全文获取类型
收费全文 | 594篇 |
免费 | 53篇 |
国内免费 | 2篇 |
出版年
2023年 | 2篇 |
2021年 | 14篇 |
2020年 | 1篇 |
2019年 | 6篇 |
2018年 | 9篇 |
2017年 | 5篇 |
2016年 | 15篇 |
2015年 | 38篇 |
2014年 | 29篇 |
2013年 | 34篇 |
2012年 | 49篇 |
2011年 | 41篇 |
2010年 | 26篇 |
2009年 | 16篇 |
2008年 | 26篇 |
2007年 | 27篇 |
2006年 | 26篇 |
2005年 | 23篇 |
2004年 | 36篇 |
2003年 | 24篇 |
2002年 | 27篇 |
2001年 | 12篇 |
2000年 | 16篇 |
1999年 | 17篇 |
1998年 | 6篇 |
1997年 | 5篇 |
1996年 | 5篇 |
1995年 | 4篇 |
1994年 | 8篇 |
1993年 | 9篇 |
1992年 | 13篇 |
1991年 | 4篇 |
1990年 | 14篇 |
1989年 | 7篇 |
1988年 | 4篇 |
1987年 | 7篇 |
1986年 | 5篇 |
1985年 | 7篇 |
1984年 | 6篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1980年 | 5篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1976年 | 2篇 |
1975年 | 1篇 |
1973年 | 1篇 |
1971年 | 1篇 |
排序方式: 共有649条查询结果,搜索用时 171 毫秒
91.
92.
C Y Chen P Juo J S Liou C Q Li Q Yu J Blenis D V Faller 《Cell growth & differentiation》2001,12(6):297-306
Oncogenic Ras induces cells to undergo apoptosis after inhibition of protein kinase C (PKC) activity. The integration of differential signaling pathways is required for full execution of apoptosis. In this study, we used Jurkat as well as Fas/FADD-defective cell lines expressing v-ras to determine the upstream elements required for activation of the caspase cascade in PKC/Ras-mediated apoptosis. During this Ras-induced apoptotic process, caspase-8 was activated, possibly through its binding to Fas-associated death domain (FADD), in Jurkat/ras and Jurkat/Fas(m)/ras cells but not in Jurkat/FADD(m)/ras cells. c-Jun NH(2)-terminal kinase (JNK) was activated in all three cell lines expressing ras in response to apoptotic stimulation. Suppression of JNK by dn-JNK1 blocked the interaction of FADD and caspase-8 and partially protected Jurkat/ras and Jurkat/Fas(m)/ras cells from apoptosis. However, dn-JNK1 had no effect on PKC/Ras-induced apoptosis in Jurkat/FADD(m)/ras cells. The results indicate that FADD/caspase-8 signaling is involved in PKC/Ras-mediated apoptosis, and JNK may be an upstream effector of caspase activation. 相似文献
93.
The goal of this study was to relate conformational changes in the N-terminal domain of chicken troponin I (TnI) to Ca2+ activation of the actin-myosin interaction. The two cysteine residues in this region (Cys48 and Cys64) were labeled with two sulfhydryl-reactive pyrene-containing fluorophores [N-(1-pyrene)maleimide, and N-(1-pyrene)iodoacetamide]. The labeled TnI showed a typical fluorescence spectrum: two sharp peaks of monomer fluorescence and a broad peak of excimer fluorescence arising from the formation of an excited dimer (excimer). Results obtained show that forming a binary complex of labeled TnI with skeletal TnC (sTnC) in the absence of Ca2+ decreases the excimer fluorescence, indicating a separation of the two residues. This reduction in excimer fluorescence does not occur when labeled TnI is complexed with cardiac TnC (cTnC). The latter causes only partial activation of the Ca2+-dependent myofibrillar ATPase. The binding of Ca2+ to the two N-terminal sites of sTnC causes a significant decrease in excimer fluorescence and an increase in monomer fluorescence in complexes of labeled TnI with skeletal TnC or TnC/TnT, while Ca2+ binding to site II of cTnC only causes an increase in monomer fluorescence but no change in excimer fluorescence. Thus a conformational change in the N-terminal region of TnI may be necessary for full activation of muscle contraction. 相似文献
94.
Thiopeptin, a new antibiotic, inhibits T factor-dependent binding of phe-tRNA to ribosomes, without appreciable inhibition of GTP-Tu-phe-tRNA complex formation. GTP hydrolysis coupled with the phe-tRNA binding is affected by the antibiotic. It also interferes with fusidic acid-sensitive hydrolysis of GTP caused by interaction with G factor and ribosomes. Siomycin acts similarly. 相似文献
95.
96.
Chih-Wei?Chen Yueh-Lun?Lee Jing-Ping?Liou Yu-Hsiu?Liu Chin-Wei?Liu Tsai-Yun?Chen Huei-Mei?HuangEmail author 《Apoptosis : an international journal on programmed cell death》2016,21(9):1008-1018
Imatinib, a Bcr-Abl-specific inhibitor, is effective for treating chronic myeloid leukemia (CML), but drug resistance has emerged for this disease. In this study, we synthesized a novel tubulin polymerization inhibitor, MPT0B206 (N-[1-(4-methoxy-benzenesulfonyl)-2,3-dihydro-1H-indol-7-yl]-formamide), and demonstrated its apoptotic effect and mechanism in imatinib-sensitive K562 and imatinib-resistant K562R CML cells. Western blotting and immunofluorescence microscopy showed that MPT0B206 induced microtubule depolymerization in K562 and K562R cells. MPT0B206 inhibited the growth of these cells in a concentration- and time-dependent manner. It did not affect the viability of normal human umbilical vein endothelial cells. MPT0B206 induced G2/M cell cycle arrest and the appearance of the mitotic marker MPM-2 in K562 and K562R cells, which is associated with the upregulation of cyclin B1 and the dephosphorylation of Cdc2. Treatment of K562 and K562R cells with MPT0B206 induced apoptosis and reduced the protein levels of procaspase-9 and procaspase-3 and increased caspase-3 activity and PARP cleavage. MPT0B206 also reduced the levels of the antiapoptotic proteins Mcl-1 and Bcl-2 and increased the level of the apoptotic protein Bax. Additional experiments showed that MPT0B206 markedly downregulated Bcr-Abl mRNA expression and total and phosphorylated Bcr-Abl protein levels and inhibited the phosphorylation of its downstream proteins STAT5, MAPK, and AKT, and the protein level of c-Myc in K562 and K562R cells. Furthermore, MPT0B206 triggered viability reduction and apoptosis in CML cells carrying T315I-mutated Bcr-Abl. Together, these results suggest that MPT0B206 is a promising alternative for treating imatinib-resistant CML. 相似文献
97.
本文发表了中国西北部毛莨科新资料, 即巴里坤毛莨Ranunculus balikunensis J.G.Liou, sp.nov.;髯毛蒙古白头翁Pulsatilla ambigua Turcz.ex pritz.var.barbata J.G.Liou, var.nov.。 相似文献
98.
Liou Shou-Lu 《植物分类学报:英文版》1990,28(2):145-152
Five new taxa of the family Umbelliferae are described from China
They are Pimpinella filipedicellata S. L. Liou, Acronema yadongense S. L. Liou,
Sinocarum bijiangense S. L. Liou, Hydrocotyle salwinica var. obtusiloba S. L.
Liou, Cryptotaenia japonica f. pinnatisecta S. L. Liou. 相似文献
99.
Chromosome numbers are reported for 26 species and varieties of Umbelliferae
which belong to 3 subfamilies and 19 genera in this paper. Of these, 13 counts are
new records and some problems about chromosome numbers of Umbelliferae are simplydiscussed. 相似文献
100.