhibition of feeding. In locust, pymetrozine enhanced spontaneous spike discharge of the metathoracic and suboesophageal ganglia in situ at nanomolar concentrations. Similarly, pymetrozine increased the spontaneous rhythmic contractions of the isolated foregut with maximal effects also in the nanomolar range. The actions of pymetrozine were counteracted by biogenic amine receptor antagonists and mimicked by serotonin, not by dopamine and octopamine. Moreover, pymetrozine and serotonin strongly potentiated the effects of each other. Pymetrozine was inactive at all neurotransmitter receptors present on isolated locust neuronal somata, and at all other examined neuronal sites. In Myzus persicae, electrical penetration graph experiments revealed that serotonin, like pymetrozine, inhibited stylet penetration, and strongly enhanced the action of pymetrozine, comparable to the locust. Amine receptor antagonists were not specifically active in the aphid. We conclude from the present results that pymetrozine acts via a novel mechanism that is linked to the signalling pathway of serotonin.     

The crystal structure of the major cat allergen Fel d 1, a member of the secretoglobin family

The domestic cat (Felis domesticus) is one of the most important causes of allergic asthma worldwide. The dominating cat allergen, Fel d 1, is composed of two heterodimers. Recently, it has been shown that recombinant Fel d 1, consisting of chain 2 and chain 1 fused together without additional linker, has immunological properties indistinguishable from the natural heterodimeric protein. Herein, we report the crystal structure of recombinant monomeric Fel d 1 at 1.85-A resolution, determined by multi-wavelength anomalous diffraction using selenomethionine substituted protein. Fel d 1 is an all-helical protein and consists of eight helices. The two halves of the recombinant Fel d 1 molecule, corresponding to the wild-type Fel d 1 chains, are very similar in three-dimensional structure, despite the lack of significant sequence identity. The structure of the Fel d 1 presents a striking similarity to that of uteroglobin, a steroid-inducible cytokine-like molecule with anti-inflammatory and immunomodulatory properties. An internal, asymmetric cavity is formed in the Fel d 1 that could bind an endogenous ligand. The distribution of residues lining this cavity suggests that such a ligand must be amphipathic. The structure of Fel d 1 displays the localization of three previously defined Fel d 1 IgE epitopes on the surface of the protein. The three-dimensional structure provides a framework for rational design of hypoallergenic mutants aimed for treatment of cat allergy.     

Anthropogenic Carbon Stock Dynamics of Pulp and Paper Products in Germany

Carbon‐based materials (CBMs) for energetic and material purposes combine biogenic and anthropogenic carbon cycles. In the latter, numerous manufactured products with various in‐use lifespans accumulate as anthropogenic carbon stocks. Understanding the behavior of these stocks is an important requirement to estimate not only future waste amounts, source for secondary raw materials, but also the impacts and effects in carbon emissions and carbon management. Previous models have estimated material stock changes; however, a lack of research in carbon stocks is perceived. Moreover, studies follow in‐use lifespan estimation approaches, such as decay functions, which do not coincide with observed consumption and waste treatment patterns. In the first part of this article, we present a carbon stock‐flow model to analyze inter‐relationships between carbon flows and stocks from raw materials to waste treatment processes considering a consumer perspective, where the dynamics of anthropogenic carbon stocks are completely described. In the second part, we study the pulp and paper industry in Germany under a scenario approach to analyze the behavior, development, and impacts of paper stocks and flows between 2010 and 2040. The model provided coherent results, with industrial data estimating 33.9 million metric tons in 2010 in paper stocks, equivalent to 410 kilograms per person. Consumption per capita and in‐use lifespan of products were identified as the most significant variables in carbon stock building. Model simulations show a sustained growth in stocks for the next 30 years, with increase in waste and carbon emissions. But in combination with recycling and reuse mechanisms and consumption patterns, environmental impacts are reduced.     

Ants medicate to fight disease

Parasites are ubiquitous, and the ability to defend against these is of paramount importance. One way to fight diseases is self‐medication, which occurs when an organism consumes biologically active compounds to clear, inhibit, or alleviate disease symptoms. Here, we show for the first time that ants selectively consume harmful substances (reactive oxygen species, ROS) upon exposure to a fungal pathogen, yet avoid these in the absence of infection. This increased intake of ROS, while harmful to healthy ants, leads to higher survival of exposed ants. The fact that ingestion of this substance carries a fitness cost in the absence of pathogens rules out compensatory diet choice as the mechanism, and provides evidence that social insects medicate themselves against fungal infection, using a substance that carries a fitness cost to uninfected individuals.     

Unraveling the Relationship between Trait Negative Affectivity and Habitual Symptom Reporting

Objective

In two studies, we aimed at further elucidating the relationship between trait negative affectivity (NA) and habitual symptom reporting (HSR) by relating these variables to measures of executive function, trait questionnaires, and effects of emotion induction.

Methods

Healthy female participants (N = 75) were selected on their scores for trait NA and for the Checklist for Symptoms in Daily Life. Three groups were compared: (1) low NA-low HSR; (2) high NA-low HSR; and (3) high NA-high HSR (low NA-high HSR did not occur). In study 1, participants underwent a Parametric Go/No-go Task and a Stroop Color-Word test, and trait questionnaires measured alexithymia and absorption. Forty-five participants (N = 15 in each group) were further engaged in study 2 to induce state NA using an affective picture paradigm.

Results

Impaired inhibition on the Stroop and Go/No go Task characterized high trait NA, but not high HSR, whereas alexithymia and absorption were elevated in HSR, regardless of trait NA. Negative picture viewing induced elevated state NA in all groups, but only high HSR also reported more bodily symptoms. This effect was moderated, but not mediated by state NA.

Conclusion

High trait NA is a vulnerability factor but not a sufficient condition to develop HSR. Deficient inhibition is related to the broad trait of NA, whereas the moderating effect of state NA on symptom reporting is specific for high HSR. Understanding processes related to alexithymia and absorption may specifically help to explain elevated HSR.     

Worker reproduction in Formica ants

A potential tragedy of the commons arises in social-insect colonies where workers are fertile if egg-laying workers decrease their contribution to other tasks. We studied worker ovary development and egg laying in relation to kin structure, colony size, and the presence of a queen in nine species (11 populations) of Formica ants. Workers were highly fertile and laid eggs in the presence of a queen in five out of the seven species where egg samples were obtained. Worker fertility correlated neither with colony size nor with kin structure, which suggests that colony-level costs and efficiency of policing precede relatedness as the most important conflict determinant. We conclude that careful quantification of the costs of worker reproduction and policing is essential for inferences about the tragedy of the commons.     

T cells that are naturally tolerant to cartilage-derived type II collagen are involved in the development of collagen-induced arthritis

The immunodominant T-cell epitope that is involved in collagen-induced arthritis (CIA) is the glycosylated type II collagen (CII) peptide 256-270. In CII transgenic mice, which express the immunodominant CII 256-270 epitope in cartilage, the CII-specific T cells are characterized by a partially tolerant state with low proliferative activity in vitro, but with maintained effector functions, such as IFN-γ secretion and ability to provide B cell help. These mice were still susceptible to CIA. The response was mainly directed to the glycosylated form of the CII 256-270 peptide, rather than to the nonglycosylated peptide. Tolerance induction was rapid; transferred T cells encountered CII within a few days. CII immunization several weeks after thymectomy of the mice did not change their susceptibility to arthritis or the induction of partial T-cell tolerance, excluding a role for recent thymic emigrants. Thus, partially tolerant CII autoreactive T cells are maintained and are crucial for the development of CIA.     

Early intervention with liraglutide improves glucose tolerance without affecting islet microcirculation in young Goto-Kakizaki rats

Liraglutide, an analog of glucagon-like peptide-1 (GLP-1), is an effective anti-diabetic agent with few side effects. Since native GLP-1 exerts vascular effects, we investigated changes in pancreatic islet blood flow using a non-radioactive microsphere technique, as well as insulin concentration and glucose tolerance after 17 day treatment with liraglutide in 6-week-old Goto-Kakizaki (GK) rats. Compared to saline-treated control GK rats, liraglutide limited body weight gain, decreased glycemia, improved glucose tolerance and lowered serum insulin concentration. Neither pancreatic or islet blood flow, nor pancreatic insulin content, was affected by liraglutide treatment. We conclude that early intervention with liraglutide decreases glycemia and improves glucose tolerance, thus halting the natural progression towards diabetes, without affecting islet microcirculation or pancreatic insulin content in young female GK rats.     

Über den Gerüchsinn der Mehlmottenschlupfwespe Habrobracon juglandis Ashmead

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